IP Library › Granted Patent US 12,195,727
Granted Patent B2
US 12,195,727 · App. 17/627,789 · Granted Jan 14, 2025

Patent

Inventors: Tongda Li (Southbank, AU); Ian Ross Tannenbaum (Bundoora, AU); Jatinder Kaur (Taylors Hill, AU); Christian Krill (Reservoir, AU); Timothy Ivor Sawbridge (Coburg, AU); Ross Mann (Coburg, AU); German Carlos Spangenberg (Bundoora, AU)
Assignees: Agriculture Victoria Services PTY LTD; Dairy Australia Limited; Geoffrey Gardiner Dairy Foundation Limited
C12N1/205A01H3/00A01H17/00A01N63/27C12R2001/38
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Quick Facts
Patent No.
US 12,195,727
App. No.
17/627,789
Granted
Jan 14, 2025
Kind
B2
Abstract

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Pseudomonas poae which provides bioprotection and/or biofertilizer phenotypes to plants into which it is inoculated. The present invention also relates to plants infected with the endophyte and related methods.

Claims (29)

1. A purified or isolated endophyte strain, wherein said endophyte is a strain of Pseudomonas poae which provides bioprotection and/or biofertilizer phenotypes to plants into which it is inoculated and is Pseudomonas poae strain EY as deposited with The National Measurement Institute on 17th May 2019 with accession number V19/009907.

2. The endophyte according to claim 1 , wherein the bioprotection and/or biofertilizer phenotype includes production of a bioprotectant compound in the plant into which the endophyte is inoculated.

3. The endophyte according to claim 2 , wherein the bioprotectant compound is poaeamide or a derivative, isomer and/or salt thereof.

4. The endophyte according to claim 1 , wherein the bioprotection and/or biofertilizer phenotype is selected from the group consisting of production of organic acids, solubilisation of phosphate and nitrogen fixation in the plant into which the endophyte is inoculated.

5. A plant or part thereof inoculated with one or more endophytes according to claim 1 , wherein the plant into which the endophyte is inoculated is an endophyte-free host plant or part thereof and is stably infected with said endophyte.

6. A method for producing a bioprotectant compound, or a derivative, isomer and/or a salt thereof, said method including infecting a plant with the endophyte according to claim 1 and cultivating the plant under conditions suitable to produce the bioprotectant compound; or

said method including culturing the endophyte according to claim 1 under conditions suitable to produce the bioprotectant compound; and

optionally isolating the bioprotectant compound from the plant or culture medium.

7. The method according to claim 6 , wherein the conditions include a culture medium including a source of carbohydrates.

8. A method of providing bioprotection to a plant against bacterial and/or fungal pathogens and/or providing biofertilizer to a plant, said method including infecting the plant with the endophyte according to claim 1 and cultivating the plant.

9. The method according to claim 8 , wherein the method includes providing bioprotection to the plant and includes production of a bioprotectant compound in the plant into which the endophyte is inoculated; or

wherein the method includes providing biofertilizer to the plant and includes production of organic acids, increased phosphate use efficiency, increased solubilisation of phosphate, increased nitrogen use efficiency and/or increased nitrogen availability, in the plant into which the endophyte is inoculated; or

wherein the method includes increasing phosphate use efficiency or increasing phosphate solubilisation in the plant, and wherein the plant is cultivated in the presence of soil phosphate and/or applied phosphate; or

wherein the method includes increasing nitrogen use efficiency or nitrogen availability, and

wherein the plant is cultivated in a low nitrogen medium.

10. A method of increasing phosphate use efficiency or increasing phosphate solubilisation by a plant, said method including infecting the plant with the endophyte according to claim 1 , and cultivating the plant.

11. The method according to claim 10 , wherein the plant is cultivated in the presence of soil phosphate and/or applied phosphate.

12. A method of growing a plant in a low nitrogen medium, said method including infecting the plant with the bioprotectant compound-producing endophyte according to claim 1 , and cultivating the plant.

13. The method according to claim 12 , wherein the plant is cultivated in soil.

14. The plant according to claim 5 which is an agricultural plant species selected from one or more of forage grass, turf grass, bioenergy grass, grain crop and industrial crop.

15. The plant according to claim 5 which is selected from the group consisting of:

a forage, turf or bioenergy grass selected from the group consisting of those belonging to the genera Lolium and Festuca , including L. perenne (perennial ryegrass), L. arundinaceum (tall fescue) and L. multiflorum (Italian ryegrass), and those belonging to the Brachiaria - Urochloa species complex (panic grasses), including Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria stolonifera, Brachiaria ruziziensis, B. dictyoneura, Urochloa brizantha, Urochloa decumbens, Urochloa humidicola, Urochloa mosambicensis and interspecific and intraspecific hybrids of Brachiaria - Urochloa species complex; or

is a grain crop or industrial crop selected from the group consisting of those belonging to the genus Triticum , including T. aestivum (wheat), those belonging to the genus Hordeum , including H. vulgare (barley), those belonging to the genus Avena , including A. sativa (oats), those belonging to the genus Zea , including Z. mays (maize or corn), those belonging to the genus Oryza , including O. sativa (rice), those belonging to the genus Saccharum including S. officinarum (sugarcane), those belonging to the genus Sorghum including S. bicolor (sorghum), those belonging to the genus Panicum , including P. virgatum (switchgrass), those belonging to the genera Miscanthus, Paspalum, Pennisetum, Poa, Eragrostis and Agrostis ; or

is a grain crop or industrial crop selected from the group consisting of wheat, barley, oats, chickpeas, triticale, fava beans, lupins, field peas, canola, cereal rye, vetch, lentils, millet/panicum, safflower, linseed, sorghum, sunflower, maize, canola, mungbeans, soybeans, and cotton.

16. The method of claim 7 , wherein the source of carbohydrates is selected from one or more of the group consisting of a starch/sugar-based agar or broth, a cereal-based agar or broth, endophyte agar, Murashige and Skoog with 20% sucrose, half V8 juice/half PDA, water agar and yeast malt extract agar.

17. The method of claim 9 , wherein the bioprotectant compound is poaeamide or a derivative, isomer and/or salt thereof.

18. The method of claim 9 , wherein the applied phosphate includes phosphate applied by fertilizer.

19. The method of claim 9 , wherein the low nitrogen medium is low nitrogen soil.

20. The method of claim 11 , wherein the applied phosphate includes phosphate applied by fertilizer and wherein the plant is cultivated in soil.

Priority Claims (1)
AU 2019902560 · Jul 19, 2019 · national
Continuity (1)
Related Publication 20230193193A1 · Jun 22, 2023
References Cited (43)
US 20200178540A1 · Dagher et al. · 2020 [cited by applicant]
CN 108467839A · 2018 [cited by applicant]
CN 109566655A · 2019 [cited by applicant]
WO 2019023226A1 · 2019 [cited by applicant]
Vyas et al. BMC Microbiol 9, 174 (2009). https://doi.org/10.1186/1471-2180-9-174. [cited by examiner]
Gupta et al. Sustainable Agriculture Reviews. Sustainable Agriculture Reviews, vol. 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5449-2_8 Oct. 25, 2012. [cited by examiner]
Behrendt et al. International Journal of Systemic and Evolutionary Microbiology (2003), 53, 1461-1469. [cited by examiner]
Nutrient Broth II retrieved from http://biotrading.com/assets/productinformatie/sifin/tds/tn1174-pi-en_2016_06_21_15_25.pdf dated Mar. 20, 2014. [cited by examiner]
Behrendt, U. et al. “Fluorescent pseudomonads associated with the phyllosphere of grasses; [cited by applicant]
Cho, K. et al. Endophytic bacterial communities in Ginseng and their Antifungal Activity Against Pathogens Microbial Ecology, 2007, pp. 341-351, vol. 54, No. 2. [cited by applicant]
Ghosh, R. et al. “Biological control of Alternaria alternata causing leaf spot disease of Aloe Vera using two strains of rhizobacteria”, Biological Control, 2016, pp. 102-108, vol. 97. [cited by applicant]
Irshad, U. et al. “Bacterial Subspecies Variation and Nematode Grazing Change P Dynamics in the Wheat Rhizosphere”, Frontiers in Microbiology, 1990, pp. 1-11, vol. 9. [cited by applicant]
Verma, P. et al. “Appraisal of diversity and functional attribute of thermotolerant wheat associated bacteria from the peninsular zone of India”, Saudi Journal of Biological Sciences, 2016, pp. 1882-1895, vol. 26, No. 7. [cited by applicant]
Vyas, P. et al. “Organic acid production in vitro and plant growth promotion in maize underphosphate-solubilizing fluorescent Pseudomonas”, BMC Microbiology, 2009, pp. 1-15, vol. 9, No. 174. [cited by applicant]
Ankenbrand, M. et al. “ AliTV-interactive visualization of whole genome comparisons”, PeerJ Comput. Sci., 2017, pp. 1-11, vol. 3. [cited by applicant]
Bolger, A. et al. “Trimmomatic: a flexible trimmer for Illumina sequence data”, Bioinformatics, 2014, pp. 2114-2120, vol. 30, No. 15. [cited by applicant]
Chun, J. et al. “Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes”, International Journal of Systematic and Evolutionary Microbiology, 2018, pp. 461-466, vol. 68. [cited by applicant]
De Coster, W. et al. “NanoPack: visualizing and processing long-read sequencing data”, Bioinformatics, 2018, pp. 2666-2669, vol. 34, No. 15. [cited by applicant]
Henry, E. et al. “Direct and Indirect Visualization of Bacterial Effector Delivery into Diverse Plant Cell Types during Infection”, The Plant Cell, 2017, pp. 1555-1570, vol. 29. [cited by applicant]
Li, H. et al. “Minimap2: pairwise alignment for nucleotide sequences”, Bioinformatics, 2018, pp. 3094-3100, vol. 34, No. 18. [cited by applicant]
Li, Q. et al., GENBANK Accession No. MG835948.1, Pseudomonas poae strain HTM601-1 16S ribosomal RNA gene, partial sequence, Feb. 9, 2019. [cited by applicant]
Li, Q. et al., GENBANK Accession No. MG835959.1, Pseudomonas poae strain HTM603-3 16S ribosomal RNA gene, partial sequence, Feb. 9, 2019. [cited by applicant]
Li, Q. et al., GENBANK Accession No. MG835996.1, Pseudomonas poae strain HTI604-2 16S ribosomal RNA gene, partial sequence, Feb. 9, 2019. [cited by applicant]
Li, Q. et al., GENBANK Accession No. MG836002.1, Pseudomonas poae strain HTI605-4 16S ribosomal RNA gene, partial sequence, Feb. 9, 2019. [cited by applicant]
Lindeberg, M. et al. “Pseudomonas syringae type III effector repertoires: last words in endless arguments”, Cell Press, 2012, pp. 199-208, vol. 20, No. 4. [cited by applicant]
Löytynoja, A. et al. “Phylogeny-aware alignment with PRANK”, Multiple Sequence Alignment Methods, Methods in Molecular Biology, pp. 155-170, vol. 1079, 2014. [cited by applicant]
Müller, H. et al. “Complete Genome Sequence of the Sugar Beet Endophyte Pseudomonas poae RE*1-1-14, a disease-Suppressive Bacterium”, Genome Announcements, 2013, pp. 1-2, vol. 1, Issue 2. [cited by applicant]
Page, A. et al. “Roary: rapid large-scale prokaryote pan genome analysis”, Bioinformatics, 2015, pp. 3691-3693, vol. 31, No. 22. [cited by applicant]
Price, M. et al. FastTree 2—Approximately Maximum-Likilihood Trees for Large Alignments, PLOS One, Mar. 2010, pp. 1-10, vol. 5, Issue 3. [cited by applicant]
Sundara Rao, W.V.B. et al. “Phosphate Dissolving Micro-Organisms in the Soil and Rhizosphere”, Indian Agricultural Research Institute, 1963, pp. 272-278, vol. 33, No. 7. [cited by applicant]
Richter, M. et al. “Shifting the genomic gold standard for the prokaryotic species definition”, PNAS, 2009, pp. 19126-19131, vol. 106, No. 45. [cited by applicant]
Rodiguez, H. et al. “Phosphate solubilizing bacteria and their role in plant growth promotion”, Biotechnolgy Advances, 1999, pp. 319-339, vol. 17. [cited by applicant]
Seemann, T. et al. “Prokka: rapid prokaryotic genome annotation”, Bioinformatics, 2014, pp. 2068-2069, vol. 30, No. 14. [cited by applicant]
Sharma, S. et al. “Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils” SpringerPlus, 2013, pp. 1-14, vol. 2, No. 587. [cited by applicant]
Sun, H. et al. “Benefits of Pseudomonas poae s61 on Astragalus mongholicus growth and bioactive compund accumulation under drought stress”, Journal of Plant Interactions, 2019, pp. 205-212, vol. 14, No. 1. [cited by applicant]
Tallapragada, J. et al. “Phosphate-solubilizing microbes and their occurrence in the rhizospheres of Piper betel in Karnataka, India”, Turk. J. Biol., 2012, pp. 25-35, vol. 36. [cited by applicant]
Vaser, R. et al. “Fast and accurate de novo genome assembly from long uncorrected reads”, Genome Research, 2017, pp. 737-746, vol. 27. [cited by applicant]
Walker, B. et al. “Pilon: An integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement”, PLOS One, 2014, pp. 1-14, vol. 9, Issue 11. [cited by applicant]
Weber, T. et al. “antiSMASH 3.0—a comprehensive resource for the genome mining of biosynthetic gene clusters”, Nucleic Acids Research, 2015, pp. W237-W243, vol. 43. [cited by applicant]
Wei, H. et al. “Modular Study of the Type III Effector Repertoire in [cited by applicant]
Wick, R. et al. “Bandage: interactive visualization of de novo genome assemblies” Bioinformatics, 2015, pp. 3350-3352, vol. 31, No. 20. [cited by applicant]
Wick, R. et al. “Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads”, PLOS Computational Biology, 2017, pp. 1-22, vol. 13, No. 6. [cited by applicant]
Zachow, C. et al. “The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 is involved in Pathogen Suppression and Root Colonization” Molecular Plant-Microbe Interactions, 2015, pp. 800-810, vol. 28,… [cited by applicant]
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