System and self-metering cartridges for point of care bioassays
The invention is directed to devices and methods for performing rapid low-cost bioassays in self-contained disposable cartridges that provide efficient mixing of sample and reactants under a layer of liquid wax. Some embodiments additionally use gravity assisted distribution of sample and assay reagents in conjunction with an appliance containing all necessary valves, pneumatic sources, heat sources and detection stations.
1. A device for performing a bioassay on a biological sample to determine the presence or quantity of one or more target polynucleotides when connected to an appliance that provides pressure and vacuum sources and a detection station, the device comprising:
a planar body comprising a sample chamber, a first conduit, at least one reagent chamber, at least one metering chamber, at least one mixing chamber and at least one detection chamber, wherein:
the sample chamber has a first inlet accepting a biological sample or a sample fluid containing a biological sample, a vent port allowing the passage of air but not liquid, and an outlet connected to the first conduit, wherein the vent port is capable of being sealingly connected to a valve in the appliance;
the metering chamber (a) connected to and in fluid communication with a reagent chamber, (b) connected to and in fluid communication with the outlet of the sample chamber through the first conduit, (c) connected to a metering vent port, and (d) connected to and in fluid communication with a mixing chamber conduit, wherein the metering vent port is capable of being sealingly connected to a valve in the appliance;
the reagent chamber capable of containing assay reagents, the reagent chamber being connected to the metering chamber by a passage and connected to a reagent vent port allowing the passage of air but not liquid, wherein the reagent vent port is capable of being sealingly connected to a valve and pump in the appliance so that the reagent port is capable of accepting air pressure for forcing the assay reagents into the metering chamber;
the mixing chamber for mixing the biological sample or sample fluid with the assay reagents, the mixing chamber having a top and a bottom and being in fluid communication with the metering chamber by a passage connecting the bottom of the mixing chamber to the metering chamber, the mixing chamber being connected at its top to a mixing vent port that allows the passage of air but not liquid, wherein the mixing vent port is capable of being sealingly connected to a valve in the appliance, wherein the mixing chamber or the reagent chamber or both chambers contain a predetermined quantity of wax having a melting temperature such that the wax forms a bubble-preventing layer on a reaction mixture whenever the mixing chamber is above the melting temperature; and
the detection chamber is in fluid communication with the mixing chamber by a passage connecting the detection chamber to the bottom of the mixing chamber, and the detection chamber is connected at its top to a detection vent port that allows the passage of air but not liquid, wherein the detection vent port is capable of being sealingly connected to a valve and vacuum source in the appliance so that the detection port is capable of accepting a vacuum for drawing the mixture of assay reagents and biological sample or sample fluid into the detection chamber from the mixing chamber.
2. The device of claim 1 wherein said assay reagents are immobilized in said reagent chamber by wax barriers comprising said wax, and wherein said assay reagents and said wax of the wax barriers are capable of being released upon heating said reagent chamber to a temperature above said melting temperature of said wax.
3. The device of claim 1 wherein said passage connecting said detection chamber to said bottom of said mixing chamber comprises an amplification chamber for said target polynucleotides.
4. The device of claim 3 wherein said amplification chamber is configured to produce a predetermined temperature in the range of from 55° C. to 70° C.
5. The device of claim 3 wherein said amplification chamber is configured to produce a predetermined temperature in the range of from 30° C. to 60° C.
6. A device for performing a bioassay for one or more target polynucleotides comprising:
a sample chamber comprising a first inlet for accepting a biological sample, a vent port allowing the passage of air but not liquid, and an outlet connected to a first conduit;
a metering chamber (a) connected to and in fluid communication with a reagent chamber, (b) connected to and in fluid communication with the outlet of the sample chamber through the first conduit, (c) connected to a metering vent port, and (d) connected to and in fluid communication with a mixing chamber conduit;
a reagent chamber capable of containing assay reagents, the reagent chamber being connected to the metering chamber by a passage and connected to a reagent vent port allowing the passage of air but not liquid;
a mixing chamber for mixing the biological sample with the assay reagents, the mixing chamber having a top and a bottom and being in fluid communication with the metering chamber by a passage connecting the bottom of the mixing chamber to the metering chamber, the mixing chamber being connected at its top to a mixing vent port that allows the passage of air but not liquid, wherein the mixing chamber or the reagent chamber or both chambers contain a predetermined quantity of wax having a melting temperature such that the wax forms a bubble-preventing layer on a reaction mixture whenever the mixing chamber is above the melting temperature; and
a detection chamber is in fluid communication with the mixing chamber by a passage connecting the detection chamber to the bottom of the mixing chamber, and the detection chamber is connected at its top to a detection vent port that allows the passage of air but not liquid.
7. The device of claim 6 wherein said passage connecting said detection chamber to said bottom of said mixing chamber comprises an amplification chamber for said target polynucleotides.
8. The device of claim 7 wherein said amplification chamber is configured to produce a predetermined temperature in the range of from 55° C. to 70° C.
9. The device of claim 7 wherein said amplification chamber is configured to produce a predetermined temperature in the range of from 30° C. to 60° C.
10. The device of claim 6 wherein said assay reagents are immobilized in said reagent chamber by wax barriers comprising said wax, and wherein said assay reagents and said wax of the wax barriers are capable of being released upon heating said reagent chamber to a temperature above said melting temperature of said wax.