Protein-nanoparticle conjugate purification methods
Methods and kits for purifying protein-nanoparticle conjugates are provided. In some embodiments, a multimodal medium having a size exclusion mode and a capture mode is used to purify the protein-nanoparticle conjugates.
1. A kit for purifying a protein-nanoparticle conjugate from free protein, the kit comprising:
a column;
a multimodal medium having a size exclusion mode in which pores in the medium exclude the antibody-nanoparticle conjugate and a capture mode in which ligands or charged groups within the medium capture the free protein and the multimodal medium comprises a polysaccharide that is agarose and has hydroxyapatite microcrystals entrapped therein;
a surfactant; and
a polyalkylene glycol.
2. The kit of claim 1 , wherein the surfactant is a poloxamer surfactant.
3. The kit of claim 2 , wherein the polyalkylene glycol is polyethylene glycol.
4. The kit of claim 2 , wherein the poloxamer surfactant is a F-68 poloxamer surfactant.
5. The kit of claim 1 , wherein the nanoparticle is a polymer dot.
6. The kit of claim 5 , wherein the polymer dot is 5-100 nm in diameter and is a colloidal semiconducting polymer.
7. The kit of claim 5 , wherein the polymer dot is fluorescent.
8. The kit of claim 1 , further comprising a HEPES buffer.
9. The kit of claim 8 , wherein the poloxamer surfactant is a F-68 poloxamer surfactant and the polyalkylene glycol is polyethylene glycol.