IP Library › Granted Patent US 12,065,448
Granted Patent B2
US 12,065,448 · App. 17/831,716 · Granted Aug 20, 2024

Heteroaryl plasma kallikrein inhibitors

Inventors: Nikolaos Papaioannou (Newton, MA); Jeremy Mark Travins (Southborough, MA); Sarah Jocelyn Fink (Arlington, MA); John Mark Ellard (Buntingford, GB); Alastair Rae (Saffron Walden, GB)
Assignee: Takeda Pharmaceutical Company Limited
C07D519/00
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Quick Facts
Patent No.
US 12,065,448
App. No.
17/831,716
Granted
Aug 20, 2024
Kind
B2
Abstract

The present invention provides compounds and compositions thereof which are useful as inhibitors of plasma kallikrein and which exhibit desirable characteristics for the same.

Claims (48)

1. A method of treating diabetic macular edema or hereditary angioedema comprising administering to a patient in need thereof a compound of Formula (II):

or a pharmaceutically acceptable salt thereof,

wherein:

Het A is selected from a 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclylene having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur, a 5- to 6-membered monocyclic heteroarylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, a 7- to 10-membered saturated or partially unsaturated bicyclic heterocyclylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, and a 7- to 10-membered bicyclic heteroarylene having 1-5 heteroatoms selected from oxygen, nitrogen, or sulfur, wherein Het A is substituted with 0-4 R A groups;

each R A is independently selected from halogen, —CN, —C(R)═N(R), —C(O)R, —C(O) 2 R, —C(O)N(R) 2 , —NO 2 , —N(R)—N(R) 2 , —N(R) 2 , —N(R)C(O)R, —N(R)C(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)S(O) 2 R, —OR, —OC(O)R, —OC(O)N(R) 2 , —SR, —S(O)R, —S(O) 2 R, —S(O)N(R) 2 , —S(O) 2 N(R) 2 , or an optionally substituted group selected from C 1-6 aliphatic, phenyl, 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur;

L is an optionally substituted C 1-6 hydrocarbon chain, wherein 1-3 methylene units are independently replaced with -Cy-, —C(R) 2 -, —O—, —NR—, —C(O)—, —S(O) 2 -, —C(O)NR—, —NRC(O)—, —S(O) 2 NR-, and —NRS(O) 2 —;

-Cy- is 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclylene, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclylene having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur, or 5- to 6-membered heteroarylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur;

R′is selected from hydrogen, halogen, —OR, —NR 2 , —SR, and optionally substituted C 1-6 aliphatic; wherein R′ may be taken together with a monocyclic Het A to form an optionally substituted fused 7- to 10-membered saturated or partially unsaturated bicyclic heterocyclylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur;

R 1 , R 3 , R 4 , R 5 , R 6 , R 7 , and R 8 are independently selected from hydrogen, halogen, —CN, —C(R)═N(R), —C(O)R, —C(O) 2 R, —C(O)N(R) 2 , —NO 2 , —N(R)—N(R) 2 , —N(R) 2 , —N(R)C(O)R, —N(R)C(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)S(O) 2 R, —OR, —OC(O)R, —OC(O)N(R) 2 , —SR, —S(O)R, —S(O) 2 R, —S(O)N(R) 2 , —S(O) 2 N(R) 2 , or an optionally substituted group selected from C 1-6 aliphatic, phenyl, 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur;

each R is independently hydrogen or an optionally substituted group selected from C 1-6 aliphatic, phenyl, 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur;

or two R groups on the same carbon or nitrogen are taken together with their intervening atoms to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic ring having 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur, and 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur; and

n is 0 or 1.

2. The method according to claim 1 , wherein when L is —(CR 2 ) m NRC(O)- and m is 0 to 2, one of the following (a) or (b) applies:

(a) at least one of R 5 , R 6 , R 7 , and R 8 is CN; or

(b) R 1 is an optionally substituted saturated monocyclic heterocycle comprising 1-3 nitrogen atoms, with the proviso that the compound is not N-((7-chloroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-(2-oxopyrrolidin-1-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-pyrazole-4-carboxamide, N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-morpholinoimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-carboxamide, N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-(2-oxopyrrolidin-1-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-carboxamide, N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-(2-oxooxazolidin-3-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-carboxamide, or 4-(2-((4-(((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)carbamoyl)-1H-1,2,3-triazol-1-yl)methyl)-6-cyclopropylimidazo[1,2-a]pyridin-8-yl)morpholine-2-carboxylic acid.

3. The method according to claim 1 , wherein L is selected from the group consisting of —C(R) 2 NRC(O)-#, —C(R) 2 C(O)NRC(R) 2 -#, —C(R) 2 NRC(O)C(R) 2 -#, —C(R) 2 NRC(R) 2 -#, —C(R) 2 C(R) 2 NRC(R) 2 -#, —C(O)NRC(R) 2 -#, —C(R) 2 C(O)NR-#, —NRC(O)C(R) 2 -#, -CR 2 C(O)NRC(R) 2 -#, -SO 2 NRC(R) 2 -#, and —C(R) 2 NRSO 2 -#, wherein #represents the point of attachment to Het A .

4. The method according to claim 1 , wherein when L is —C(R) 2 NRC(O)-#or —C(R) 2 C(O)NR-#, one of the following (a) or (b) applies:

(a) at least one of R 5 , R 6 , R 7 , and R 8 is CN; or

(b) R 1 is an optionally substituted saturated monocyclic heterocycle comprising 1-3 nitrogen atoms, with the proviso that the compound is not N-((7-chloroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-(2-oxopyrrolidin-1-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-pyrazole-4-carboxamide, N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-morpholinoimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-carboxamide, N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-(2-oxopyrrolidin-1-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-carboxamide, N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-(2-oxooxazolidin-3-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-carboxamide, or 4-(2-((4-(((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)carbamoyl)-1H-1,2,3-triazol-1-yl)methyl)-6-cyclopropylimidazo[1,2-a]pyridin-8-yl)morpholine-2-carboxylic acid.

5. The method according to claim 1 , wherein Het A is a 5-membered monocyclic heteroarylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, wherein Het A is substituted with 0-2 R A groups.

6. The method according to claim 1 , wherein Het A is selected from:

wherein * represents to point of attachment to L.

7. The method according to claim 1 , wherein the compound has a structure of Formula (II-a) or Formula (II-b):

or a pharmaceutically acceptable salt thereof.

8. The method according to claim 1 , wherein a single instance of R A is C 1-6 aliphatic substituted with halogen.

9. The method according to claim 1 , wherein R 1 , R 3 , R 4 , R 5 , and R 6 are hydrogen.

10. The method according to claim 1 , wherein R 1 is an optionally substituted group selected from 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, sulfur, and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur.

11. The method according to claim 1 , wherein R 1 is an optionally substituted C 1-6 aliphatic.

12. The method according to claim 1 , comprising administering to a patient in need thereof a compound of Formula (III):

or a pharmaceutically acceptable salt thereof,

wherein:

Het A is selected from a 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclylene having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur, a 5- to 6-membered monocyclic heteroarylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, a 7- to 10-membered saturated or partially unsaturated bicyclic heterocyclylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, and a 7- to 10-membered bicyclic heteroarylene having 1-5 heteroatoms selected from oxygen, nitrogen, or sulfur, wherein Het A is substituted with 0-4 R A groups;

each R A is independently selected from halogen, —CN, —C(R)═N(R), —C(O)R, —C(O) 2 R, —C(O)N(R) 2 , —NO 2 , —N(R)—N(R) 2 , —N(R) 2 , —N(R)C(O)R, —N(R)C(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)S(O) 2 R, —OR, —OC(O)R, —OC(O)N(R) 2 , —SR, —S(O)R, —S(O) 2 R, —S(O)N(R) 2 , —S(O) 2 N(R) 2 , or an optionally substituted group selected from C 1-6 aliphatic, phenyl, 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur;

L is an optionally substituted C 1-6 hydrocarbon chain, wherein 1-3 methylene units are independently replaced with -Cy-, —C(R) 2 -, —O—, —NR—, —C(O)—, —S(O) 2 -, —C(O)NR—, —NRC(O)-, —S(O) 2 NR-, and —NRS(O) 2 —;

-Cy- is 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclylene, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclylene having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur, or 5- to 6-membered heteroarylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur;

R fus is fused with Het A to form a fused 7- to 10-membered saturated or partially unsaturated bicyclic heterocyclylene having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur;

R 1 , R 3 , R 4 , R 5 , R 6 , R 7 , and R 8 are independently selected from hydrogen, halogen, —CN, —C(R)═N(R), —C(O)R, —C(O) 2 R, —C(O)N(R) 2 , —NO 2 , —N(R)—N(R) 2 , —N(R) 2 , —N(R)C(O)R, —N(R)C(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)S(O) 2 R, —OR, —OC(O)R, —OC(O)N(R) 2 , —SR, —S(O)R, —S(O) 2 R, —S(O)N(R) 2 , —S(O) 2 N(R) 2 , or an optionally substituted group selected from C 1-6 aliphatic, phenyl, 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; and

each R is independently hydrogen or an optionally substituted group selected from C 1-6 aliphatic, phenyl, 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur;

or two R groups on the same carbon or nitrogen are taken together with their intervening atoms to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic ring having 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur, and 5- to 6-membered heteroaryl having 1-4 heteroatoms selected from oxygen, nitrogen, or sulfur.

13. The method according to claim 12 , wherein L is selected from the group consisting of —C(R) 2 NRC(O)-#, —C(R) 2 C(O)NRC(R) 2 -#, —C(R) 2 NRC(O)C(R) 2 -#, —C(R) 2 NRC(R) 2 -#, —C(R) 2 C(R) 2 NRC(R) 2 -#, —C(O)NRC(R) 2 -#, —C(R) 2 C(O)NR-#, —NRC(O)C(R) 2 -#, —CR 2 C(O)NRC(R) 2 -#, —SO 2 NRC(R) 2 -#, and —C(R) 2 NRSO 2 -#, wherein #represents the point of attachment to Het A .

14. The method according to claim 12 , wherein the compound has a structure of Formula (III-a) or Formula (III-b):

or a pharmaceutically acceptable salt thereof.

15. The method according to claim 12 , wherein substituents on an optionally substituted R 1 group are independently halogen, —(CH 2 ) 0-4 R ∘ , —(CH 2 ) 0-4 OR ∘ , or -(CH 2 ) 0-4 C(O)OR ∘ , wherein each R ∘ is independently hydrogen, C 1-6 aliphatic, or a 5-6 membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

16. The method according to claim 12 , wherein Het A is pyrazolediyl fused with R fus .

17. The method according to claim 12 , wherein Het A is triazolediyl fused with R fus .

18. The method according to claim 14 , wherein R 5 and R 6 are each independently halogen.

19. The method according to claim 1 , wherein the compound is: 2-(7-chloroimidazo[1,5-a]pyridin-1-yl)-N-((1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)acetamide (I-1), 1-(7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)-N-((1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-2,2,2-trifluoroethan-1-amine (I-2), 7-chloro-N-((1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-8-fluoroimidazo[1,5-a]pyridine-1-carboxamide (I-3), N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-pyrazole-4-sulfonamide (I-4), N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-sulfonamide (I-5), 7-chloro-N-((1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-8-fluoroimidazo[1,5-a]pyridine-1-sulfonamide (I-6), 7-chloro-N-(1-(1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)ethyl)-8-fluoroimidazo[1,5-a]pyridine-1-sulfonamide (I-7), N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-5-(6-cyclopropylimidazo[1,2-a]pyridin-2-yl)-6,7-dihydro-5H-pyrrolo[1,2-a]imidazole-2-carboxamide (I-8), N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-5-(6-cyclopropylimidazo[1,2-a]pyridin-2-yl)-6,7-dihydro-5H-pyrrolo[1,2-a]imidazole-3-carboxamide (I-9), N-((7-cyanoimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-pyrazole-4-carboxamide (I-10), 1-(7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)-N-((1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)methanamine (I-11), N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-(1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)ethan-1-amine (I-12), 2-(7-chloroimidazo[1,5-a]pyridin-1-yl)-N-(1-(1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)ethyl)acetamide (I-13), methyl 2-((4-((((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)methyl)-6-cyclopropylimidazo[1,2-a]pyridine-8-carboxylate (I-14), 2-((4-((((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)methyl)-6-cyclopropylimidazo[1,2-a]pyridine-8-carboxylic acid (I-15), N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-(1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-pyrazol-4-yl)-2,2,2-trifluoroethan-1-amine (I-16), ethyl 3-(2-((4-((((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)methyl)-6-cyclopropylimidazo[1,2-a]pyridin-8-yl)propanoate (I-17a), 3-(2-((4-((((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)methyl)-6-cyclopropylimidazo[1,2-a]pyridin-8-yl)propanoic acid (I-17b), 1-(7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)-N-((1-((6-cyclopropyl-8-(4H-1,2,4-triazol-4-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)methanamine (I-18), 2-(7-chloroimidazo[1,5-a]pyridin-1-yl)-N-(1-(1-((6-cyclopropylimidazo[1,2-a]pyridin-2-yl)methyl)-1H-pyrazol-4-yl)-2,2,2-trifluoroethyl)acetamide (I-19), or N-((7-chloro-8-fluoroimidazo[1,5-a]pyridin-1-yl)methyl)-1-((6-cyclopropyl-8-(4-methylpiperazin-1-yl)imidazo[1,2-a]pyridin-2-yl)methyl)-1H-1,2,3-triazole-4-carboxamide (I-20);

or a pharmaceutically acceptable salt thereof.

Assignments (7)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 6, 2022
From: FINK, SARAH JOCELYN
To: BIODURO LLC
Reel/Frame 060111/0256 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 6, 2022
From: PAPAIOANNOU, NIKOLAOS; TRAVINS, JEREMY MARK
To: SHIRE HUMAN GENETIC THERAPIES, INC.
Reel/Frame 060111/0267 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 6, 2022
From: ELLARD, JOHN MARK; RAE, ALASTAIR
To: CHARLES RIVER DISCOVERY RESEARCH SERVICES UK LIMITED
Reel/Frame 060111/0276 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 6, 2022
From: CHARLES RIVER DISCOVERY RESEARCH SERVICES UK LIMITED
To: CHARLES RIVER LABORATORIES, INC.
Reel/Frame 060111/0297 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 6, 2022
From: CHARLES RIVER LABORATORIES, INC.
To: SHIRE HUMAN GENETIC THERAPIES, INC.
Reel/Frame 060111/0310 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 6, 2022
From: BIODURO LLC
To: SHIRE HUMAN GENETIC THERAPIES, INC.
Reel/Frame 060111/0330 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 6, 2022
From: SHIRE HUMAN GENETIC THERAPIES, INC.
To: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Reel/Frame 060285/0748 →
Continuity (3)
Continuation 17024189 · Sep 17, 2020
Provisional Application 62902333 · Sep 18, 2019
Related Publication 20220298176A1 · Sep 22, 2022
Cited By (1)
US 12,441,724