Alcohol soluble protein gas-liquid interface self-assembled porous membrane and preparation method thereof
An alcohol soluble protein gas-liquid interface self-assembled porous membrane and a preparation method thereof are provided. The alcohol soluble protein gas-liquid interface self-assembled porous membrane is prepared from an alcohol soluble protein membrane storage solution by an anti-solvent method. The porous interface membrane is rapidly prepared by an alcohol soluble protein interface self-assembled one-step method and can be rapidly formed within 4 seconds, which greatly improves the preparation efficiency of the alcohol soluble protein membrane. The structure (size, pore diameter, micro porosity) of the alcohol soluble protein interface self-assembled porous membrane is precisely regulated and controlled by regulating and controlling process parameters, and a new preparation solution of an alcohol soluble protein base membrane, which is more efficient and has a modifiable structure compared with an alcohol soluble protein membrane prepared by a traditional solvent evaporation method, is developed.
1. A method for preparing an alcohol soluble protein gas-liquid interface self-assembled porous membrane, comprising the following steps:
S 1 , adding an alcohol soluble protein into a good solvent to be dissolved to obtain a membrane storage solution with an alcohol soluble protein concentration of 10 to 30 wt %;
S 2 , regulating and controlling a temperature of an anti-solvent to be 25° C. to 40° C.; and
S 3 , dropping the membrane storage solution in the S 1 into the anti-solvent in the S 2 to form a self-assembled porous membrane;
wherein pH of the anti-solvent is 3.0 to 10.0, and an ionic strength thereof is 0 to 700 mM;
wherein the good solvent comprises any one of 50% to 90% (v/v) of an aqueous ethanol solution, 60% to 90% (v/v) of an aqueous methanol solution, 60% to 100% (v/v) of an aqueous acetic acid solution and 70% to 90% (v/v) of an aqueous acetone solution; wherein 100% (v/v) of the aqueous acetic acid solution means a pure acetic acid;
wherein the anti-solvent comprises any one of water, 0.1% to 50% (v/v) of an aqueous ethanol solution, 0.1% to 60% (v/v) of an aqueous methanol solution, 0.1% to 60% (v/v) of an aqueous acetic acid solution and 0.1% to 70% (v/v) of an aqueous acetone solution;
titration of the anti-solvent in the S 3 is single titration, and a volume ratio of the membrane storage solution to the anti-solvent during titration is 1:(500 to 10,000).
2. The method according to claim 1 , wherein the alcohol soluble protein comprises any one of gliadin, zein, kafirin and hordein.
3. An alcohol soluble protein gas-liquid interface self-assembled porous membrane prepared by the method of claim 1 .
4. An alcohol soluble protein gas-liquid interface self-assembled porous membrane prepared by the method of claim 2 .