Recombinant varicella-zoster virus (VZV) vaccine
The present disclosure discloses a recombinant varicella-zoster virus (VZV) vaccine, including a fusion protein formed by an amino acid sequence of an extracellular domain of a recombinant glycoprotein gE of a live attenuated VZV strain (OKA strain) gene and an Fc fragment of human immunoglobulin. The present disclosure further provides preparation and use of the fusion protein, a corresponding recombinant gene, a eukaryotic expression vector, etc. The fusion protein of the present disclosure has prominent immunogenicity and can induce the high-level expression of neutralizing antibodies in serum.
1. A recombinant varicella-zoster virus (VZV) vaccine preparation, comprising a fusion protein formed by an amino acid sequence of an extracellular domain of a recombinant glycoprotein gE of a live attenuated VZV strain (OKA strain) gene and an Fc fragment of human immunoglobulin, wherein the fusion protein has an amino acid sequence shown in SEQ ID No. 1.
2. The vaccine preparation according to claim 1 , further comprising a vaccine adjuvant.
3. The vaccine preparation according to claim 2 , wherein the vaccine adjuvant is an aluminum hydroxide adjuvant, an aluminum phosphate adjuvant, or a mixture of aluminum hydroxide and aluminum phosphate adjuvants.
4. The vaccine preparation according to claim 1 , wherein each dosage unit of the vaccine preparation comprises 5 μg to 200 μg of the fusion protein.
5. The vaccine preparation according to claim 4 , wherein each dosage unit of the vaccine preparation comprises 10 μg to 100 μg of the fusion protein.
6. The vaccine preparation according to claim 5 , wherein each dosage unit of the vaccine preparation comprises 20 μg to 60 μg of the fusion protein.
7. The vaccine preparation according to claim 1 , further comprising other substances that can enhance immunogenicity, wherein the other substances that can enhance immunogenicity comprise, but are not limited to: phosphatidylcholine (PC), lecithin, 3D-MPL, long-chain fatty acid (ester), mineral oil, vegetable oil, sodium methylcellulose (MC-Na), sodium carboxymethylcellulose (CMC-Na), and cholesterol-containing liposome.
8. The vaccine preparation according to claim 1 , wherein the vaccine preparation is a lyophilized preparation.
9. The vaccine preparation according to claim 8 , wherein the lyophilized preparation is dissolved by an aluminum hydroxide adjuvant suspension before use, and then a resulting mixture is thoroughly mixed and injected intramuscularly or subcutaneously.
10. A recombinant gene capable of expressing the fusion protein according to claim 1 , wherein the recombinant gene has a DNA sequence shown in SEQ ID No. 2.