Elite event canola NS-B50027-4
The present embodiments relate to elite event NS-B50027-4, seeds and oils obtained from NS-B50027-4, progeny derived from NS-B50027-4, the genetic and phenotypic characteristics of NS-B50027-4, and compositions and methods for the identification of elite event NS-B50027-4. In particular, NS-B50027-4 is a transgenic canola line capable of producing at least 5% DHA in its seed oil.
1. A method of detecting the presence of NS-B50027-4 or progeny thereof comprising a transgenic locus of NS-B50027-4 in a sample comprising plant DNA, representative seed of NS B50027-4 having been deposited under ATCC Accession No. PTA-123186, wherein said method comprises:
(a) selecting a first primer comprising at least eleven contiguous nucleotides from a portion of SEQ ID NO:47 or a complement thereof and a second primer comprising at least eleven contiguous nucleotides from another portion of SEQ ID NO:47 or a complement thereof to provide forward and reverse primers that when used together in a nucleic acid amplification reaction produces an amplicon comprising SEQ ID NO:43;
(b) contacting the selected primers with a sample comprising plant DNA;
(c) performing a nucleic acid amplification reaction;
(d) detecting the presence or absence of an amplicon comprising SEQ ID NO:43;
wherein the presence of SEQ ID NO:43 identifies NS-B50027-4 or progeny thereof.
2. The method of claim 1 , wherein the first primer is SEQ ID NO:51 or a complement thereof and the second primer is NO:52 or a complement thereof.
3. The method of claim 1 , wherein the first primer is nucleotides 22-46 of SEQ ID NO:28 or a complement of nucleotides 22-46 of SEQ ID NO:28.
4. The method of claim 1 , wherein the first primer is SEQ ID NO:30 or a complement thereof.
5. A method of detecting the presence of NS-B50027-4 or progeny thereof comprising a transgenic locus of NS-B50027-4 in a sample comprising plant DNA, seed of NS B50027-4 having been deposited under ATCC Accession No. PTA-123186, comprising:
(a) selecting a first primer comprising at least eleven contiguous nucleotides from a portion of SEQ ID NO:48 or a complement thereof and a second primer comprising at least eleven contiguous nucleotides from another portion of SEQ ID NO:48 or a complement thereof to provide forward and reverse primers that when used together in a nucleic acid amplification reaction produces an amplicon comprising SEQ ID NO:44;
(b) contacting the selected primers with a sample comprising plant DNA;
(c) performing a nucleic acid amplification reaction;
(d) detecting the presence or absence of an amplicon comprising SEQ ID NO:44;
wherein the presence of SEQ ID NO:44 identifies NS-B50027-4 or progeny thereof.
6. A method of detecting the presence of NS-B50027-4 or progeny thereof comprising a transgenic locus of NS-B50027-4 in a sample comprising plant DNA, representative seed of NS B50027-4 having been deposited under ATCC Accession No. PTA-123186, comprising:
(a) selecting a first primer comprising at least eleven contiguous nucleotides from a portion of SEQ ID NO:49 or a complement thereof and a second primer comprising at least eleven contiguous nucleotides from another portion of SEQ ID NO:49 or a complement thereof to provide forward and reverse primers that when used together in a nucleic acid amplification reaction produces an amplicon comprising SEQ ID NO:45;
(b) contacting the selected primers with a sample comprising plant DNA;
(c) performing a nucleic acid amplification reaction;
(d) detecting the presence or absence of an amplicon comprising SEQ ID NO:45;
wherein the presence of SEQ ID NO:45 identifies NS-B50027-4 or progeny thereof.
7. A method of detecting the presence of NS-B50027-4 or progeny thereof comprising a transgenic locus of NS-B50027-4 in a sample comprising plant DNA, representative seed of NS B50027-4 having been deposited under ATCC Accession No. PTA-123186, comprising:
(a) selecting a first primer comprising at least eleven contiguous nucleotides from a portion of SEQ ID NO:50 or a complement thereof and a second primer comprising at least eleven contiguous nucleotide from another portion of SEQ ID NO:50 or a complement thereof to provide forward and reverse primers that when used together in a nucleic acid amplification reaction produces an amplicon comprising SEQ ID NO:46;
(b) contacting the selected primers with a sample comprising plant DNA;
(c) performing a nucleic acid amplification reaction;
(d) detecting the presence or absence of an amplicon comprising SEQ ID NO:46;
wherein the presence of SEQ ID NO:46 identifies NS-B50027-4 or progeny thereof.