Method of glycation of a polypeptide
The present invention relates to compositions, particularly liquid compositions, comprising enzymes, methods of making the compositions, and uses of the same for making, e.g., dairy products.
1. A method of modifying by glycation a polypeptide having beta-galactosidase activity, comprising:
contacting the polypeptide with 30-90 wt % of a reducing sugar under conditions sufficient to produce a polypeptide modified by glycation,
wherein the reducing sugar is fructose, galactose, glucose, glyceraldehyde, ribose, xylose, cellobiose, maltose, or lactose, wherein the conditions comprise contacting the polypeptide in a solution at pH 5-8, for a time of 3-100 hours, at a temperature of 50-80° C., and wherein the polypeptide has at least 3% of lysine and arginine residues of the polypeptide modified by glycation.
2. The method of claim 1 , wherein the polypeptide has improved transgalactosylating activity as compared to a polypeptide having beta-galactosidase activity which has not been modified by glycation.
3. The method of claim 1 , wherein the polypeptide has at least 5% of lysine and arginine residues of the polypeptide modified by glycation.
4. The method of claim 1 , wherein the polypeptide is contacted with 40-65 wt. % of a reducing sugar.
5. The method of claim 1 , wherein the polypeptide is from subfamily 5 of glycosyl hydrolase family 2 (GH2_5).
6. The method of claim 1 , wherein the polypeptide is from subfamily 6 of glycosyl hydrolase family 2 (GH2_6).
7. The method of claim 1 , wherein the polypeptide is a Bacillus polypeptide.
8. The method of claim 1 , wherein the polypeptide is a Bacillus circulans polypeptide.
9. The method of claim 1 , wherein the polypeptide is a Bifidobacterium polypeptide.
10. The method of claim 1 , wherein the polypeptide is a Bifidobacterium bifidum polypeptide.
11. The method of claim 1 , wherein the polypeptide is a Kluyvermyces polypeptide.
12. The method of claim 1 , wherein the polypeptide is a Kluyvermyces lactis polypeptide.