Hemagglutinin complex protein and application thereof
The present invention provides a miniaturized hemagglutinin complex protein having function inhibitory activity against E-cadherin, wherein (a) all or a part of at least HA1 subcomponent, and/or a part of HA3 subcomponent are/is deleted, (b) regions in HA2 and HA3 subcomponents that contribute to binding with E-cadherin are present, and (c) the HA3 subcomponent is derived from Clostridium botulinum type A or type B, and an E-cadherin function inhibitor containing the hemagglutinin complex protein.
1. A miniaturized hemagglutinin complex protein having function inhibitory activity against E-cadherin, wherein the miniaturized hemagglutinin complex protein is selected from the group consisting of:
(1) mini-HA consisting of HA1 subcomponent of SEQ ID NO:10, HA2 subcomponent of SEQ ID NO: 4, and HA3 subcomponent fragment of SEQ ID NO:2,
(2) FL (2+3)-C consisting of HA2 subcomponent of SEQ ID NO:4 and HA3 component with Strep-Tag (C-terminus) of SEQ ID NO:44,
(3) linked-mini (2+3)/BB of SEQ ID NO:47,
(4) linked-mini (2+3)/CB of SEQ ID NO:48,
(5) linked-mini (2+3)/CB-YFDY of SEQ ID NO:49, and
(6) linked-mini (2+3)/CB-LD/YFDY of SEQ ID NO:50.
2. An E-cadherin function inhibitor comprising the hemagglutinin complex protein according to claim 1 .
3. The inhibitor according to claim 2 , wherein the function of E-cadherin is a cell-cell adhesion function.
4. A method for inhibiting cell-cell adhesion in a cell population comprising bringing the hemagglutinin complex protein according to claim 1 into contact with the cell population.
5. The method according to claim 4 , wherein the cell population is a population of pluripotent stem cells.
6. A cell proliferation promoter comprising the hemagglutinin complex protein according to claim 1 .
7. A method for promoting proliferation of a cell comprising culturing the cell in the presence of the hemagglutinin complex protein according to claim 1 .
8. The miniaturized hemagglutinin complex protein according to claim 1 , wherein the miniaturized hemagglutinin complex protein is selected from the group consisting of:
(1) linked-mini (2+3)/BB of SEQ ID NO:47,
(2) linked-mini (2+3)/CB of SEQ ID NO:48,
(3) linked-mini (2+3)/CB-YFDY of SEQ ID NO:49, and
(4) linked-mini (2+3)/CB-LD/YFDY of SEQ ID NO:50.