Serological assay for the detection of zika virus-specific antibodies utilizing overlapping peptides comprising an NS2B epitope
The current invention provides compositions, methods, and kits for detecting the exposure to and infection by certain viruses. Specifically, the current invention allows for the rapid differential serological detection of exposure to, and infection by viruses. In particular, the current invention allows for the rapid serological detection of exposure to, and infection by Zika virus (ZIKV).
1. A method for the serological detection of an antibody to Zika virus in a sample, comprising:
a. contacting the sample with a collection of isolated non-redundant peptides specific for antibodies to Zika virus, wherein the peptides are 5 amino acids to 20 amino acids in length of the sequences set forth in SEQ ID NO: 1 or SEQ ID NOS:14-22, under conditions sufficient to allow the binding of the antibody(ies) to the peptide(s);
b. washing antibodies that have not bound to the isolated non-redundant peptides;
c. labeling bound antibodies with a detectable label; and
d. detecting formation of an antibody-peptide complex comprising said one or more peptides in the collection,
wherein formation of the complex is indicative of an antibody to an epitope of a Zika antigen being present in the sample.
2. The method of claim 1 , wherein the sample is from a subject.
3. The method of claim 2 , wherein the subject is a human female.
4. The method of claim 3 , wherein the subject is pregnant.
5. The method of claim 2 , wherein the subject is a test subject which has been administered a Zika virus vaccine or immunomodulatory agent.
6. The method of claim 2 , wherein the subject had a seropositive result on an enzyme-linked immunosorbent assay (ELISA).
7. The method of claim 1 , wherein the sample is chosen from the group consisting of nasopharyngeal aspirate, blood, cerebrospinal fluid, saliva, serum, plasma, urine, sputum, bronchial lavage, pericardial fluid, and peritoneal fluid.
8. The method of claim 1 , wherein the collection of peptides is immobilized to a solid support.
9. The method of claim 1 , wherein the detecting step comprises performing an ELISA assay.
10. The method of claim 1 , wherein the collection of peptides comprises peptides which are 12 amino acids in length.
11. The method of claim 1 , wherein the collection of peptides comprises peptides of SEQ ID NOs: 14-22.
12. A method for the serological detection of an antibody to Zika virus in a sample, comprising:
a. contacting the sample with a collection of isolated non-redundant peptides specific for antibodies to Zika virus, wherein the peptides are 5 amino acids to 20 amino acids in length of the sequences set forth in SEQ ID NOs: 2-13 and over 50% reactive in Zika virus convalescent serum samples, under conditions sufficient to allow the binding of the antibody(ies) to the peptide(s);
b. washing antibodies that have not bound to the isolated non-redundant peptides;
c. labeling bound antibodies with a detectable label; and
d. detecting formation of an antibody-peptide complex comprising said one or more peptides in the collection,
wherein formation of the complex is indicative of an antibody to an epitope of a Zika antigen being present in the sample.
13. The method of claim 12 , wherein the collection of peptides comprises peptides 12 amino acids in length.
14. The method of claim 12 , wherein the sample is from a subject.
15. The method of claim 12 , wherein the subject is a human female.
16. The method of claim 12 , wherein the subject is pregnant.
17. The method of claim 12 , wherein the subject is a test subject which has been administered a Zika virus vaccine or immunomodulatory agent.
18. The method of claim 12 , wherein the sample is chosen from the group consisting of nasopharyngeal aspirate, blood, cerebrospinal fluid, saliva, serum, plasma, urine, sputum, bronchial lavage, pericardial fluid, and peritoneal fluid.
19. The method of claim 12 , wherein the collection of peptides is immobilized to a solid support.
20. The method of claim 12 , wherein the detecting step comprises performing an ELISA assay.