Multi functional toxins
Pesticidal proteins are provided that combine two or more modes of action into a single molecule. These pesticidal proteins therefor exhibit greater efficacy and/or durability of resistance, for the purposes of pest control, and can be utilized for pest control by provision in the diet of a pest organism, or by topical application to crop plants and/or pests. Methods and compositions for producing and using such proteins are also provided.
1. A method for producing a modified pesticidal protein for controlling an infestation by a target pest in a crop, said method comprising:
introducing a cadherin binding protein into a TIC105 protein to bind to a cadherin receptor of a target pest, wherein introducing the cadherin binding protein comprises inserting the amino acid sequence of SEQ ID NO:9 in place of three residues from G342 to A344 of the TIC105 protein comprising SEQ ID NO:8, and
producing the protein;
wherein the target pest is selected from the group consisting of fall armyworm, cabbage looper, and soybean looper.
2. The method of claim 1 , further comprising providing the modified pesticidal protein within the diet of the target pest.
3. The method of claim 1 , further comprising topically applying the modified pesticidal protein to the crop or the target pest.
4. The method of claim 1 , further comprising expressing the modified pesticidal protein in a crop plant to control infestation of the target pest.
5. The method of claim 4 , wherein the modified pesticidal protein is encoded by a polynucleotide molecule incorporated in the genome of said plant.
6. The method of claim 2 , further comprising providing to the target pest a pesticidal agent that is different from the modified pesticidal protein and is selected from the group consisting of a bacterial toxin, a plant toxin, an arachnid toxin, a venom toxin, and a dsRNA targeting for suppression of an essential gene in said target pest.
7. The method of claim 6 , wherein said pesticidal agent is selected from the group consisting of Cry1A, Cry1Aa, Cry1Ab, Cry1A.105, Cry1B, Cry1Bb1, Cry1C, Cry1Ca, Cry1C amino acid sequence variants, Cry1D, Cry1Da, Cry1D amino acid sequence variants, Cry1E, Cry1F, Cry1Fa, Cry1G, Cry1H, Cry1I, Cry1J, Cry1K, Cry1L, Cry2A, Cry2Aa, Cry2Ab, Cry2Ae, Cry3, Cry3Aa, Cry3A amino acid sequence variants, Cry3B, Cry3Bb, Cry3Bb amino acid sequence variants, Cry4B, Cry5, Cry6, Cry7, Cry8, Cry9, Cry15, Cry34, Cry35, Cry43A, Cry43B, Cry51Aa1, ET29, ET33, ET34, ET35, ET66, ET70, Cry1A.105, TIC400, TIC407, TIC417, TIC431, TIC800, TIC807, TIC834, TIC853, TIC901, TIC1201, TIC1415, TIC3131, VIP3A, VIP3B, 5307, Axmi184, Axmi205, eHIP toxin proteins, insecticidal DIG proteins, venom proteins, and the insecticidal core toxin of each of the foregoing.
8. The method of claim 6 , wherein:
a) the pesticidal agent is toxic to the same pest as the modified pesticidal protein; or
b) the pesticidal agent is toxic to a different pest than the modified pesticidal protein.
9. The method of claim 3 , wherein the crop comprises a monocotyledonous plant selected from the group consisting of rice, wheat, barley, grasses, bentgrass, sugarcane, oats, sorghum, chives, shallots, and corn.
10. The method of claim 3 , wherein the crop comprises a dicotyledonous plant selected from the group consisting of cotton, canola, soybean, beans, sugarbeet, alfalfa, vegetables, fruits, curcubits, melons, pigeonpea, peppers, and peanut.