Recombinant expression vector and lipid membrane structure having said vector encapsulated therein
The problem to be solved is to provide a novel expression vector capable of effectively expressing a target protein in mitochondria and suppressing undesirable expression of the target protein in cell organelles other than mitochondria. The present invention provides a recombinant expression vector for expressing a target protein in mitochondria of animal cells, and a lipid membrane structure having the vector encapsulated therein, wherein the recombinant expression vector has a promoter sequence exhibiting a transcription activity in the nuclei of animal cells, and has, under the control of the promoter sequence, a coding region which codes a target protein and includes one or more TGAs as codons corresponding to tryptophan. The recombinant expression vector according to the present invention can more efficiently and selectively express a target protein in mitochondria, and can be used as a more safe and effective drug for treating mitochondrial diseases.
1. A recombinant expression vector for expressing a target protein in mitochondria in an animal cell, comprising a promoter exhibiting a transcriptional activity in a cell nucleus in the animal cell and the mitochondria, and a coding region encoding the target protein under control of the promoter,
wherein the target protein is a mitochondrial endogenous protein encoded by the coding region in the expression vector,
wherein the coding region contains one or more TGAs as a codon corresponding to tryptophan, and
wherein the promoter is selected from the group consisting of Cytomegalovirus promoter, Simian virus 40 promoter, Rous Sarcoma virus promoter, EF1α promoter, and β-actin promoter.
2. The recombinant expression vector of claim 1 , further comprising a coding region of a mitochondrial genomic DNA at the 5′ terminal side of the coding region encoding the target protein.
3. The recombinant expression vector of claim 2 , wherein the coding region of the mitochondrial genomic DNA is a coding region of NADH dehydrogenase, subunit 4.
4. The recombinant expression vector of claim 1 , further comprising a base sequence corresponding to a mitochondrial tRNA at the 3′ terminal side of the coding region encoding the target protein.
5. The recombinant expression vector of claim 1 , wherein the promoter is a Cytomegalovirus promoter or Rous Sarcoma virus promoter.
6. The recombinant expression vector of claim 1 , wherein all codons corresponding to tryptophan in the coding region encoding the target protein are TGA.
7. A lipid membrane structure encapsulating the recombinant expression vector of claim 1 and a lipid membrane.
8. The lipid membrane structure of claim 7 , wherein the lipid membrane comprises sphingomyelin as a constitutive lipid.
9. The lipid membrane structure of claim 7 , further comprising a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 13 on a surface of the lipid membrane.
10. The lipid membrane structure of claim 7 , wherein the recombinant expression vector further comprises a coding region of a mitochondrial genomic DNA at the 5′ terminal side of the coding region encoding the target protein.
11. The lipid membrane structure of claim 10 , wherein the coding region of the mitochondrial genomic DNA is a coding region of NADH dehydrogenase, subunit 4.
12. The lipid membrane structure of claim 7 , wherein the recombinant expression vector further comprises a base sequence corresponding to a mitochondrial tRNA at the 3′ terminal side of the coding region encoding the target protein.
13. The lipid membrane structure of claim 7 , wherein the promoter is Cytomegalovirus promoter or Rous Sarcoma virus promoter.
14. The lipid membrane structure of claim 7 , wherein all codons corresponding to tryptophan in the coding region encoding the target protein are TGA.