High affinity human antibodies to human IL-4 receptor
The present invention provides nucleic acid molecules that encode antibodies or antigen-binding fragments thereof, which specifically bind human interleukin-4 receptor (IL-4R). Also provided are expression vectors comprising nucleic acid molecule that encode anti-IL-4R antibodies, host cells comprising the expression vectors, and methods of producing anti-IL-4R antibodies or antigen-binding fragments thereof comprising growing the host cells under conditions permitting production of the antibody or fragment, and recovering the antibody or fragment so produced.
1. An antibody or antigen-binding fragment thereof, that specifically binds human interleukin-4 receptor (hIL-4R), comprising a heavy chain variable region (HCVR) and a light chain variable region (LCVR), wherein the antibody or antigen-binding fragment comprises
an HCVR that comprises a heavy chain complementarity determining region 1 (HCDR1) comprising the amino acid sequence of SEQ ID NO: 196, an HCDR2 comprising the amino acid sequence of SEQ ID NO: 198, and an HCDR3 comprising the amino acid sequence of SEQ ID NO: 200, and an LCVR that comprises a light chain complementarity determining region 1 (LCDR1) comprising the amino acid sequence of SEQ ID NO:204, an LCDR2 comprising the amino acid sequence of SEQ ID NO:206, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:208.
2. The antibody or antigen-binding fragment thereof of claim 1 , wherein the antibody or antigen-binding fragment comprises
an HCVR comprising the amino acid sequence of SEQ ID NO:210 and an LCVR comprising the amino acid sequence of SEQ ID NO:212.
3. An isolated nucleic acid molecule comprising a nucleic acid sequence encoding the antibody or antigen-binding fragment thereof of claim 1 .
4. A vector comprising the nucleic acid sequence-molecule of claim 3 .
5. An isolated host cell comprising the vector of claim 4 .
6. A method of producing an antibody or antigen-binding fragment thereof which specifically binds human interleukin-4 receptor alpha (hIL-4R), comprising growing the host cell of claim 5 under conditions in which the antibody or antigen-binding fragment is produced, and recovering the anti-hIL-4 antibody or antigen-binding fragment so produced.
7. The host cell of claim 5 , wherein the host cell is a mammalian cell.
8. The host cell of claim 5 , wherein the host cell is an E. coli cell or a CHO cell.
9. A method of treating a disease or disorder, wherein the disease or disorder is improved, ameliorated or inhibited by removal, inhibition or reduction of human interleukin-4 (hIL-4) activity, said method comprising administering the antibody or antigen-binding fragment of claim 1 to a patient having the disease or disorder.
10. The method of claim 9 , wherein the disease or disorder is selected from the group consisting of arthritis, herpetiformis, chronic idiopathic urticaria, scleroderma, hypertrophic scarring, Whipple's Disease, benign prostate hyperplasia, lung disorders, asthma, inflammatory disorders, allergic reactions, Kawasaki disease, sickle cell disease, Churg-Strauss syndrome, Grave's disease, pre-eclampsia, Sjogren's syndrome, autoimmune lymphoproliferative syndrome, autoimmune hemolytic anemia, Barrett's esophagus, autoimmune uveitis, tuberculosis, and nephrosis.
11. The method of claim 9 , wherein the disease or disorder is asthma.
12. The method of claim 9 , wherein the disease or disorder is atopic dermatitis.