Methods and compositions related to triple-negative breast cancer
Embodiments are directed to kits and methods of treating a breast cancer patient with a glucocorticoid receptor antagonist with or without an anticancer agent or compound after the patient has been determined to be susceptible to treatment with the glucocorticoid receptor antagonist.
1. A method for treating a triple-negative, glucocorticoid receptor (GR) positive breast cancer patient comprising administering a glucocorticoid receptor (GR) inhibitor to the patient after the level of expression for at least 2 genes of ABHD5, ACSL3, APIAR, ASMTL, ATP2B1, B8S10, BCOR, Cl2orf29, CACNAIG, CCT6A, CDK7, CDKN2D, CHMP2B, COL4A6, COL7A1, CORO2B, CPNE6, CRYI, CUL4A, DDX18, DLAT, DLG4, EIF3J, ETF1, F2R, FGF5, GL12, GRM5, GRM6, HEATR3, HOMER1, HPS5, HSPA9, IMPACT, IP07, IQCC, KCTD3, KISS 1, LMNA, LYPLA1, MAPRE2, MAS1, MUC5AC, NAP1L1, NOL11, NOX5, PEX3, PGRMC2, PLCB4, POLO, PRPF39, RABGGTB, RMND1, RPL31, RRH, SCN3B, SEH1L, SERP1, SERPIND1, SLC4A4, SPATA5L1, SSB, SSBP3, SYT1, TBXA2R, TCEB1, TROAP, TSEN2, TYRO3, USEI, UTP14A, WDR43, WNTSA, and ZNF189 has been measured from a biological sample from the patient.
2. A method for measuring the level of gene expression in a triple-negative, glucocorticoid receptor (GR) positive breast cancer patient for GR inhibitor therapy comprising measuring the level of expression of at least 2 genes of ABHD5, ACSL3, APIAR, ASMTL, ATP2B1, B8S10, BCOR, Cl2orf29, CACNAIG, CCT6A, CDK7, CDKN2D, CHMP2B, COL4A6, COL7A1, CORO2B, CPNE6, CRYI, CUL4A, DDX18, DLAT, DLG4, EIF3J, ETF1, F2R, FGF5, GL12, GRM5, GRM6, HEATR3, HOMER1, HPS5, HSPA9, IMPACT, IP07, IQCC, KCTD3, KISS1, LMNA, LYPLAI, MAPRE2, MAS1, MUC5AC, NAP1L1, NOL11, NOX5, PEX3, PGRMC2, PLCB4, POLO, PRPF39, RABGGTB, RMND1, RPL31, RRH, SCN3B, SEH1L, SERP1, SERPIND1, SLC4A4, SPATA5L1, SSB, SSBP3, SYT1, TBXA2R, TCEB1, TROAP, TSEN2, TYRO3, USEI, UTP14A, WDR43, WNTSA, and ZNF 189 in a biological sample from the breast cancer patient and comparing the levels to a control level.
3. The method of claim 2 , wherein the level of expression of at least 5 genes on Table S2 has been measured in a sample from the patient.
4. The method of claim 3 , wherein the level of expression of at least 10 genes is measured in a sample from the patient.
5. The method of claim 4 , wherein the level of expression of at least 20 genes is been measured in a sample from the patient.
6. The method of claim 5 , wherein the level of expression of at least 40 genes is measured in a sample from the patient.
7. The method of claim 6 , wherein the level of expression of 74 genes is measured in a sample from the patient.
8. The method of claim 2 , wherein one or more control levels are altered expression levels from a control that is a GR antagonist-reversible transcriptional target.
9. The method of claim 2 , wherein one or more control levels are unaffected expression levels from a GR antagonist-reversible transcriptional target.
10. The method of claim 9 , wherein the level of expression of at least ETF1 and SSB are increased compared to the control.
11. The method of claim 9 , wherein the level of expression of at least two genes is decreased compared to the control.
12. The method of claim 11 , wherein the at least two genes are CACNAIG, CDKN2D, COL4A6, COL7A1, CORO2B, CPNE6, DLG4, FGF5, GI12, GRM5, GRM6, IQCC, KISS1, LMNA, MAPRE2, MAS1, MUCSAC, NOXS, POLQ, RRH, SCN3B, SERPIND1, SLC4A4, SSBP3, SYT1, TBXA2R, TROAP, and/or TYRO3.
13. The method of claim 8 , wherein the level of expression of at least two genes is equivalent to the control level.
14. The method of claim 2 , wherein the patient has not been administered dexamethasone.
15. The method of claim 2 , wherein the patient's sample is determined to have a level of GR transcription that is higher than a median or average level of GR expression levels in breast cancer cells.