Inline chemical agent addition for inline reaction with fluid sample for analytic determinations

1. A method comprising:

receiving a fluid sample from an autosampler with a valve system;

receiving a chemical agent with the valve system;

introducing, via a pump system, the fluid sample and the chemical agent inline via a mixing port of the valve system to produce a mixed sample;

transferring, via the pump system, the mixed sample to a sample holding loop fluidically coupled with the valve system;

holding the mixed sample within the sample holding loop for a holding period of time to permit a reaction between the fluid sample and the chemical agent;

directing, via the pump system, the mixed sample from the sample holding loop to an analytic instrument following expiration of the holding period of time;

subsequent to directing the mixed sample from the sample holding loop to the analytic instrument following expiration of the holding period of time, receiving a second fluid sample from the autosampler with the valve system;

introducing the second fluid sample and the chemical agent inline via the mixing port of the valve system to produce a second mixed sample; and

holding the second mixed sample within the sample holding loop for a second holding period of time to permit a reaction between the second fluid sample and the chemical agent, wherein the second holding period of time differs from the first holding period of time.

2. The method of claim 1 , wherein directing, via the pump system, the mixed sample from the sample holding loop to the analytic instrument following expiration of the holding period of time includes:

directing, via the pump system, the mixed sample from the sample holding loop through a separation column to separate one or more chemical species from respective other chemical species in the mixed sample and subsequently to the analytic instrument following expiration of the holding period of time.

3. The method of claim 1 , wherein the chemical agent is introduced to the valve system through action of at least one pump.

4. The method of claim 1 , wherein the chemical agent includes a chiral derivatization agent.

5. The method of claim 1 , further comprising:

directing the second mixed sample from the sample holding loop to the analytic instrument following expiration of the second holding period of time.

6. The method of claim 5 , further comprising:

subsequent to directing the second mixed sample from the sample holding loop to the analytic instrument following expiration of the second holding period of time, receiving a third fluid sample from the autosampler with the valve system;

introducing the third fluid sample and the chemical agent inline via the mixing port of the valve system to produce a third mixed sample; and

holding the third mixed sample within the sample holding loop for a third holding period of time to permit a reaction between the third fluid sample and the chemical agent, wherein the third holding period of time differs from each of the first holding period of time and the second holding period of time.

7. The method of claim 6 , further comprising:

directing the third mixed sample from the sample holding loop to the analytic instrument following expiration of the third holding period of time.

8. The method of claim 1 , wherein the analytic instrument includes a mass spectrometer.

9. The method of claim 8 , wherein the mass spectrometer includes an electrospray ionization mass spectrometer.

10. A method comprising:

receiving a fluid sample with a first valve;

receiving a chemical agent with the first valve;

introducing, via a pump system, the fluid sample and the chemical agent inline via a mixing port of the first valve to produce a mixed sample;

transferring, via the pump system, the mixed sample to a second valve;

directing the mixed sample to a sample holding loop fluidically coupled with the second valve;

holding the mixed sample within the sample holding loop for a holding period of time to permit a reaction between the fluid sample and the chemical agent;

directing, via the pump system, the mixed sample from the sample holding loop to an analytic instrument following expiration of the holding period of time;

subsequent to directing the mixed sample from the sample holding loop to the analytic instrument following expiration of the holding period of time, receiving a second fluid sample with the first valve;

introducing the second fluid sample and the chemical agent inline via the mixing port of the first valve to produce a second mixed sample;

transferring the second mixed sample to the second valve;

directing the second mixed sample to the sample holding loop; and

holding the second mixed sample within the sample holding loop for a second holding period of time to permit a reaction between the second fluid sample and the chemical agent, wherein the second holding period of time differs from the first holding period of time.

11. The method of claim 10 , wherein directing, via the pump system, the mixed sample from the sample holding loop to the analytic instrument following expiration of the holding period of time includes:

directing, via the pump system, the mixed sample from the sample holding loop through a separation column to separate one or more chemical species from respective other chemical species in the mixed sample and subsequently to the analytic instrument following expiration of the holding period of time.

12. The method of claim 10 , wherein the chemical agent is introduced to the valve system through action of at least one pump.

13. The method of claim 10 , wherein the chemical agent includes a chiral derivatization agent.

14. The method of claim 10 , further comprising:

directing the second mixed sample from the sample holding loop to the analytic instrument following expiration of the second holding period of time.

15. The method of claim 14 , further comprising:

subsequent to directing the second mixed sample from the sample holding loop to the analytic instrument following expiration of the second holding period of time, receiving a third fluid sample with the first valve;

introducing the third fluid sample and the chemical agent inline via the mixing port of the first valve to produce a third mixed sample;

transferring the third mixed sample to the second valve;

directing the third mixed sample to the sample holding loop; and

holding the third mixed sample within the sample holding loop for a third holding period of time to permit a reaction between the third fluid sample and the chemical agent, wherein the third holding period of time differs from each of the first holding period of time and the third holding period of time.

16. The method of claim 15 , further comprising:

directing the third mixed sample from the sample holding loop to the analytic instrument following expiration of the third holding period of time.

17. The method of claim 10 , wherein the analytic instrument includes a mass spectrometer.

18. The method of claim 17 , wherein the mass spectrometer includes an electrospray ionization mass spectrometer.