Methods and compositions for treating and protecting against porcine reproductive and respiratory syndrome virus
View Patent ↗The present disclosure provides compositions and methods for decreasing permissiveness to infection by PRRSV. The methods involve inserting peptides or oligopeptides into the CD163 SRCR domain 5 to disrupt the protein structure and thereby prevent PRRSV infection. The deletion of the exon 13 region of PSTII eliminates infection. The replacement of cysteine residues with alanines in SRCR5 as a means to disrupt disulfide bond formation. The final method is the deletion of, or amino acid substitution within the SRCR4-5 interdomain region.
1. A method of reducing susceptibility of a cell to infection by porcine reproductive and respiratory syndrome virus (PRRSV) comprising introducing a modification to the cell's genome, wherein the reducing susceptibility is in comparison to a cell that has not been modified, and wherein the modification comprise:
disrupting at least a portion of exon 13 sequence of CD163, wherein the modification does not comprise a disruption of exon 14 sequence of CD163, and wherein the modification is sufficient for the reducing susceptibility.
2. The method of claim 1 , wherein the modification results in deletion of at least 10 amino acids of a CD163 protein.
3. The method of claim 1 , wherein the modification comprises a complete deletion of the exon 13 sequence.
4. The method of claim 1 , wherein the cell with the modification is at least 50% less susceptible to the infection by PRRSV than the cell that has not been modified.