Methods and systems for controlling oxidative stress
The present application describes methods and systems for measuring and controlling oxidative stress in animals and humans. The degree of oxidative stress can be measured directly by inducing all of the blood cells to produce excessive reactive oxygen species (ROS) by exposure to an elevated concentration sulfide or other ROS inducing chemical and measuring the fluorescence intensity of a fluorescent dye or color intensity of dye that reacts with ROS. Oxidative stress can be reduced by reducing dietary sulfur, consumption of a methanogenic probiotic, or apheresis methods to replace ROS-positive blood cells with normal blood cells. Plasma oxidative stress can be compared in venous and arterial blood samples to evaluate small vessel disease. Oxidative stress can be increased by increasing dietary sulfur or the use of an intravenous method that exposes blood cells to an elevated blood concentration of sulfide or other ROS inducing chemical.
1. A method for determining a degree of a manifestation indicative of disease, disorder or indication in a subject, comprising:
adding a first agent to a first volume of a blood sample collected from the subject, the first agent being configured to induce a reactive oxygen species (ROS) response in blood cells of the first volume;
during a first incubation step, incubating: (1) the first volume of blood with the first agent to induce production of ROS in blood cells of the first volume, and (2) a second volume of the blood sample, wherein each of the first and second volumes contains plasma;
during a dilution step, diluting both a first and second volumes, wherein said diluting is configured to reduce a concentration of ROS in the plasma of each of the first and second volumes;
during an isolation step, (1) isolating the plasma of the first volume of blood to produce a first volume of plasma, and (2) isolating the plasma of the second volume of blood to produce a second volume of plasma; and
determining a degree of blood oxidative stress in the subject by comparing the level of ROS response in the first volume and the second volume.
2. The method of claim 1 , further comprising a second incubation step, wherein the second incubation step comprises incubating the first volume and the second volume of blood, the second incubation step is performed following the first incubation step.
3. The method of claim 1 , further comprising a dilution step, wherein the dilution step comprises diluting both the first volume and the second volume of blood, wherein the diluting is configured to reduce a concentration of ROS in the plasma of each of the first and second volumes of blood.
4. The method of claim 1 , further comprising providing guidance to the subject for treatment of the disease, disorder or indication.
5. The method of claim 4 , wherein the guidance comprises at least one of the following: apheresis, other blood replacement procedures, dietary changes and pharmaceutical correction.
6. The method of claim 4 , wherein the disease, disorder or indication comprises at least one of the following: cancer, inflammatory disease, arthritis, and antibiotic resistance.
7. The method of claim 1 , further comprising providing guidance to treat the subject if the degree of blood oxidative stress is above a threshold or if the degree of blood oxidative stress is indicative of the disease, disorder or indication.
8. The method of claim 7 , wherein providing guidance to treat the subject comprises guidance related to at least one of the following: an apheresis, a blood replacement procedures, a dietary change, and a pharmaceutical correction.
9. The method of claim 1 , wherein the isolation step comprises centrifugation or filtration.
10. The method of claim 1 , wherein the first agent comprises at least one of the following: sodium hydrogen sulfide (NaHS), 4-demethoxydaunorubicin (idarubicin), and tert-Butyl hydroperoxide (TBHP).