Patent
The present invention provides both compositions comprising Methylobacterium and compositions comprising Methylobacterium that are depleted of substances that promote growth of resident microorganisms on a lettuce plant or seed. Also provided are methods for improving lettuce production, methods of making the compositions, and methods of treating a lettuce plant or seed with the compositions comprising Methylobacterium.
1. A composition for treating a plant or plant part to improve plant yield, plant insect resistance, plant fungal disease resistance, and/or to improve lettuce production, wherein said composition is essentially free of contaminating microorganisms, and comprises (i) a deposited Methylobacterium selected from the group consisting of NLS0017 (NRRL B-50931), NLS0020 (NRRL B-50930), NLS0021 (NRRL B-50939), NLS0037 (NRRL B-50941), NLS0038 (NRRL B-50942), NLS0042 (NRRL B-50932), NLS0046 (NRRL B-50929), NLS0062 (NRRL B-50937), NLS0064 (NRRL B-50938), NLS0065 (NRRL B-50935), NLS0066 (NRRL B-50940), NLS0068 (NRRL B-50934), NLS0069 (NRRL B-50936), and NLS0089 (NRRL B-509330; (ii) a derivative of deposited Methylobacterium NLS0017 (NRRL B-50931), NLS0020 (NRRL B-50930), NLS0021 (NRRL B-50939), NLS0037 (NRRL B-50941), NLS0038 (NRRL B-50942), NLS0042 (NRRL B-50932), NLS0046 (NRRL B-50929), NLS0062 (NRRL B-50937), NLS0064 (NRRL B-50938), NLS0065 (NRRL B-50935), NLS0066 (NRRL B-50940), NLS0068 (NRRL B-50934), NLS0069 (NRRL B-50936), or NLS0089 (NRRL B-50933) obtained by selection or by mutagenesis and selection of said deposited Methylobacterium ; or (iii) a genetically transformed strain obtained from a deposited Methylobacterium selected from the group consisting of NLS0017 (NRRL B-50931), NLS0020 (NRRL B-50930), NLS0021 (NRRL B-50939), NLS0037 (NRRL B-50941), NLS0038 (NRRL B-50942), NLS0042 (NRRL B-50932), NLS0046 (NRRL B-50929), NLS0062 (NRRL B-50937), NLS0064 (NRRL B-50938), NLS0065 (NRRL B-50935), NLS0066 (NRRL B-50940), NLS0068 (NRRL B-50934), NLS0069 (NRRL B-50936), and NLS0089 (NRRL B-509330); and wherein said composition further comprises an agriculturally acceptable adjuvant, excipient, or combination thereof.
2. The composition of claim 1 wherein said composition is an essentially dry product having about 5% or less water content.
3. The essentially dry product of claim 2 wherein said product is dried by lyophilization or spray drying.
4. The composition of claim 1 wherein said composition further comprises a component that enhances product efficacy or enhances ease of product application to a plant or plant part.
5. The composition of claim 1 wherein the agriculturally acceptable excipient comprises at least one component selected from the group consisting of calcium bentonite, kaolin, china clay, talc, perlite, mica, vermiculite, silicas, quartz powder, montmorillonite clay and mixtures thereof.
6. The composition of claim 1 wherein said agriculturally acceptable adjuvant is selected from the group consisting of a wetter/spreader, sticker, penetrant, extender, and humectant.
7. The composition of claim 1 wherein said agriculturally acceptable excipient is selected from the group consisting of woodflours, clays, activated carbon, diatomaceous earth, fine-grain inorganic solids, and calcium carbonate.
8. The composition of claim 1 wherein said composition is adapted for use in treating a plant seed.
9. The composition of claim 1 wherein said Methylobacterium is present at a titer of about 1×10 6 CFU/gm to about 1×10 14 CFU/gm.
10. A plant or plant part that is coated with the composition of claim 1 .
11. The plant or plant part of claim 10 wherein said plant part is a seed.
12. The plant or plant part of claim 10 wherein said plant is a corn, soybean, tomato, fruit or lettuce plant.
13. The composition of claim 1 wherein said agriculturally acceptable adjuvant comprises a malto-dextrin.