Oxygen sensors
Oxygen sensing luminescent dyes, polymers and sensors comprising these sensors and methods of using these sensors and systems are provided.
1. A method for detecting an analyte comprising placing a tissue-integrating sensor under the skin of a mammalian subject;
wherein the sensor comprises a polymer comprising one or more residues of a luminescent dye and one or more residues of 2-hydroxyethyl methacrylate (HEMA), wherein the luminescent dye is a compound having the formula:
2. The method of claim 1 , wherein the polymer is a hydrogel.
3. The method of claim 2 , wherein the hydrogel further comprises one or more residues of tetraethyleneglycol dimethacrylate.
4. The method of claim 2 , wherein the hydrogel further comprises one or more residues of triethyleneglycol dimethacrylate.
5. The method of claim 1 , wherein the residue of the luminescent dye is present at a concentration from about 0.01 mM to about 5 mM.
6. The method of claim 1 , wherein the residue of the luminescent dye is present at the concentration of about 1 mM.
7. The method of claim 1 , wherein the analyte is oxygen.
8. The method of claim 1 , wherein the sensor is tissue-integrating.
9. The method of claim 8 , wherein the sensor generates detectable luminescent signal when placed under the skin of a mammalian subject.
10. The method of claim 9 , wherein the sensor generates detectable luminescent signal when placed up to about 5 mm deep under the skin of a mammalian subject.
11. The method of claim 9 , wherein the sensor generates detectable luminescent signal when placed more than 1 mm deep under the skin of a mammalian subject.
12. The method of claim 9 , wherein the mammalian subject is a human.
13. The method of claim 9 , wherein the sensor is stable in a mammalian tissue for longer than 1 week.
14. The method of claim 1 , further comprising an oxidase.
15. The method of claim 14 , wherein the oxidase is a glucose oxidase, ethanol oxidase, lactate oxidase, pyruvate oxidase, bilirubin oxidase, or histamine oxidase.
16. The method of claim 14 , wherein the analyte is oxygen consumed by the oxidase.
17. The method of claim 16 , wherein the oxidase is a glucose oxidase, ethanol oxidase, lactate oxidase, pyruvate oxidase, bilirubin oxidase, or histamine oxidase.
18. The method of claim 17 , wherein the sensor is tissue-integrating.