Compositions and methods for producing tobacco plants and products having reduced or eliminated suckers
The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.
1. A method for producing a modified tobacco plant comprising:
(a) introducing a recombinant DNA construct comprising a heterologous promoter that is operably linked to a polynucleotide encoding a polypeptide having an amino acid sequence at least 95% identical to SEQ ID NO: 233 to at least one tobacco cell, wherein said heterologous promoter is an axillary meristem-specific promoter functional in a central zone, a peripheral zone, a rib zone, or any combination thereof;
(b) selecting one or more tobacco cells from step (b) that comprise said recombinant DNA construct; and
(c) generating one or more modified plants from the one or more plant cells selected in step (b).
2. The method of claim 1 , wherein said heterologous promoter comprises a nucleic acid sequence having at least 95% sequence identity to a polynucleotide selected from the group consisting of SEQ ID NOs: 113, 115-118, 148, 149, 151, 153, 155, 157, 159, and 204.
3. The method of claim 1 , wherein said one or more modified plants comprise no suckers or reduced suckers as compared to a control plant lacking said recombinant DNA construct when grown under comparable conditions.
4. The method of claim 3 , wherein said reduced suckers comprises reduced sucker mass, reduced sucker length, or both.
5. The method of claim 1 , wherein said polypeptide has an amino acid sequence 100% identical to SEQ ID NO: 233.
6. The method of claim 1 , wherein said polypeptide is encoded by a nucleic acid sequence at least 95% identical to SEQ ID NO: 232.
7. A method of growing a modified tobacco plant comprising planting a modified tobacco seed comprising a heterologous promoter that is operably linked to a polynucleotide encoding a polypeptide having an amino acid sequence at least 95% identical to SEQ ID NO: 233 wherein said heterologous promoter is an axillary meristem-specific promoter functional in a central zone a peripheral zone a rib zone, or any combination thereof.
8. The method of claim 7 , wherein said heterologous promoter comprises a nucleic acid sequence having at least 95% sequence identity to a polynucleotide selected from the group consisting of SEQ 1D NOs: 113, 115-118, 148, 149, 151, 153, 155, 157, 159, and 204.
9. The method of claim 7 , wherein said one or more modified plants comprise no suckers or reduced suckers as compared to a control plant lacking said recombinant DNA construct when grown under comparable conditions.
10. The method of claim 9 , wherein said reduced suckers comprises reduced sucker mass, reduced sucker length, or both.
11. The method of claim 7 , wherein said polypeptide has an amino acid sequence 100% identical to SEQ ID NO: 233.
12. The method of claim 7 , wherein said polypeptide is encoded by a nucleic acid sequence at least 95% identical to SEQ ID NO: 232.
13. A method for producing a modified tobacco plant comprising:
(a) crossing at least one tobacco plant of a first tobacco variety with at least one tobacco plant of a second tobacco variety, where the at least one tobacco plant of the first tobacco variety comprises a recombinant DNA construct comprising a heterologous promoter that is operably linked to a polynucleotide encoding a polypeptide having an amino acid sequence at least 90% identical or similar to SEQ ID NO: 233 to produce at least one progeny tobacco plant, wherein said heterologous promoter is an axillary meristem-specific promoter functional in a central zone, a peripheral zone, a rib zone, or any combination thereof; and
(b) selecting at least one progeny tobacco plant produced in step (a) that comprises said recombinant DNA construct.
14. The method of claim 13 , wherein said heterologous promoter comprises a nucleic acid sequence having at least 95% sequence identity to a polynucleotide selected from the group consisting of SEQ ID NOs: 113, 115-118, 148, 149, 151, 153, 155, 157, 159, and 204.
15. The method of claim 13 , wherein said progeny tobacco plant comprise no suckers or reduced suckers as compared to a control plant lacking said recombinant DNA construct when grown under comparable conditions.
16. The method of claim 15 , wherein said reduced suckers comprises reduced sucker mass, reduced sucker length, or both.
17. The method of claim 13 , wherein said polypeptide has an amino acid sequence 100% identical or similar to SEQ ID NO: 233.
18. The method of claim 13 , wherein said polypeptide is encoded by a nucleic acid sequence at least 95% identical to SEQ ID NO: 232.
19. The method of claim 2 , wherein said heterologous promoter comprises a nucleic acid sequence having 100% sequence identity to a polynucleotide selected from the group consisting of SEQ NOs: 113, 115-118, 148, 149, 151, 153, 155, 157, 159, and 204.
20. The method of claim 8 , wherein said heterologous promoter comprises a nucleic acid sequence having 100% sequence identity to a polynucleotide selected from the group consisting of SEQ LD NOs: 113, 115-118, 148, 149, 151, 153, 155, 157, 159, and 204.
21. The method of claim 14 , wherein said heterologous promoter comprises a nucleic acid sequence having 100% sequence identity to a polynucleotide selected from the group consisting of SEQ ID NOs: 113, 115-118, 148, 149, 151, 153, 155, 157, 159, and 204.