Granted Patent B2

US 12,226,487 · App. 18/628,440 · Granted Feb 18, 2025

Inventors: Kristian Sass-Ørum (Bagsvaerd, DK); Rasmus Jørgensen (Søborg, DK); Sebastian Beck Jørgensen (Bagsværd, DK); Henning Thøgersen (Bagsværd, DK); Thomas Hoeg-Jensen (Bagsværd, DK); Michael Paolo Bastner Sandrini (Bagsværd, DK)

Assignee: Cytoki Pharma ApS

A61K47/542A61P1/16A61P3/04A61P3/10A61P11/00

Abstract

Claims (12)

1. A derivative of IL-22 comprising a fatty acid covalently attached to an IL-22 protein by a linker, wherein

(i) the IL-22 is a variant of native mature human IL-22 (hIL-22; SEQ ID NO. 1) wherein the variant comprises 1, 2, or 3 amino acid substitutions including a Cys substitution at position 1 of hIL-22;

(ii) the linker is covalently attached to the substituted Cys residue at position 1 of hIL-22;

(iii) the fatty acid has the structure HOOC—(CH 2 ) x —CO—*, wherein x is 16 and * designates the point of attachment to the linker; and

(iv) the variant comprises a G-P-G N-terminal peptide.

2. A derivative as claimed in claim 1 , wherein the variant comprises 3 amino acid substitutions including a Cys substitution at position 1 of hIL-22, and N35Q and N64Q (where the amino acid position is defined relative to SEQ ID NO. 1).

3. A derivative as claimed in claim 1 , wherein the linker and fatty acid together have a structure given by Formula 1A below, where * designates the point of covalent attachment to the derivative:

4. A derivative as claimed in claim 3 , wherein:

(i) The variant has the sequence set forth in SEQ ID NO. 16, the linker is attached at position 4, and the linker and fatty acid together are according to Formula 1A;

(ii) The variant has the sequence set forth in SEQ ID NO. 18, the linker is attached at position 4, and the linker and fatty acid together are according to Formula 1A.

5. A derivative as claimed in claim 1 , wherein the derivative is:

6. A derivative as claimed in claim 1 , wherein the derivative is:

Assignments (1)

Priority Claims (1)

Continuity (4)

References Cited (57)