Reagent mixing system and methods
A method includes, under control of control circuitry implementing a mixing protocol, aspirating reagents from multiple different reagent reservoirs into a cache channel. Designated amounts of the reagents are automatically aspirated from the corresponding reagent reservoirs by corresponding sippers based on the mixing protocol implemented by the control circuitry. The method also includes discharging the reagents from the cache channel into a mixing reservoir, and mixing the reagents within the mixing reservoir to form a reagent mixture.
1. A system, comprising:
reagent reservoirs containing different reagents;
a mixing reservoir to receive one or more of the reagents;
multiple sippers including a nozzle sipper and multiple reagent sippers, the reagent sippers respectively extending into the reagent reservoirs such that respective distal tips of the reagent sippers contact the reagents in the reagent reservoirs, and the nozzle sipper extending into the mixing reservoir;
a cache channel positioned upstream of the mixing reservoir and extending between a pump end and a reservoir end, the pump end of the cache channel operatively connected to a pump;
a reagent selector valve fluidly connected to the reservoir end of the cache channel and fluidly connected to each of the sippers through corresponding fluid channels;
one of:
an inlet of the mixing reservoir for pre-loading of a sample template; or
a template reservoir containing a sample template and fluidly connected to the reagent selector valve through an other sipper and corresponding fluid channel;
a control circuitry operatively connected to the pump and the reagent selector valve, the control circuitry implementing a mixing protocol by controlling the pump and the reagent selector valve to automatically aspirate the reagents through the corresponding reagent sippers into the cache channel at designated amounts of the corresponding reagents based on the mixing protocol to pre-mix the reagents, the control circuitry subsequently i) controlling the pump and the reagent selector valve to discharge the reagents from the cache channel through the nozzle sipper into the mixing reservoir and mix the reagents with the sample template pre-loaded within the mixing reservoir to form a reagent mixture or ii) controlling the pump and the reagent selector valve to discharge the reagents from the cache channel through the nozzle sipper into the mixing reservoir and to discharge the sample template from the template reservoir directly to the mixing reservoir to form a reagent mixture.
2. The system of claim 1 , wherein the control circuitry controls the pump to mix the reagents within the mixing reservoir by aspirating a volume of the reagent mixture into the nozzle sipper and subsequently discharging the volume of the reagent mixture from the nozzle sipper back into the mixing reservoir.
3. The system of claim 2 , wherein the control circuitry controls the pump and the reagent selector valve to aspirate and subsequently discharge the reagent mixture within the mixing reservoir multiple times to mix the reagents.
4. The system of claim 2 , wherein the nozzle sipper contains a buffer fluid therein, the control circuitry controlling the pump to introduce air into the nozzle sipper prior to aspirating the reagent mixture into the nozzle sipper to define an air gap between the buffer fluid and the reagent mixture that is aspirated into the nozzle sipper to avoid mixing the buffer fluid and the reagent mixture.
5. The system of claim 1 , wherein an inner diameter of the nozzle sipper is smaller than respective inner diameters of the reagent sippers.
6. The system of claim 1 , wherein the control circuitry controls the pump and the reagent selector valve to aspirate the reagents into the cache channel one at a time in an ordered sequence and to repeat the ordered sequence at least once before the reagents in the cache channel are discharged into the mixing reservoir.
7. The system of claim 1 , wherein the control circuitry controls the pump and the reagent selector valve to aspirate a first volume of the reagents into the cache channel and to subsequently discharge a second, smaller volume of the reagents from the cache channel into the mixing reservoir such that a residual volume of the reagents defining an upstream buffer zone remains in the cache channel after discharging the reagents into the mixing reservoir.