Virus lysis and nucleic acid preservation solution
A virus lysis and nucleic acid preservation solution is disclosed, including the following components: isopropanol or ethyl alcohol, guanidinium chloride, tri (hydroxymethyl) aminomethane hydrochloride, and ethylenediaminetetraacetic acid. Furthermore, a kit including the virus lysis and nucleic acid preservation solution, a method for preserving viral nucleic acid, and a method for extracting a viral nucleic acid are also provided. The virus lysis and nucleic acid preservation solution of the present invention has the functions of lysing virus and preserving nucleic acid in the lysed virus samples, and it is compatible with lysis buffers, wash buffers, elution buffers, etc. in all kinds of viral nucleic acid extraction kit commonly available in the market.
1. A virus lysis and nucleic acid preservation solution, comprising the following components:
isopropanol or ethyl alcohol,
guanidine hydrochloride,
tris (hydroxymethyl) aminomethane hydrochloride,
ethylenediaminetetraacetic acid,
proteinase K and
magnetic beads, wherein a concentration of the guanidine hydrochloride is 5-6 mol/L, a concentration of the tris (hydroxymethyl) aminomethane hydrochloride is 10-100 mmol/L, a concentration of the ethylenediaminetetraacetic acid is 2-5 mmol/L, and a volume percentage of the isopropanol or ethyl alcohol is 5%-40%, wherein the magnetic beads are hydroxyl magnetic beads, and wherein the guanidine hydrochloride in the virus lysis and nucleic acid preservation solution does not cause proteinase K inactivation or aggregation of the magnetic beads.
2. The virus lysis and nucleic acid preservation solution according to claim 1 , wherein in the virus lysis and nucleic acid preservation solution, the concentration of the guanidine hydrochloride is 5 mol/L, the concentration of the tris (hydroxymethyl) aminomethane hydrochloride is 50-80 mmol/L, and the concentration of the ethylenediaminetetraacetic acid is 2.5-4 mmol/L, and the volume percentage of the isopropanol or ethanol is 10%-30%.
3. The virus lysis and nucleic acid preservation solution according to claim 1 , wherein a pH of the virus lysis and nucleic acid preservation solution is 6-9.
4. The virus lysis and nucleic acid preservation solution according to claim 3 , wherein a pH of the virus lysis and nucleic acid preservation solution is 7-8.
5. The virus lysis and nucleic acid preservation solution according to claim 1 , wherein the virus lysis and nucleic acid preservation solution is prepared with sterile water as a solvent.
6. A kit, comprising the virus lysis and nucleic acid preservation solution according to claim 1 , wherein the kit is configured for a virus preservation and/or a nucleic acid extraction.
7. A method for preserving viral nucleic acid comprising a step of adding the solution according to claim 1 to a sample containing a virus.
8. The virus lysis and nucleic acid preservation solution according to claim 2 , wherein a pH of the virus lysis and nucleic acid preservation solution is 6-9.
9. The virus lysis and nucleic acid preservation solution according to claim 2 , wherein the virus lysis and nucleic acid preservation solution is prepared with sterile water as a solvent.
10. The kit according to claim 6 , wherein in the virus lysis and nucleic acid preservation solution, the concentration of the guanidine hydrochloride is 5 mol/L, the concentration of the tris (hydroxymethyl) aminomethane hydrochloride is 50-80 mmol/L, the concentration of the ethylenediaminetetraacetic acid is 2.5-4 mmol/L, and the volume percentage of the isopropanol or ethanol is 10%-30%.
11. The kit according to claim 6 , wherein a pH of the virus lysis and nucleic acid preservation solution is 6-9.
12. The kit according to claim 11 , wherein a pH of the virus lysis and nucleic acid preservation solution is 7-8.
13. The kit according to claim 6 , wherein the virus lysis and nucleic acid preservation solution is prepared with sterile water as a solvent.