Severe acute respiratory syndrome (SARS)-associated coronavirus diagnostics
The invention relates to the diagnosis of a SARS-associated coronavirus, such as a SARS CoV-2 infection and SARS-CoV infection, using the SARS-CoV and SARS-CoV-2 nucleocapsid proteins and antibodies binding to these proteins. The invention encompasses reagents, methods and kits for the detection of a SARS-associated coronavirus.
1. A method for detecting a SARS-associated coronavirus infection, comprising:
providing a recombinant SARS-CoV-1 N protein;
providing a biological sample from an individual or a patient suspected to be infected with a SARS-CoV-2 coronavirus;
contacting the SARS-CoV-1 N protein with the biological sample; and
visualizing the antigen-antibody complexes formed.
2. The method of claim 1 , wherein the recombinant SARS-CoV-1 N protein is produced by bacteria pIV2.3N/DH5|alpha| transformed with pIV2.3N, which were deposited under the terms of the Budapest Treaty at the Collection Nationale de Culture de Microorganismes (CNCM) at the Institut Pasteur, 25, Rue du Docteur Roux, 75724 Paris, FR, on Oct. 23, 2003, under the number I-3117.
3. The method of claim 1 , wherein it comprises detection of IgG or IgM or IgA.
4. The method of claim 1 , wherein it comprises detection of IgG, IgM and IgA.
5. The method of claim 1 , comprising an ELISA, lateral flow immunoassays, bead-based immunoassays, or multiplex bead-based immunoassays.
6. The method of claim 1 , wherein the SARS-CoV-1 N protein comprises the sequence of SEQ ID NO: 2.
7. The method of claim 1 , wherein the SARS-CoV-1 N protein consists of the sequence of SEQ ID NO: 2.
8. The method of claim 1 , wherein the biological sample is from an individual or a patient who has been shown to be infected by SARS CoV-2 by a nucleic acid detection test.
9. The method of claim 8 , wherein the recombinant SARS-CoV-1 N protein is produced by bacteria pIV2.3N/DH5|alpha| transformed with pIV2.3N, which were deposited under the terms of the Budapest Treaty at the Collection Nationale de Culture de Microorganismes (CNCM) at the Institut Pasteur, 25, Rue du Docteur Roux, 75724 Paris, FR, on Oct. 23, 2003, under the number I-3117.
10. The method of claim 8 , wherein it comprises detection of IgG or IgM or IgA.
11. The method of claim 8 , wherein it comprises detection of IgG, IgM and IgA.
12. The method of claim 8 , comprising an ELISA, lateral flow immunoassays, bead-based immunoassays, or multiplex bead-based immunoassays.
13. The method of claim 8 , wherein the SARS-CoV-1 N protein comprises the sequence of SEO ID NO:2.
14. The method of claim 8 , wherein the SARS-CoV-1 N protein consists of the sequence of SEO ID NO: 2.