Platforms and systems for automated cell culture
Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.
1. A modular bioprocessing system, comprising:
(a) one or more cell culture process modules, each of the one or more cell culture process modules configured to:
(i) receive one or more cell culture containers, each of the one or more cell culture containers configured for performing one or more cell culture processes therein, wherein each of the one or more cell culture containers comprises at least one semi-transparent surface permanently attached thereto, wherein the at least one semi-transparent surface is configured to enable light-based cell imaging when illuminated with light within a first wavelength range and enable light-based cell manipulation when illuminated with light within a second wavelength range; and
(ii) manage and monitor cell culture functions of the one or more cell culture processes being performed within the one or more cell culture containers, wherein the managing and monitoring comprise directing the light within the second wavelength range toward adherent cells in the one or more cell culture containers, such that the directed light within the second wavelength range is at least partially absorbed by the at least one semi-transparent surface, to selectively lyse the adherent cells in the one or more cell culture containers;
(b) a server rack, wherein the one or more cell culture process modules are removably located on the server rack; and
(c) one or more shared subsystems on the server rack and supporting the one or more cell culture process modules.
2. The modular bioprocessing system of claim 1 , wherein each of the cell culture process modules is configured to removably couple to a cell culture cassette hosting the one or more cell culture processes via one or more pluggable connectors.
3. The modular bioprocessing system of claim 1 , wherein the one or more cell culture containers comprise a closed cassette system, a micro plate, a flask, a cell culture vessel, a microfluidic chamber, or any combination thereof.
4. The modular bioprocessing system of claim 3 , further comprising a front-facing instrument panel configured to receive or eject the closed cassette system, the micro plate, the flask, the cell culture vessel, the microfluidic chamber, or a combination thereof.
5. The modular bioprocessing system of claim 1 , further comprising a transporter configured to transport the one or more cell culture containers between locations within the server rack.
6. The modular bioprocessing system of claim 5 , wherein the transporter further comprises a rail, a linear actuator, a motor, a bearing, a wheel, or a combination thereof.
7. The modular bioprocessing system of claim 5 , wherein the transporter is configured to provide horizontal or vertical transportation of the one or more cell culture containers.
8. The modular bioprocessing system of claim 1 , wherein the one or more shared subsystems further comprise at least one of a computing subsystem, a data storage subsystem, an environmental control subsystem, a laser source subsystem, and a gas distribution subsystem.
9. The modular bioprocessing system of claim 1 , wherein the one or more cell culture process modules further comprise at least one of a cell imaging subsystem, a cell editing subsystem, and a temperature control subsystem.
10. The modular bioprocessing system of claim 9 , wherein the cell imaging subsystem further comprises a brightfield imaging system, a phase imaging system, a quantitative phase imaging system, a transmissive darkfield imaging system, a reflective darkfield imaging system, a fluorescent imaging system, or any combination thereof.
11. The modular bioprocessing system of claim 9 , wherein the cell imaging subsystem is configured to capture images of the one or more cell culture processes.
12. The modular bioprocessing system of claim 11 , wherein the one or more shared subsystems further comprise a computing subsystem configured to monitor the one or more cell culture processes based at least in part on performing machine learning analysis of the captured images of the one or more cell culture processes.
13. The modular bioprocessing system of claim 1 , wherein the server rack comprises one or more server rack units with standardized computer server rack sizes.
14. The modular bioprocessing system of claim 1 , further comprising a backup energy storage configured to provide uninterrupted power to the one or more cell culture process modules and the one or more shared subsystems.
15. The modular bioprocessing system of claim 1 , further comprising a temperature control subsystem configured to manage a temperature of at least one of the one or more cell culture processes or a reagent used in the one or more cell culture processes.
16. The modular bioprocessing system of claim 1 , further comprising a pH control subsystem configured to manage a pH of at least one of the one or more cell culture processes.
17. The modular bioprocessing system of claim 1 , further comprising a gas content control subsystem configured to manage a dissolved oxygen or carbon dioxide content of at least one of the one or more cell culture processes or a reagent used in the one or more cell culture processes.
18. The modular bioprocessing system of claim 1 , further comprising a media control subsystem configured to provide a media to, or extract a media from, at least one of the one or more cell culture containers.
19. The modular bioprocessing system of claim 1 , wherein the one or more cell culture processes further comprise cell reprogramming, cell differentiation, cell gene editing, cell incubation, cell expansion, cell sorting, cell purification, cell-based bioproduction, or a combination thereof.
20. The modular bioprocessing system of claim 1 , wherein the server rack has a multi-rack configuration.
21. The modular bioprocessing system of claim 1 , wherein the one or more shared subsystems are configured to perform the cell culture functions of the one or more cell culture processes.
22. The modular bioprocessing system of claim 1 , wherein the one or more cell culture process modules are located at a stationary location on the server rack while the one or more cell culture processes are performed within the one or more cell culture containers.
23. The modular bioprocessing system of claim 1 , wherein the one or more cell culture processes comprise culturing induced pluripotent stem cells (iPSCs).
24. The modular bioprocessing system of claim 1 , further comprising a laser source configured to direct laser energy within the second wavelength range toward the at least one semi-transparent surface, to selectively lyse the adherent cells in the one or more cell culture containers.
25. The modular bioprocessing system of claim 24 , wherein the at least one semi-transparent surface further comprises a coating, wherein the coating is configured to absorb the laser energy within the second wavelength range and convert the laser energy into thermal or mechanical energy, to selectively lyse the adherent cells in the one or more cell culture containers.