Compositions and uses thereof for treating disease or condition
The present application provides methods, imaging agents and kits for determination of the distribution and expression levels of an immune checkpoint ligand (such as PD-L1 or a PD-L1 like ligand) in an individual having a disease or condition. Anti-PD-L1 antibody agents, and methods for treating diseases or disorders by administering anti-PD-L1 antibody agents are also provided.
1. A method of treating a cancer in an individual, comprising administering to the individual an effective amount of an anti-PD-L1 antibody agent, wherein the anti-PD-L1 antibody agent comprises an antibody moiety comprising a heavy chain variable region (V H ) and a light chain variable region (V L ), wherein:
a) the V H comprises a heavy chain complementarity determining region (HC-CDR) 1 comprising the amino acid sequence of SEQ ID NO: 41, an HC-CDR2 comprising the amino acid sequence of SEQ ID NO: 42, and an HC-CDR3 comprising the amino acid sequence of SEQ ID NO: 43; and
b) the V L comprises a light chain complementarity determining region (LC-CDR) 1 comprising the amino acid sequence of SEQ ID NO: 44, an LC-CDR2 comprising the amino acid sequence of SEQ ID NO: 45, and an LC-CDR3 comprising the amino acid sequence of SEQ ID NO: 46.
2. The method of claim 1 , wherein the effective amount of the anti-PD-L1 antibody agent is 0.005 μg/kg to 5 g/kg of total body weight of the individual.
3. The method of claim 1 , wherein:
a) the V H comprises an amino acid sequence having at least 80% sequence identity to the amino acid sequence of any one of SEQ ID NOs: 1, 5, 9, 11, and 13; and/or
b) the V L comprises an amino acid sequence having at least 80% sequence identity to the amino acid sequence of any one of SEQ ID NOs: 3, 7, 15, 17 and 19.
4. The method of claim 3 , wherein the antibody moiety comprises:
(a) a V H comprising the amino acid sequence of SEQ ID NO: 9, and a V L comprising the amino acid sequence of SEQ ID NO: 15;
(b) a V H comprising the amino acid sequence of SEQ ID NO: 9, and a V L comprising the amino acid sequence of SEQ ID NO: 17;
(c) a V H comprising the amino acid sequence of SEQ ID NO: 9, and a V L comprising the amino acid sequence of SEQ ID NO: 19;
(d) a V H comprising the amino acid sequence of SEQ ID NO: 11, and a V L comprising the amino acid sequence of SEQ ID NO: 15;
(e) a V H comprising the amino acid sequence of SEQ ID NO: 11, and a V L comprising the amino acid sequence of SEQ ID NO: 17;
(f) a V H comprising the amino acid sequence of SEQ ID NO: 11, and a V L comprising the amino acid sequence of SEQ ID NO: 19;
(g) a V H comprising the amino acid sequence of SEQ ID NO: 13, and a V L comprising the amino acid sequence of SEQ ID NO: 15;
(h) a V H comprising the amino acid sequence of SEQ ID NO: 13, and a V L comprising the amino acid sequence of SEQ ID NO: 17; or
(i) a V H comprising the amino acid sequence of SEQ ID NO: 13, and a V L comprising the amino acid sequence of SEQ ID NO: 19.
5. The method of claim 1 , wherein the antibody moiety comprises an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 21 or 23.
6. The method of claim 1 , wherein the antibody moiety is chimeric or humanized.
7. The method of claim 1 , wherein the antibody moiety is selected from the group consisting of a single-chain Fv (scFv), a Fab, a Fab′, a F(ab′)2, an Fv fragment, a disulfide stabilized Fv fragment (dsFv), a (dsFv) 2 , a Fv-Fc fusion, an scFv-Fc fusion, an scFv-Fv fusion, a diabody, a tribody, and a tetrabody.
8. The method of claim 7 , wherein the antibody moiety is a scFv.
9. The method of claim 1 , wherein the antibody moiety comprises an Fc fragment.
10. The method of claim 9 , wherein the Fc fragment comprises H310A and H435Q mutations, wherein the amino acid positions are based on the Kabat numbering system.
11. The method of claim 9 , wherein the individual is a human.
12. The method of claim 1 , wherein the antibody moiety is a full-length antibody.
13. The method of claim 12 , wherein the antibody moiety has an isotype selected from the group consisting of an IgG, an IgM, an IgA, an IgD, and an IgE.
14. The method of claim 13 , wherein the Fc fragment is an Fc fragment of IgG.
15. The method of claim 14 , wherein the Fc fragment is an Fc fragment of IgG1 or IgG4.
16. The method of claim 1 , wherein the antibody agent is suitable for intravenous, intraperitoneal, intramuscular, subcutaneous, or oral administration.
17. The method of claim 1 , wherein the method further comprises administering an effective amount of a second agent.
18. The method of claim 17 , wherein the second agent is a chemotherapeutic agent.
19. A pharmaceutical composition comprising an anti-PD-L1 antibody agent and a pharmaceutical acceptable carrier, wherein the antibody agent comprises an antibody moiety comprising a heavy chain variable region (V H ) and a light chain variable region (V L ), wherein:
a) the V H comprises an HC-CDR1 comprising the amino acid sequence of SEQ ID NO: 41, an HC-CDR2 comprising the amino acid sequence of SEQ ID NO: 42, and an HC-CDR3 comprising the amino acid sequence of SEQ ID NO: 43; and
b) the V L comprises an LC-CDR1 comprising the amino acid sequence of SEQ ID NO: 44, an LC-CDR2 comprising the amino acid sequence of SEQ ID NO: 45, and an LC-CDR3 comprising the amino acid sequence of SEQ ID NO: 46.
20. A kit for treating a disease or condition in an individual, comprising the pharmaceutical composition of claim 19 and an instruction.