Prodrugs of cytotoxic active agents having enzymatically cleavable groups
The invention relates to novel prodrugs or conjugates of the general formula (Ia) in which La, n, R and D have the definitions given in the description and which have a structural motif reduced to an asparagine derivative as cleavage site for tumour-associated proteases such as legumain, in which cytotoxic drugs, for example kinesin spindle protein inhibitors, are released by legumain cleavage, and to the use of these prodrugs or conjugates for treatment and/or prevention of diseases, and to the use of these prodrugs or conjugates for production of medicaments for treatment and/or prevention of diseases, especially of hyperproliferative and/or angiogenic disorders, for example cancers. Reduction of the legumain-cleavable substrate peptide sequence to an asparagine derivative as structural motif, as a result of slowed legumain cleavage, achieves an increase in stability in the lysosomes of healthy organs while simultaneously maintaining the high anti-tumour effect.
1. A compound, or a salt, a solvate, or a salt of a solvate thereof, wherein the compound has one of the following structures:
wherein
AK 1 and AK 2 is an antibody or an antigen-binding fragment thereof, and
n is a number from 1 to 50.
2. The compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, wherein the antibody or the antigen-binding fragment thereof binds to an extracellular cancer target molecule.
3. The compound according to claim 2 , or a salt, a solvate, or a salt of a solvate thereof, wherein the antibody or the antigen-binding fragment thereof, after binding to its extracellular target molecule on t-h a target cell, is internalized by the target cell through the binding.
4. The compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, wherein the antibody is an anti-HER2 antibody, an anti-EGFR antibody, an anti-B7H3 antibody, an anti-TWEAKR antibody, or an antigen-binding fragment thereof.
5. The compound according to claim 4 , or a salt, a solvate, or a salt of a solvate thereof, wherein the anti-HER2 antibody is trastuzumab.
6. The compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, wherein the antibody
(i) is an anti-EGFR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 2, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 3 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 4, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 6, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 7 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 8,
(ii) is an anti-HER2 antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 12, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 13 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 14, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 16, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 17 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 18,
(iii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 22, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 23 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 24, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 26, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 27 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 28,
(iv) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 32, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 33 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 34, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 36, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 37 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 38,
(v) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 42, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 43 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 44, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 46, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 47 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 48,
(vi) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 52, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 53 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 54, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 56, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 57 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 58,
(vii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 62, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 63 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 64, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 66, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 67 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 68,
(viii) is an anti-HER2 antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 72, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 73 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 74, and a variable region of the light chain (VL) comprising the variable CDR I sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 76, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 77 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 78,
(ix) is an anti-HER2 antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 82, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 83 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 84, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 86, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 87 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 88,
(x) is an anti-B7H3 antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 92, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 93 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 94, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 96, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 97 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 98,
(xi) is an anti-B7H3 antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 102, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 103 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 104, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 106, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 107 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 108,
(xii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 112, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 113 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 114, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 116, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 117 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 118,
(xiii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 122, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 123 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 124, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 126, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 127 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 128,
(xiv) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 132, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 133 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 134, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 136, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 137 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 138,
(xv) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 142, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 143 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 144, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 146, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 147 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 148, or
(xvi) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) comprising the variable CDR1 sequence of the heavy chain (H-CDR1), as shown by SEQ ID NO: 152, the variable CDR2 sequence of the heavy chain (H-CDR2), as shown by SEQ ID NO: 153 and the variable CDR3 sequence of the heavy chain (H-CDR3), as shown by SEQ ID NO: 154, and a variable region of the light chain (VL) comprising the variable CDR1 sequence of the light chain (L-CDR1), as shown by SEQ ID NO: 156, the variable CDR2 sequence of the light chain (L-CDR2), as shown by SEQ ID NO: 157 and the variable CDR3 sequence of the light chain (L-CDR3), as shown by SEQ ID NO: 158,
or is an antigen-binding fragment of these antibodies.
7. The compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, wherein the antibody
(i) is an anti-EGFR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 1 and a variable region of the light chain (VL) as shown by SEQ ID NO: 5,
(ii) is an anti-HER2 antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 11 and a variable region of the light chain (VL) as shown by SEQ ID NO: 15,
(iii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 21 and a variable region of the light chain (VL) as shown by SEQ ID NO: 25,
(iv) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 31 and a variable region of the light chain (VL) as shown by SEQ ID NO: 35,
(v) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 41 and a variable region of the light chain (VL) as shown by SEQ ID NO: 45,
(vi) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 51 and a variable region of the light chain (VL) as shown by SEQ ID NO: 55,
(vii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 61 and a variable region of the light chain (VL) as shown by SEQ ID NO: 65,
(viii) is an anti-HER2 antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 71 and a variable region of the light chain (VL) as shown by SEQ ID NO: 75,
(ix) is an anti-HER2 antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 81 and a variable region of the light chain (VL) as shown by SEQ IDNO: 85,
(x) is an anti-B7H3 antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 91 and a variable region of the light chain (VL) as shown by SEQ IDNO: 95,
(xi) is an anti-B7H3 antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 101 and a variable region of the light chain (VL) as shown by SEQ ID NO: 105,
(xii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 111 and a variable region of the light chain (VL) as shown by SEQ ID NO: 115,
(xiii) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 121 and a variable region of the light chain (VL) as shown by SEQ ID NO: 125,
(xiv) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 131 and a variable region of the light chain (VL) as shown by SEQ ID NO: 135,
(xv) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 141 and a variable region of the light chain (VL) as shown by SEQ ID NO: 145, or
(xvi) is an anti-TWEAKR antibody comprising a variable region of the heavy chain (VH) as shown by SEQ ID NO: 151 and a variable region of the light chain (VL) as shown by SEQ ID NO: 155,
or is an antigen-binding fragment of these antibodies.
8. The compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, wherein the antibody
(i) is an anti-EGFR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 9 and a region of the light chain as shown by SEQ ID NO: 10,
(ii) is an anti-HER2 antibody comprising a region of the heavy chain as shown by SEQ ID NO: 19 and a region of the light chain as shown by SEQ ID NO: 20,
(iii) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 29 and a region of the light chain as shown by SEQ ID NO: 30,
(iv) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 39 and a region of the light chain as shown by SEQ ID NO: 40,
(v) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 49 and a region of the light chain as shown by SEQ ID NO: 50,
(vi) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 59 and a region of the light chain as shown by SEQ ID NO: 60,
(vii) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 69 and a region of the light chain as shown by SEQ ID NO: 70,
(viii) is an anti-HER2 antibody comprising a region of the heavy chain as shown by SEQ ID NO: 79 and a region of the light chain as shown by SEQ ID NO: 80,
(ix) is an anti-HER2 antibody comprising a region of the heavy chain as shown by SEQ ID NO: 89 and a region of the light chain as shown by SEQ ID NO: 90,
(x) is an anti-B7H3 antibody comprising a region of the heavy chain as shown by SEQ ID NO: 99 and a region of the light chain as shown by SEQ ID NO: 100,
(xi) is an anti-B7H3 antibody comprising a region of the heavy chain as shown by SEQ ID NO: 109 and a region of the light chain as shown by SEQ ID NO: 110,
(xii) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 119 and a region of the light chain as shown by SEQ ID NO: 120,
(xiii) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 129 and a region of the light chain as shown by SEQ ID NO: 130,
(xiv) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 139 and a region of the light chain as shown by SEQ ID NO: 140,
(xv) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 149 and a region of the light chain as shown by SEQ ID NO: 150, or
(xvi) is an anti-TWEAKR antibody comprising a region of the heavy chain as shown by SEQ ID NO: 159 and a region of the light chain as shown by SEQ ID NO: 160,
or is an antigen-binding fragment of these antibodies.
9. A pharmaceutical composition comprising the compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, in combination with an inert non-toxic pharmaceutically suitable excipient.
10. A method for treatment of hyperproliferative and/or angiogenic disorders, comprising administering to a human in need thereof an effective amount of the compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof.
11. A method for treatment of cancer and tumors, comprising administering to a human in need thereof an effective amount of the compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof.
12. A method for treatment of cancer and tumors, comprising administering to a human in need thereof an effective amount of the compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, in combination with one or more therapeutic approaches for cancer immunotherapy or with one or more drugs directed against a molecular target from cancer immunotherapy.
13. The compound according to claim 1 , or a salt, a solvate, or a salt of a solvate thereof, wherein the AK1 antibody or antigen-binding fragment thereof is bound via a cysteine residue of the antibody or antigen-binding fragment thereof, or the AK2 antibody or antigen-binding fragment thereof is bound via a lysine residue of the antibody or antigen-binding fragment thereof.
14. The compound according to claim 13 , or a salt, a solvate, or a salt of a solvate thereof, wherein the AK1 antibody or antigen-binding fragment thereof is bound via a cysteine residue of the antibody or antigen-binding fragment thereof.
15. The compound according to claim 13 , or a salt, a solvate, or a salt of a solvate thereof, wherein the AK2 antibody or antigen-binding fragment thereof is bound via a lysine residue of the antibody or antigen-binding fragment thereof.