Cyclooctatetraene containing dyes and compositions
Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.
1. A composition for preventing or reducing photobleaching of a fluorescent dye, comprising cyclooctatetraene or an optionally substituted derivative thereof, and one or more antioxidants, wherein the one or more antioxidants comprises quercetin or an optionally substituted derivative thereof, and wherein the composition is an aqueous solution.
2. The composition of claim 1 , wherein the fluorescent dye is excitable by a light source having a wavelength from about 350 nm to about 500 nm, from about 420 nm to about 480 nm, or from about 450 nm to about 460 nm.
3. The composition of claim 1 , wherein the concentration of cyclooctatetraene or the optionally substituted derivative in the composition is from about 0.1 mM to about 100 mM.
4. The composition of claim 3 , wherein the concentration of cyclooctatetraene or the optionally substituted derivative in the composition is from about 1 mM to about 20 mM, or about 10 mM.
5. The composition of claim 3 , wherein the concentration of quercetin or the optionally substituted derivative in the composition is from about 0.01 mM to about 10 mM.
6. The composition of claim 5 , wherein the concentration of quercetin or the optionally substituted derivative in the composition is from about 0.05 mM to about 2 mM, or about 0.25 mM.
7. The composition of claim 1 , further comprising ascorbic acid or a salt thereof.
8. A method of protecting a labeled nucleotide from light-induced degradation, comprising contacting the labeled nucleotide with the composition of claim 1 .
9. The method of claim 8 , wherein the labeled nucleotide is incorporated into an oligonucleotide or polynucleotide.
10. A method of reducing or preventing the light-induced degradation of nucleic acids during a nucleic acid sequencing reaction, comprising:
(a) contacting a solid support with an incorporation mixture comprising DNA polymerase and one or more of four different types of nucleotides, wherein the solid support comprises a plurality of immobilized target polynucleotides and sequencing primers that are complementary and hybridized to at least a portion of the target polynucleotides;
(b) incorporating one type of nucleotides into sequencing primers to produce extended copy polynucleotide strands, wherein each type of nucleotides comprises a 3′ blocking group, and one or more of the four different types of nucleotides comprises a fluorescent label;
(c) performing one or more fluorescent measurements to determine the identity of the incorporated nucleotides in a first buffer composition;
(d) chemically removing the 3′ blocking group of the incorporated nucleotides; and
(e) washing the extended copy polynucleotide strands with a wash solution;
wherein the first buffer composition comprises cyclooctatetraene or an optionally substituted derivative thereof, and one or more antioxidants selected from the group consisting of taxifolin, quercetin, allyl thiourea, dimethyl thiourea, silibinin, and trolox, and optionally substituted derivatives and combinations thereof.
11. The method of claim 10 , wherein the first buffer composition comprises cyclooctatetraene and quercetin, or optionally substituted derivatives thereof.
12. The method of claim 11 , wherein the concentration of cyclooctatetraene or the optionally substituted derivative in the first buffer composition is from about 0.1 mM to about 100 mM, from about 1 mM to about 20 mM, or about 10 mM.
13. The method of claim 11 , wherein the concentration of quercetin or the optionally substituted derivative in the first buffer composition is from about 0.01 mM to about 10mM, from about 0.05 mM to about 2 mM, or about 0.25 mM.
14. The method of claim 10 , wherein step (d) also removes the fluorescent label of the incorporated nucleotides.
15. The method of claim 10 , wherein steps (a) to (e) are repeated at least 50 times.
16. The method of claim 10 , wherein step (c) comprises irradiating the extended copy polynucleotide strands in the first buffer composition with a light source having a wavelength from about 400 nm to about 500 nm.
17. The method of claim 10 , wherein step (d) comprises contacting the extended copy polynucleotide strands with a phosphine.
18. The method of claim 17 , wherein the phosphine is tris (hydroxymethyl) phosphine, tris (hydroxyethyl) phosphine, or tris (hydroxypropyl) phosphine.
19. The method of claim 10 , wherein the first buffer composition further comprises ascorbic acid or a salt thereof.
20. A kit for use with a sequencing apparatus, comprising a plurality of chambers containing a plurality of compositions, wherein each chamber contains a single composition, wherein the plurality of compositions comprise:
incorporation reagents comprising one or more of four different types of 3′ blocked nucleotides and DNA polymerase;
a first buffer composition comprising cyclooctatetraene or an optionally substituted derivative thereof, and one or more antioxidants selected from the group consisting of taxifolin, quercetin, allyl thiourea, dimethyl thiourea, silibinin, trolox, and optionally substituted derivatives and combinations thereof;
cleavage reagents for chemically removing the 3′ blocking group of the one or more of four different types of nucleotides; and
a wash solution.
21. The kit of claim 20 , wherein the first buffer composition comprises cyclooctatetraene and quercetin, or optionally substituted derivatives thereof.
22. The kit of claim 21 , wherein the concentration of cyclooctatetraene or the optionally substituted derivative in the first buffer composition is from about 0.1 mM to about 100mM, from about 1 mM to about 20 mM, or about 10 mM.
23. The kit of claim 21 , wherein the concentration of quercetin or the optionally substituted derivative in the first buffer composition is from about 0.01 mM to about 10 mM, from about 0.05 mM to about 2 mM, or about 0.25 mM.
24. The kit of claim 20 , wherein the first buffer composition further comprises ascorbic acid or a salt thereof.