Cell culture device system and methods of use thereof
A container for use in growing, culturing and/or modifying cells has a primary container ( 10 ) and defines an internal lumen in which a wall element ( 8 ) of the primary container ( 10 ) is compressible, and wherein the container has an auxiliary container ( 16 ) in fluid communication with the primary container ( 10 ). The fact that the device has an auxiliary container 16 allows a number of separate reactions to be carried out within a single container.
1. A device for use in growing, culturing and/or modifying cells, the device comprising a primary container having a first base section, a first top section arranged substantially in parallel with the first base section and a first wall element arranged between the first top section and the first base section and defining a corresponding internal lumen of the first primary container, in which the first wall element of the primary container is compressible with respect to the first top and first base sections, and in which the primary container has at least one optionally sealable inlet, in which the first wall element of the primary container is composed of a flexible material and includes one or more folds; and wherein the device further comprises one or more auxiliary containers, each auxiliary container in the one or more auxiliary containers couples with a respective inlet or outlet on the first top section of the primary container, wherein each auxiliary container in the one or more auxiliary containers is external to the primary container and in direct fluid communication with the primary container, and wherein the one or more auxiliary containers is located on the first top section of the primary container, and wherein the primary container or each auxiliary container in the one or more auxiliary containers comprises a valve.
2. The device of claim 1 , wherein each auxiliary container in the one or more auxiliary containers comprises a second base section configured to detachably connect to the respective inlet or outlet of the primary container.
3. The device of claim 1 , wherein each auxiliary container in the one or more auxiliary containers comprises a second base section, a second top section arranged substantially in parallel with the second base section and a second wall element arranged between the second top section and the second base section and defining a corresponding internal lumen of the auxiliary container, in which the second wall element of the auxiliary container is compressible with respect to the second top section and second base section, and in which the auxiliary container has at least one optionally sealable inlet, in which the second wall element of the auxiliary container is composed of a flexible material; and wherein the auxiliary container is configured to be detachably connected to the respective inlet or an outlet of the primary container.
4. The device of claim 1 , wherein each auxiliary container in the one or more auxiliary containers comprises insulation configured to maintain the contents of that auxiliary container at a particular temperature.
5. The device of claim 1 , wherein the primary container or an auxiliary container in the one or more auxiliary containers has the valve on each opposing side of the respective primary container or auxiliary container.
6. The device of claim 5 , wherein each valve on the opposing sides of the respective primary container or auxiliary container are configured to interact by forming a channel when the respective primary container or auxiliary container is fully compressed.
7. The device of claim 1 , wherein the device comprises a plurality of auxiliary containers.
8. The device of claim 1 , wherein the one or more folds is one or more z-folds.