Compositions and methods to regulate Renalase in the treatment of cancer
The invention provides compositions and methods for binding and inhibiting renalase. In one embodiment, the renalase binding molecule inhibits renalase activity. Thus, in diseases and conditions where a reduction of renalase activity is beneficial, such inhibitory renalase binding molecules act as therapeutics.
1. A composition comprising an isolated monoclonal antibody, wherein the antibody is selected from the group consisting of:
a) an antibody comprising the heavy chain CDR1 sequence of SEQ ID NO:27; the heavy chain CDR2 sequence of SEQ ID NO:28; the heavy chain CDR3 sequence of SEQ ID NO:29; the light chain CDR1 sequence of SEQ ID NO: 30; the light chain CDR2 sequence of SEQ ID NO:31; and the light chain CDR3 sequence of SEQ ID NO:32; and
b) an antibody comprising the heavy chain CDR1 sequence of SEQ ID NO:43; the heavy chain CDR2 sequence of SEQ ID NO:44; the heavy chain CDR3 sequence of SEQ ID NO:45; the light chain CDR1 sequence of SEQ ID NO: 46; the light chain CDR2 sequence of SEQ ID NO:47; and the light chain CDR3 sequence of SEQ ID NO:48.
2. The composition of claim 1 , wherein the antibody specifically binds to renalase with an affinity of at least 10 −6 M.
3. The composition of claim 1 , wherein the antibody is selected from the group consisting of an immunoconjugate, a defucosylated antibody, and a bispecific antibody.
4. The composition of claim 3 , wherein the immunoconjugate comprises a therapeutic agent or a detection moiety.
5. The composition of claim 1 , wherein the antibody is selected from the group consisting of a humanized antibody, a chimeric antibody, a fully human antibody, and an antibody mimetic.
6. The composition of claim 1 , wherein the antibody of a) comprises the heavy chain sequence as set forth in SEQ ID NO:25.
7. The composition of claim 1 , wherein the antibody of a) comprises the light chain sequence as set forth in SEQ ID NO:26.
8. The composition of claim 1 , wherein the antibody of b) comprises the heavy chain sequence as set forth in SEQ ID NO:41.
9. The composition of claim 1 , wherein the antibody of b) comprises the light chain sequence as set forth in SEQ ID NO:42.