Optically-induced auto-encapsulation
The present disclosure provides methods and systems for optically-induced auto-encapsulation. A method of the present disclosure comprises providing a media chamber comprising a medium comprising (i) a three-dimensional (3D) object comprising a photo-emitter, (ii) at least one polymeric precursor, and (iii) a photoinitiator, and subjecting the medium in the media chamber to a stimulus (e.g., an energy beam or a chemical stimulus) to induce photo-emission from the photo-emitter to trigger the formation of a polymer matrix from the at least one polymeric precursor, which polymer matrix at least partially encapsulates the 3D object.
1. A method for at least partially encapsulating a three-dimensional (3D) object, comprising
(a) providing a media chamber comprising a medium comprising (i) said 3D object comprising a photo-emitter, wherein said 3D object is a biological material, (ii) at least one polymeric precursor, and (iii) a photoinitiator; and
(b) directing at least one energy beam to said medium in said media chamber to induce photo-emission from said photo-emitter wherein said photo-emitter emits energy that triggers said photoinitiator to initiate formation of a polymer matrix from said at least one polymeric precursor, which polymer matrix at least partially encapsulates said 3D object.
2. The method of claim 1 , wherein said photo-emitter comprises one or more emissive components.
3. The method of claim 2 , wherein said photo-emitter is a fluorescent polymer sphere.
4. The method of claim 2 , wherein said photo-emitter is a cell-containing emissive nanoparticle.
5. The method of claim 1 , wherein said medium further comprises a photoinhibitor that prevents formation of said polymer matrix in one or more selected locations of said medium.
6. The method of claim 1 , wherein said at least one energy beam comprises a single wavelength.
7. The method of claim 1 , further comprising using a full spectrum illumination with distinct wavelength bands blocked.
8. The method of claim 1 , further comprising using a full spectrum illumination.
9. The method of claim 1 , wherein said at least one energy beam is selected from the group consisting of a laser, a light emitting diode, an arc lamp, an incandescent light source, and a fluorescent light source.
10. The method of claim 1 , wherein said 3D object comprises a cell.
11. The method of claim 10 , wherein said cell is selected from the group consisting of an endothelial cell, a fibroblast, an immune cell, a keratinocyte, a melanocyte, a kidney cell, a hepatocyte, and a cardiomyocyte.
12. The method of claim 1 , wherein said photo-emitter is selected from the group consisting of a chemiluminescent component, a photoluminescent component, a fluorescent molecule, a phosphorescent molecule, a luminescent molecule, a nanoparticle, a quantum dot particle, a microparticle, a histological stain, an immunofluorescent dye, a fluorescent dye, a fluorescent protein, a photo-emitting peptide, a photo-emitting cell penetrating peptide, a photo-emitting intracellular probe, a photo-emitting reporter, and a photo-emitting activity-based reporter.
13. The method of claim 1 , wherein said 3D object comprises at least two photo-emitters.
14. The method of claim 13 , wherein said 3D object comprises a fluorescent resonance energy transfer (FRET) reporter.
15. The method of claim 1 , wherein said photoinitiator is selected from the group consisting of Eosin Y, erythrosine, rose Bengal, xanthone derivatives, triethanolamine, azobisisobutyronitrile (AIBN), benzoin derivatives, benziketals, hydroxyalkylphenone, acetophenone derivatives, trimethylopropane triacrylate (TPT), acryloyl chloride, benzoyl peroxide, camphorquinone, benzophenone, thioxanthones, and 2-hydroxy-1-[4-(hydroxyethoxy)phenyl]-2-methyl-1-propanone, 4-(2-hydroxyethylethoxy)-phenyl-(2-hydoxy-2-methyl propyl) ketone, 1-hydroxycyclohexyl-1-phenyl ketone and 2,2-dimethoxy-2-phenylacetophenone, and lithium phenyl-2,4,6-trimethylbenzolphosphinate (LAP).
16. The method of claim 1 , wherein said at least one polymeric precursor is selected from the group consisting of collagen, gelatin, chitosan, polyethylene glycol (PEG), polylactic-co-glycolic acid (PLGA), vinyl alcohol derivatives, vinylpyrrolidone derivatives, acrylate derivatives, methacrylate derivatives, and styrene derivatives.
17. The method of claim 1 , wherein said media chamber further comprises a second three-dimensional (3D) object, wherein said second 3D object does not contain a photo-emitter.
18. The method of claim 1 , wherein said medium comprises more than one 3D object comprising a photo-emitter.
19. The method of claim 18 , wherein said more than one 3D object comprises a photo-emitter that does not have overlapping excitation wavelengths with another 3D object comprising a photo-emitter.
20. The method of claim 1 , further comprising differentiating different 3D object types based on the formation of said polymer matrix from said at least one polymeric precursor.