Minimally-invasive measurement of esophageal inflammation
In some embodiment, the present invention is directed to a device for obtaining samples from a subject. In other embodiments, methods and devices of the present invention allow the evaluation of conditions of the gastrointestinal tract of a subject. Measurements may be utilized, for example, to diagnose a disease of the esophagus, to monitor inflammation of the esophagus, or to access the treatment of a disease of the esophagus. In one embodiment, the invention comprises a method for measuring esophageal inflammation comprising deploying a device into the esophagus of a subject, removing the device after a predetermined period of time, analyzing the device for a diagnostic indicator of esophageal inflammation and evaluating the diagnostic indicator to diagnose esophageal inflammation.
1. A device comprising a string having an esophageal segment, a capsule enclosing at least a portion of the string and having an opening, the string spanning the opening of the capsule, wherein the esophageal segment further comprises one or more capture agents.
2. The device according to claim 1 , wherein the esophageal segment of the string comprises absorbent fiber, mesh fiber or textured fiber.
3. The device according to claim 1 , wherein the string comprises an abrasive material.
4. The device according to claim 1 , wherein the capsule is dissolvable.
5. The device according to claim 1 , wherein the opening of the capsule is perforated.
6. The device according to claim 1 , wherein the capsule comprises a base and a cap.
7. The device according to claim 1 , wherein the string of the device is capable of being secured to a cheek of a subject when in use.
8. The device according to claim 1 , wherein the one or more capture agents bind to one or more analytes.
9. The device according to claim 1 , wherein the one or more capture agents comprise an antibody.
10. The device according to claim 1 , further comprising one or more diagnostic or health indicators associated with or bound to the string after deployment in a subject.
11. The device according to claim 1 , wherein the esophageal segment further comprises one or more diagnostic or health indicators associated with, or bound to, the one or more capture agents when the string is deployed in a subject, wherein the one or more diagnostic or health indicators comprise one or more of a major basic protein (MBP), an eosinophil cationic protein (ECP), an eosinophil peroxidase (EPO), an eosinophil-derived neurotoxin (EDN), CLC/Gal-10, other eosinophil granule protein, IL-6, IL-8, IL-1β, IL-5, INF-γ and other cytokines, a chemokine, a cellular infiltrate, pH, IgE, tryptase, other marker of an allergic response, a receptor molecule, an allergen, arachadonic acid products, other inflammatory marker, substance P, bradykinin, other neurotransmitter, eosinophils, leukotrienes, other mast cells, squamous epithelial cells, inflammatory cells, bacteria, yeast, other microorganisms, and nucleic acids or polypeptides from a microorganism.
12. A kit comprising the device according to claim 1 and at least one container.
13. A method for analyzing gastrointestinal microbiota of a subject, the method comprising: deploying the device according to claim 1 into a gastrointestinal tract of the subject; retrieving the device and analyzing the device for captured diagnostic or health indicators from the gastrointestinal tract.
14. The method according to claim 13 , assessing condition of the subject based on the captured diagnostic or health indicators.
15. The device according to claim 1 , wherein the string is a nylon fiber string.
16. The device according to claim 1 , wherein the device does not include drag material in the capsule.
17. The device according to claim 1 , wherein when deployed the esophageal segment of the string is located in an esophagus of a subject.
18. The device according to claim 1 , wherein the one or more capture agents are positioned on the esophageal segment of the string such that the one or more capture agents remain exposed to a gastrointestinal tract of a subject during withdrawal of the string from the subject.
19. The device according to claim 1 , wherein the one or more capture agents are positioned on the esophageal segment of the string such that the one or more capture agents are targeted to bind to one or more diagnostic or health indicators from an esophagus of a subject.
20. The device according to claim 19 , wherein when the one or more diagnostic or health indicators bind to the one or more capture agents, the one or more diagnostic or health indicators bound to the one or more capture agents remain exposed to a gastrointestinal tract of the subject during withdrawal of the string from the subject.