Vectors, genetically modified bacteria, and methods of making and using
Provided herein are a vector, a genetically modified bacterium including the vector, methods of making the bacterium, methods of using the bacterium, and kits including the bacterium. The vector includes a coding region encoding at least one antimicrobial peptide, and the antimicrobial peptide includes at least one lysin.
1. A vector comprising a coding region and a heterologous promoter operably linked to the coding region that encodes an antimicrobial peptide, wherein the antimicrobial peptide comprises catalytic domain of Lys2ConCat (SEQ ID NO:3) or Lys2Con20Cat (SEQ ID NO:4).
2. The vector of claim 1 , wherein the heterologous promoter comprises a chloride-inducible promoter or a Pon promoter.
3. The vector of claim 1 , wherein the vector comprises an additional coding region that is operably linked to a second promoter, and wherein the additional coding region encodes a modulator protein that regulates the expression of the coding region encoding the antimicrobial peptide.
4. A kit comprising the vector of claim 1 .
5. A vector comprising a coding region and a heterologous promoter operably linked to the coding region that encodes an antimicrobial peptide,
wherein the heterologous promoter comprises a chloride-inducible promoter,
wherein the antimicrobial peptide comprises a catalytic domain of Lys2ConCat (SEQ ID NO:3) or Lys2Con20Cat (SEQ ID NO:4),
wherein the coding region encoding the antimicrobial peptide is operably linked to a coding region encoding a signal peptide, and
wherein the vector comprises an additional coding region that is operably linked to a second promoter, and further wherein the additional coding region encodes a modulator protein
that regulates the expression of the coding region encoding the antimicrobial peptide and is
operably linked to the heterologous promoter.