Granted Patent B2

US 12,201,422 · App. 17/128,287 · Granted Jan 21, 2025

Inventors: Joseph J. Vidalis (Bethesda, MD); Joseph A. Marcanio (Greensburg, PA); John Frederick Currie (Bethesda, MD); Helena Woodvine Snyder (Washington, DC); Vikas Bhatia (Gaithersburg, MD)

Assignee: Cambridge Medical Technologies LLC

A61B5/150076A61B5/14532A61B5/1486A61B5/150022A61B5/150274A61B5/150862A61B5/15134A61B5/157

Abstract

Claims (36)

1. A method of making a transdermal sampling and analysis device adapted to be placed on skin, the method comprising:

forming a base structure with at least a first electrode and a plurality of channel support structures;

forming a lid structure configured to be positioned above the at least first electrode, wherein forming the lid structure comprises:

depositing a conductive material layer on a lid substrate to create a lid conductive material layer; and

patterning the lid conductive material layer to form at least a second electrode; and

attaching the lid structure to the base structure using an adhesive material on surfaces of the plurality of channel support structures,

wherein attaching the lid structure to the base structure forms a plurality of channels in a reservoir between the plurality of channel support structures,

wherein the reservoir comprises:

a circular collection portion configured to collect a biological sample obtained through permeable skin barrier cells, wherein the circular collection portion is substantially parallel to a plane of the skin; and

a sensing chamber portion configured to receive the biological sample from the circular collection portion, wherein the sensing chamber portion surrounds a periphery of the circular collection portion substantially parallel to the plane of the skin, wherein the at least first electrode and the at least second electrode are located proximate to the sensing chamber portion.

2. The method of claim 1 , wherein forming the base structure comprises:

depositing a conductive material layer on a base substrate to create a base conductive material layer;

patterning the base conductive material layer to form a disruptor, the at least first electrode, and interconnects;

applying a photoresist material to the base substrate;

patterning the photoresist material to form the plurality of channel support structures; and

depositing a layer of an analyte sensing reagent on surfaces of the at least first electrode.

3. The method of claim 1 , wherein forming the lid structure further comprises:

applying a layer of an analyte sensing reagent to surfaces of the at least second electrode; and

applying a photoresist material to the lid substrate.

4. The method of claim 1 , wherein the adhesive material is approximately 5-20 μm thick.

5. A method of making a transdermal sampling and analysis device, comprising:

forming a base structure with at least a first electrode;

forming a lid structure configured to be positioned above the at least first electrode, wherein the lid structure has a plurality of channel support structures; and

attaching the lid structure to the base structure using an adhesive material on surfaces of the plurality of channel support structures,

wherein attaching the lid structure to the base structure forms a plurality of channels in a reservoir between the plurality of channel support structures,

wherein the reservoir comprises:

a circular collection portion configured to collect a biological sample obtained through permeable skin barrier cells, wherein the circular collection portion is substantially parallel to a plane of the skin; and

a sensing chamber portion configured to receive the biological sample from the circular collection portion, wherein the sensing chamber portion surrounds a periphery of the circular collection portion substantially parallel to the plane of the skin, wherein the at least first electrode is located proximate to the sensing chamber portion.

6. The method of claim 5 , wherein forming the base structure comprises:

depositing a conductive material layer on a base substrate to create a first conductive material layer;

patterning the first conductive material layer to form a disruptor, the at least first electrode, and interconnects; and

depositing a layer of an analyte sensing reagent on surfaces of the at least first electrode.

7. The method of claim 5 , wherein forming the lid structure comprises:

applying a photoresist material to the lid substrate;

patterning the photoresist material to form the plurality of channel support structures; and

selectively applying the adhesive material to surfaces of the plurality of channel support structures, wherein each surface comprises an outward-facing surface that directly contacts the base structure.

Assignments (3)

Continuity (3)

References Cited (151)