Honeycomb tube
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.
1. A reaction vessel comprising:
a planar frame elongated along a length that extends between opposite ends of the planar frame, the length being greater than a width of the planar frame;
a fluidic interface disposed at one end of the opposing ends of the planar frame, wherein the fluidic interface comprises a fluidic inlet and a fluidic outlet,
a fluidic path defined in the planar frame that fluidically connects the fluidic inlet to the fluidic outlet, and wherein the fluidic path comprises an inlet passage, an outlet passage and a well chamber, wherein the inlet passage is fluidically coupled between the fluidic inlet and the well chamber and the outlet passage is fluidically coupled between the fluidic outlet and the well chamber, and wherein the inlet passage and the outlet passage are located between the well chamber and the fluidic interface; and
a plurality of wells defined within the well chamber, wherein the plurality of wells are arranged in a well array.
2. The reaction vessel of claim 1 , wherein the planar frame is configured to be vertically oriented so that a plane along which the fluidic path and the well array extends is vertically oriented with the fluidic outlet positioned above the fluidic inlet, and the outlet passage positioned above the inlet passage.
3. The reaction vessel of claim 1 , wherein the outlet passage positioned above the inlet passage, and a first channel portion of the outlet passage extending from the well chamber is angled obliquely relative to vertical and horizontal directions.
4. The reaction vessel of claim 1 , wherein the fluidic path further comprises:
a pre-amplification chamber fluidically coupled between the inlet passage and the well chamber, wherein the pre-amplification chamber is a portion of the fluidic path having a lateral dimension greater than a diameter of the inlet and outlet passages and is configured for a fluid sample to be introduced via the inlet passage to undergo amplification before filling the plurality of wells in the well chamber.
5. The reaction vessel of claim 4 , wherein the pre-amplification chamber comprises a pre-amplification chamber exit fluidically connected with a well chamber entrance that is fluidically connected with the well chamber.
6. The reaction vessel of claim 5 , wherein the planar frame is configured to be vertically oriented so that a plane along which the fluidic path and well array extends is vertically oriented with the fluidic outlet positioned above the fluidic inlet and the outlet passage positioned above the inlet passage, and
wherein, with the planar frame vertically oriented, the well chamber entrance is positioned at a lower-most portion of the well chamber.
7. The reaction vessel of claim 6 , wherein with the body planar frame vertically oriented the pre-amplification chamber exit is positioned at an upper-most portion of the pre-amplification chamber.
8. The reaction vessel of claim 7 , wherein, with the body planar frame vertically oriented, the well chamber entrance is positioned lower than the pre-amplification chamber exit.
9. The reaction vessel of claim 8 , wherein the fluidic path comprises a passage fluidically connecting the pre-amplification chamber exit to the well chamber entrance, and
wherein, with the planar frame vertically oriented, the passage is arranged to slope downward from the pre-amplification chamber exit to the well chamber entrance.
10. The reaction vessel of claim 1 , wherein the planar frame defines an oil chamber configured to hold a hydrophobic substance, wherein the oil chamber is a portion of the fluidic path that is fluidically connected with the well chamber and having a lateral diameter greater than a diameter of the inlet and outlet passages and is configured such that a pressurized flow of a fluid along the fluidic path supplies the hydrophobic substance to the well chamber from the oil chamber.
11. The reaction vessel of claim 1 , wherein the inlet passage and the outlet passage extend parallel to each other between the well chamber and the fluidic interface.
12. The reaction vessel of claim 1 , wherein the planar frame comprises a first substrate and a second substrate, and
wherein the fluid path is defined between the first substrate and the second substrate by the frame.
13. The reaction vessel of claim 12 , wherein the first substrate comprises a first film sealed to the frame, and
wherein the second substrate comprises a second film sealed to the frame.
14. The reaction vessel of claim 12 , wherein one of the first substrate or the second substrate are integrally formed with the frame.
15. The reaction vessel of claim 14 , wherein the fluidic interface is integrally formed with the frame.
16. The reaction vessel of claim 1 , wherein the plurality of wells includes between 100 and 1,500 wells.
17. The reaction vessel of claim 1 , wherein the plurality of wells each have a diameter within a range from 50 to 500 μm.
18. The reaction vessel of claim 1 , wherein the plurality of wells comprises a plurality of nanowells each having a depth from 25 μm to 1000 μm.
19. The reaction vessel of claim 1 , wherein the fluidic interface is integrally formed with the frame.
20. The reaction vessel of claim 1 , wherein the fluidic interface is configured to be coupled to a liquid sample cartridge so as to fluidically couple the fluidic inlet and the fluidic outlet with corresponding fluid ports of the liquid sample cartridge.
21. A reaction vessel comprising:
a planar frame that is elongated along a length that extends between opposite ends of the planar frame, the length being greater than a width of the planar frame;
a fluidic interface disposed at one end of the opposing ends of the planar frame, wherein the fluidic interface comprises a fluidic inlet and a fluidic outlet,
a fluidic path defined in the planar frame that fluidically connects the fluidic inlet to the fluidic outlet, and wherein the fluidic path comprises an inlet passage, an outlet passage and a well chamber, wherein the inlet passage is fluidically coupled between the fluidic inlet and the well chamber and the outlet passage is fluidically coupled between the fluidic outlet and the well chamber, and wherein the inlet passage and the outlet passage are located between the well chamber and the fluidic interface; and
a plurality of wells defined within the well chamber.
22. The reaction vessel of claim 21 , wherein the plurality of wells includes between 100 and 1,500 wells.
23. The reaction vessel of claim 21 , wherein the plurality of wells each have a diameter within a range from 50 to 500 μm.
24. The reaction vessel of claim 21 , wherein the plurality of wells comprises a plurality of nanowells each having a depth from 25 μm to 1000 μm.
25. The reaction vessel of claim 21 , wherein the fluidic interface is configured to be coupled to a liquid sample cartridge so as to fluidically couple the fluidic inlet and the fluidic outlet with corresponding fluid ports of the liquid sample cartridge.