High-transduction-efficiency rAAV vectors, compositions, and methods of use

1. An AAV VP3 protein comprising a non-native, non-threonine amino acid residue at a position corresponding to T491 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and:

(i) a non-native, non-serine amino acid residue at a position corresponding to S662 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;

(ii) a non-native, non-serine amino acid residue at a position corresponding to S662 and a non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;

(iii) a non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;

(iv) a non-native, non-tyrosine amino acid residue at a position corresponding to Y444 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;

(v) non-native, non-tyrosine amino acid residues at positions corresponding to Y444 and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;

(vi) non-native, non-tyrosine amino acid residues at positions corresponding to Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2; and/or

(vii) non-native, non-tyrosine amino acid residues at positions corresponding to Y272, Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;

wherein the non-native, non-threonine amino acid residue is not alanine.

2. The AAV VP3 protein of claim 1 , comprising the non-native, non-threonine amino acid residue at a position corresponding to T491 and the non-native, non-serine amino acid residue at a position corresponding to S662 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and

wherein non-native, non-threonine amino acid residue is valine; and the non-native, non-serine amino acid residue is valine.

3. The AAV VP3 protein of claim 1 , comprising the non-native, non-threonine amino acid residue at a position corresponding to T491, the non-native, non-serine amino acid residue at a position corresponding to S662, and the non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, an

wherein non-native, non-threonine amino acid residue is valine; the non-native, non-serine amino acid residue is valine; and the non-native, non-tyrosine amino acid residue is phenylalanine.

4. The AAV VP3 protein of claim 1 , comprising the non-native, non-threonine amino acid residue at a position corresponding to T491 and the non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and

wherein non-native, non-threonine amino acid residue is valine; and the non-native, non-tyrosine amino acid residue is phenylalanine.

5. The AAV VP3 protein of claim 1 , comprising the non-native, non-threonine amino acid residue at a position corresponding to T491 and the non-native, non-tyrosine amino acid residue at a position corresponding to Y444 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and

wherein non-native, non-threonine amino acid residue is valine; and the non-native, non-tyrosine amino acid residue is phenylalanine.

6. The AAV VP3 protein of claim 1 , comprising the non-native, non-threonine amino acid residue at a position corresponding to T491 and the non-native, non-tyrosine amino acid residues at positions corresponding to Y444 and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and

wherein non-native, non-threonine amino acid residue is valine; and the non-native, non-tyrosine amino acid residues are phenylalanine.

7. The AAV VP3 protein of claim 1 , comprising the non-native, non-threonine amino acid residue at a position corresponding to T491 and the non-native, non-tyrosine amino acid residues at positions corresponding to Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and

wherein non-native, non-threonine amino acid residue is valine, and the non-native, non-tyrosine amino acid residues are phenylalanine.

8. The AAV VP3 protein of claim 1 , comprising the non-native, non-threonine amino acid residue at a position corresponding to T491 and the non-native, non-tyrosine amino acid residues at positions corresponding to Y272, Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and

wherein non-native, non-threonine amino acid residue is valine, and the non-native, non-tyrosine amino acid residues are phenylalanine.

9. The AAV VP3 protein of claim 1 , wherein the AAV VP3 protein is an AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, or AAV12 serotype VP3 protein.

10. An isolated nucleic acid molecule that encodes the AAV VP3 protein of claim 1 .

11. A recombinant AAV (rAAV) particle comprising the AAV VP3 protein of claim 1 and a nucleic acid segment comprising a sequence that encodes a therapeutic agent and/or a diagnostic agent.

12. The rAAV particle of claim 11 , wherein the nucleic acid segment further comprises a promoter and/or an enhancer operably linked to the sequence that encodes the therapeutic agent and/or the diagnostic agent.

13. The rAAV particle of claim 12 , wherein the promoter is a heterologous promoter, a tissue-specific promoter, a constitutive promoter, an inducible promoter, or any combination thereof.

14. The rAAV particle of claim 12 , wherein the promoter is a cytomegalovirus (CMV) promoter, a desmin (DES) promoter, a beta-actin promoter, an insulin promoter, an enolase promoter, a BDNF promoter, an NGF promoter, an EGF promoter, a growth factor promoter, an axon-specific promoter, a dendrite-specific promoter, a brain-specific promoter, a hippocampal-specific promoter, a kidney-specific promoter, an elafin promoter, a cytokine promoter, an interferon promoter, a growth factor promoter, an alpha-1 antitrypsin promoter, a neural cell-specific promoter, a central nervous system cell-specific promoter, a peripheral nervous system cell-specific promoter, an interleukin promoter, a serpin promoter, a hybrid CMV promoter, a hybrid beta-actin promoter, an EF1 promoter, a U1a promoter, a U1b promoter, a Tet-inducible promoter, or a VP16-LexA promoter; and/or

wherein the enhancer is a CMV enhancer, a synthetic enhancer, a liver-specific enhancer, a vascular-specific enhancer, a brain-specific enhancer, a neural cell-specific enhancer, a lung-specific enhancer, a muscle-specific enhancer, a kidney-specific enhancer, a pancreas-specific enhancer, or an islet cell-specific enhancer.

15. The rAAV particle of claim 12 , wherein the therapeutic agent and/or the diagnostic agent comprises an adrenergic agonist, an anti-apoptosis factor, an apoptosis inhibitor, a cytokine receptor, a cytokine, a cytotoxin, an erythropoietic agent, a glutamic acid decarboxylase, a glycoprotein, a growth factor, a growth factor receptor, a hormone, a hormone receptor, an interferon, an interleukin, an interleukin receptor, a kinase, a kinase inhibitor, a nerve growth factor, a netrin, a neuroactive peptide, a neuroactive peptide receptor, a neurogenic factor, a neurogenic factor receptor, a neuropilin, a neurotrophic factor, a neurotrophin, a neurotrophin receptor, an N-methyl-D-aspartate antagonist, a plexin, a protease, a protease inhibitor, a protein decarboxylase, a protein kinase, a protein kinase inhibitor, a proteolytic protein, a proteolytic protein inhibitor, a semaphorin, a semaphorin receptor, a serotonin transport protein, a serotonin uptake inhibitor, a serotonin receptor, a serpin, a serpin receptor, a tumor suppressor, BDNF, CNTF, CSF, EGF, FGF, G-SCF, GM-CSF, gonadotropin, IFN, IFG-1, M-CSF, NGF, PDGF, PEDF, TGF, TGF-B2, TNF, VEGF, prolactin, somatotropin, XIAP1, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-10(187A), viral IL-10, IL-11, IL-12, IL-13, IL-14, IL-15, IL-16, IL-17, or IL-18.

16. The rAAV particle of claim 11 , wherein the rAAV particle is an AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, or AAV 12 serotype particle.

17. A method of transducing a cell, tissue, and/or organ of interest, the method comprising contacting the cell, tissue, and/or organ of interest with a composition comprising an effective amount of the rAAV particle of claim 11 .

18. The method of claim 17 , wherein the cell, tissue, and/or organ of interest comprises an endothelial, epithelial, vascular, liver, lung, heart, pancreas, intestinal, kidney, muscle, bone, dendritic, cardiac, neural, blood, brain, fibroblast and/or cancer cell.

19. A method of treating or ameliorating a symptom of a disease, injury, disorder, or dysfunction in a subject, the method comprising administering to the subject a composition comprising an effective amount of the rAAV particle of claim 11 , thereby treating or ameliorating the symptom of the disease, injury, disorder, or dysfunction in the subject.

20. The method of claim 19 , wherein the administering comprises intracerebro-ventricular administration, subarachnoid administration, intraspinal administration, epidural administration, intrasternal administration, intramuscular administration, intravenous administration, subcutaneous administration, intrathecal administration, intraperitoneal administration, pulmonary administration, intranasal administration, oral administration, administration by inhalation, transdermal administration, intradermal administration, transmucosal administration, intraorbital administration, intracardiac administration, transtracheal administration, subcuticular administration, intraarticular administration, subcapsular administration, or administration by direct injection into an organ or a tissue of the subject.

21. The method of claim 19 , wherein the disease, injury, disorder, or dysfunction comprises Alzheimer's disease; Huntington's disease; Tay-Sach's disease; Parkinson's disease; memory loss; trauma; motor impairment; neuropathy; arthritis; palsy; cystic fibrosis (CF); a neural disease; amyotrophic lateral sclerosis (ALS); multiple sclerosis (MS); muscular dystrophy (MD); a peptide or polypeptide deficiency; a peptide or polypeptide overexpression; a musculoskeletal or neuromuscular disease; a cancer, tumor, or other malignant growth; a neurological deficit dysfunction; an autoimmune disease; an articular disease; a cardiac or pulmonary disease; ischemia; stroke; cerebrovascular accidents; transient ischemic attacks (TIA); diabetes or other disease of the pancreas; cardiocirculatory disease or dysfunction; hypotension; hypertension; atherosclerosis; hypercholesterolemia; vascular damage or disease; or a biliary, renal or hepatic disease or dysfunction.