Method for isolation and morphological analysis of cells
Provided is a method for cell isolation and morphological analysis. The method includes: providing a substrate with one or more wells; inserting cell isolation devices, each of which has one side patterned with one or more microwells, into the wells; introducing a liquid medium including cells into the wells; allowing the microwells of the cell isolation devices to come into contact with the inner surfaces of the wells such that some of the cells are isolated and trapped at the level of individual cells in the microwells; and observing the morphological changes of the isolated cells.
1. A cell isolation device for cell analysis comprising:
a body ( 120 ),
wherein,
the body ( 120 ) comprises a top portion ( 121 ) and a bottom portion ( 122 ), wherein the top portion ( 121 ) comprises a top surface ( 123 ) and an outer edge portion ( 125 ) and the bottom portion ( 122 ) has a bottom surface ( 124 ),
the body ( 120 ) is made of a swellable material,
bubble holes ( 126 ) are formed on a flank of the top portion ( 121 ),
one or more microwells are patterned at least on the bottom surface ( 124 ), and
the bottom portion and the top portion have a cylindrical shape, and the bottom portion ( 122 ) has a smaller diameter than the top portion ( 121 ).
2. The cell isolation device according to claim 1 , wherein the swellable material is capable of being swollen by an external stimulus, wherein the external stimulus is selected from the group consisting of electricity, light, sound, heat, magnetism, specific substances, and combinations thereof.
3. The cell isolation device according to claim 1 , wherein the swellable material is a hydrogel.
4. The cell isolation device according to claim 1 , wherein the top surface ( 123 ) is concave.
5. A kit for cell isolation and morphological analysis comprising a substrate having one or more wells and a plurality of the cell isolation devices and each of the plurality of the cell isolation devices is according to claim 1 and is inserted and arranged in each well of the substrate.