Specimen processing systems and methods for holding slides
At least some embodiments of the technology are directed to an automated slide processing apparatus configured to apply at least one reagent to a specimen carried by a microscope slide. The slide processing station can include a support element with a support surface, at least one vacuum port, and a sealing member having a non-round shape. In an uncompressed state, the scaling member can extend upwardly beyond the support surface. In a compressed state, the scaling member can be configured to maintain an airtight seal with a backside of the microscope slide as the microscope slide is pulled against the support surface by a vacuum drawn via the at least one vacuum port.
1. An automated specimen processing system, comprising:
a slide staging device including—
a standby platform configured to receive a microscope slide and including a support surface configured to support a lower surface of the microscope slide; and
an alignment device having a first aligning member and a second aligning member configured to physically contact opposing sides of the microscope slide to slide the microscope slide along the support surface such that the microscope slide moves from a misaligned position to an aligned position; and
a transfer head configured to transport microscope slides from the standby platform to a specimen processing station, the transfer head having a head alignment feature configured to engage with at least one of a corresponding alignment feature of the slide staging device to maintain alignment of the transfer head with the microscope slide at the standby platform while the transfer head is moved toward and into physical contact with an upper surface of the microscope slide, which is supported by the support surface and located at the aligned position.
2. The automated specimen processing system of claim 1 , wherein:
the head alignment feature comprises first and second elongate rods extending away from the transfer head, and
the corresponding alignment feature of the slide staging device comprises first and second openings configured to receive the first and second elongate rods, respectively.
3. The automated specimen processing system of claim 2 , wherein the first and second elongate rods have tapered end portions configured to align the first and second elongate rods with the first and second openings, respectively.
4. The automated specimen processing system of claim 1 , wherein:
the head alignment feature comprises first and second openings in the transfer head, and
the corresponding alignment feature of the slide staging device comprises first and second elongate rods configured to be received within the first and second openings of the transfer head, respectively.
5. The automated specimen processing system of claim 4 , wherein the first and second elongate rods have tapered end portions configured to align the first and second elongate rods with the first and second openings, respectively.
6. The automated specimen processing system of claim 1 , wherein:
the head alignment feature comprises first and second protrusions extending away from the transfer head, and
the corresponding alignment feature of the slide staging device comprises first and second openings configured to receive the first and second protrusions, respectively.
7. The automated specimen processing system of claim 1 , wherein:
the head alignment feature comprises first and second openings in the transfer head, and
the corresponding alignment feature of the slide staging device comprises first and second protrusions configured to be received within the first and second openings of the transfer head, respectively.
8. The automated specimen processing system of claim 1 , wherein the transfer head further comprises a position sensor configured to sense the alignment of the transfer head with respect to the standby platform.
9. The automated specimen processing system of claim 1 , wherein the slide staging device further comprises a position sensor configured to sense the alignment of the transfer head with respect to the standby platform.
10. An automated specimen processing system, comprising:
a slide staging device configured to receive a microscope slide and including
a holding region configured to support the microscope slide, and
an alignment device having a first aligning member and a second aligning member, wherein the first and second aligning members are movable relative to one another to contact opposing sides of the microscope slide to move the microscope slide with a lower surface resting on the holding region from a misaligned position to an aligned position, the lower surface extends between the opposing sides; and
a transfer head configured to transport microscope slides from the slide staging device to a specimen processing station, the transfer head having a slide capture feature configured to
engage an upper surface of the microscope slide, which is supported by the holding region, and
retain the microscope slide during transport by the transfer head.
11. The automated specimen processing system of claim 10 , wherein the slide capture feature comprises a suction device including:
a sealing member positioned to contact the microscope slide when the transfer head is in an engaged position above the slide staging device;
a vacuum port within the sealing member; and
a vacuum source fluidly connected to the vacuum port and configured to provide suction at the vacuum port to provide an airtight seal between the sealing member and the microscope slide.
12. The automated specimen processing system of claim 11 , further comprising a sensor configured to detect the presence of a microscope slide retained by the transfer head.
13. The automated specimen processing system of claim 12 , wherein the sensor is one of an air pressure sensor or a light sensor.
14. The automated specimen processing system of claim 10 , wherein the slide capture feature comprises a mechanical gripper including first and second jaws configured to engage the microscope slide at opposing sides.
15. The automated specimen processing system of claim 10 , wherein the slide capture feature comprises a mechanical gripper including a clamp configured to engage the microscope slide.
16. The automated specimen processing system of claim 10 , wherein the slide capture feature comprises a mechanical gripper including a magnet configured to engage the microscope slide.
17. A method of transporting specimen-bearing microscope slides in an automated processing system, comprising:
sequentially moving a plurality of specimen-bearing microscope slides from a carrier to a slide staging device one at a time;
aligning an individual specimen-bearing microscope slide along a longitudinal axis at the slide staging device to an aligned position while the slide staging device contacts a lower surface of the individual specimen-bearing microscope slide;
contacting opposing lateral sides of the individual specimen-bearing microscope slide with aligning members of the slide staging device while the slide staging device contacts the lower surface of the individual specimen-bearing microscope slide;
moving a transfer head from a non-engaged position above the slide staging device to an engaged position adjacent to the individual specimen-bearing microscope slide;
capturing the individual specimen-bearing microscope slide using a capture feature of the transfer head; and
transporting the individual specimen-bearing microscope slide from the slide staging device to one or more specimen processing stations while the individual specimen-bearing microscope slide is in the aligned position.
18. The method of claim 17 , wherein moving the transfer head to the engaged position includes engaging a head alignment feature of the transfer head with at least one of a corresponding alignment feature of the slide staging device or a corresponding alignment feature of the one or more specimen processing stations, thereby aligning the transfer head with the slide staging device or the one or more specimen processing stations.
19. The method of claim 18 , wherein the head alignment feature comprises first and second elongate rods extending away from the transfer head, and the corresponding alignment feature on one of the slide staging device or the one or more specimen processing stations comprises first and second openings configured to receive the first and second elongate rods, respectively, as the transfer head moves to the engaged position.
20. The method of claim 17 , wherein:
the capture feature comprises a suction device including—
a sealing member positioned to contact the microscope slide when the transfer head is in the engaged position;
a vacuum port within the sealing member; and
a vacuum source fluidly connected to the vacuum port; and
the method further comprises drawing a vacuum by the vacuum source through the vacuum port to form an airtight seal between the sealing member and the microscope slide.