Antibody specific to spike protein of SARS-CoV-2 and uses thereof
The present disclosure relates to an antibody or antigen-binding fragment thereof that specifically binds to a spike protein of SARS-CoV-2. The present disclosure also relates to a pharmaceutical composition, a method for treating and/or preventing diseases and/or disorders caused by a coronavirus in a subject in need thereof, and a method for detecting a coronavirus in a sample.
1. A complex comprising an antibody or antigen-binding fragment thereof that is specific for an epitope in a spike protein of a CoV; wherein the epitope comprises a part locating in 419 to 433 amino acid residues or 471 to 482 amino acid residues of SEQ ID NO: 8,
wherein the antibody or antigen-binding fragment thereof comprises complementarity determining regions (CDRs) of a heavy chain variable region and complementarity determining regions of a light chain variable region, wherein the complementarity determining regions of the heavy chain variable region comprise CDRH1, CDRH2, and CDRH3 regions, and the complementarity determining regions of the light chain variable region comprise CDRL1, CDRL2, and CDRL3 regions,
wherein the CDRH1 region comprises the amino acid sequence of SEQ ID NO: 1; the CDRH2 region comprises the amino acid sequence of SEQ ID NO: 2; the CDRH3 region comprises the amino acid sequence of SEQ ID NO: 3; and
wherein the CDRL1 region comprises the amino acid sequence of SEQ ID NO: 4; the CDRL2 region comprises the amino acid sequence of WAS; the CDRL3 region comprises the amino acid sequence of SEQ ID NO: 5.
2. The complex of claim 1 , wherein the spike protein is fully glycosylated.
3. The complex of claim 1 , wherein the antibody or antigen-binding fragment thereof comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6; and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 7.
4. The complex of claim 1 , wherein the antibody is a monoclonal antibody, chimeric antibody, humanized antibody or human antibody.
5. The complex of claim 1 , wherein the antibody is multispecific.
6. The complex of claim 1 , wherein the CoV is alpha-CoV, beta-CoV, gamma-CoV, and delta-CoV2.
7. The complex of claim 1 , wherein the CoV is SARS-CoV, MERS-CoV or SARS-CoV-2.
8. A method for treating infection with a coronavirus in a subject in need thereof, comprising administering a therapeutically effective amount of the antibody or antigen-binding fragment thereof of claim 1 .
9. The method of claim 8 , wherein the method comprises injecting the antibody or antigen-binding fragment thereof into the body of the subject subcutaneously, intravenously, or intramuscularly.
10. The method of claim 8 , wherein the method neutralizes a coronavirus in the subject in need thereof.
11. A vector encoding the antibody or antigen-binding fragment thereof of claim 1 .
12. The vector of claim 11 , wherein the vector is a plasmid or a viral vector.