IP Library › Granted Patent US 12,233,278
Granted Patent B2
US 12,233,278 · App. 18/413,943 · Granted Feb 25, 2025

Laser system for enhancing remineralization and strength of hard tissue

Inventors: Charles Kerbage (Arlington, MA); Ali Badreddine (Boston, MA); Stephen Couitt (Auburndale, MA); Roni Cantor-Balan (Natick, MA)
Assignee: Convergent Dental, Inc.
A61N5/0603A61C19/063A61N2005/0606A61N5/067
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Quick Facts
Patent No.
US 12,233,278
App. No.
18/413,943
Granted
Feb 25, 2025
Kind
B2
Abstract

A laser-based system and method for treatment of hard tissue enhances remineralization and fluoride uptake to improve resistance to demineralization. The system can include a laser source, an optic, and a controller for controlling the laser source and/or the optic to deliver radiation to a treatment region with desirable characteristics (e.g., to remove carbonate without damaging the tissue surface). In some cases, the system includes a handpiece having an optical element (e.g., a lens) mounted within a replaceable cartridge and adapted to modulate a laser beam to render the beam non-ablative, prior to the laser beam's delivery to the treatment region. In some embodiments, treatment with the laser can be combined with a fluoride treatment for enhanced therapeutic effect.

Claims (24)

1. A method for treating a hard dental tissue, the method comprising the steps of:

generating a laser beam using a laser source;

directing the laser beam to a treatment surface of the hard dental tissue using an optic in optical communication with the laser source; and

controlling the laser source and the optic using a controller to deliver the laser beam to the treatment surface to treat an area of the hard dental tissue at a rate in a range from 10 cm 2 /min to 20 cm 2 /min with a fluence in a range from 0.4 J/cm 2 up to 1.2 J/cm 2 to:

remove at least some carbonate from the treatment surface to generate an acid resistant surface without damaging the hard dental tissue;

thereafter determining a depth mineral loss value of the hard dental tissue; and

confirming the depth mineral loss value of the hard dental tissue is at least 10% relative to untreated hard dental tissue.

2. The method of claim 1 , wherein the laser source comprises a CO 2 laser source.

3. The method of claim 2 , wherein the laser beam comprises a wavelength in a range from 9 μm to 11 μm.

4. The method of claim 1 , wherein the optic comprises at least one of a galvanometer and a turning mirror.

5. The method of claim 1 , wherein the controller is further adapted to control the laser source to deliver the laser beam to the treatment surface in a series of pulses.

6. The method of claim 5 , wherein each pulse in the series of pulses comprises a pulse energy in a range from 0.1 mJ to 50 mJ.

7. The method of claim 5 , wherein each pulse in the series of pulses comprises a pulse duration in a range from 1 μsec to 100 μsec.

8. The method of claim 5 , wherein the series of pulses comprises a repetition rate in a range from 0.05 Hz to 10 Hz.

9. The method of claim 5 , wherein the series of pulses comprises a duty cycle in arrange from 0.1 to 10.

10. The method of claim 5 , wherein the controller is adapted to deliver the series of pulses to the treatment surface in a pattern.

11. The method of claim 10 , wherein the pattern comprises a diameter in a range from 1 mm to 5 mm.

12. The method of claim 10 , wherein the pattern comprises a number of locations in a range from 1 to 1,000.

13. The method of claim 12 , wherein the pattern comprises 217 locations.

14. The method of claim 12 , wherein a spacing between each location in the pattern is in a range from 0.1 mm to 0.5 mm.

15. The method of claim 1 , wherein the controlling step further comprises using the controller to control the laser source to deliver the laser beam to the treatment surface to, when combined with a fluoride treatment, reduce a depth mineral loss value of the dental tissue by at least 20% relative to hard dental tissue subject to the fluoride treatment.

16. The method of claim 15 , further comprising the step of delivering the fluoride treatment to the treatment surface.

17. The method of claim 1 , wherein the controlling step further comprises using the controller to control the laser source to deliver the laser beam to the treatment surface to reduce a surface mineral loss value of the dental tissue by at least 68% relative to untreated dental tissue.

18. The method of claim 1 , wherein the controlling step further comprises using the controller to control the laser source to deliver the laser beam to the treatment surface to, when combined with a fluoride treatment, reduce a surface mineral loss value of the dental tissue by at least 18% relative to hard dental tissue subject to the fluoride treatment.

Assignments (2)
SECURITY INTEREST Recorded Jun 13, 2025
From: CONVERGENT DENTAL, INC.
To: SYMBIOTIC CAPITAL AGENCY LLC, AS COLLATERAL AGENT
Reel/Frame 071413/0530 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 23, 2024
From: KERBAGE, CHARLES; BADREDDINE, ALI; COUITT, STEPHEN; CANTOR-BALAN, RONI
To: CONVERGENT DENTAL, INC.
Reel/Frame 068056/0357 →
Continuity (3)
Continuation 17139208 · Dec 31, 2020
Provisional Application 62956862 · Jan 3, 2020
Related Publication 20240226597A1 · Jul 11, 2024
References Cited (124)
US 4877401A · Higuchi et al. · 1989 [cited by applicant]
US 5374266A · Kataoka et al. · 1994 [cited by applicant]
US 5388987A · Badoz et al. · 1995 [cited by applicant]
US 5435724A · Goodman et al. · 1995 [cited by applicant]
US 6669685B1 · Rizoiu et al. · 2003 [cited by applicant]
US 7931645B2 · Strassl et al. · 2011 [cited by applicant]
US 8011923B2 · Lukac et al. · 2011 [cited by applicant]
US 10045833B2 · Monty et al. · 2018 [cited by applicant]
US 10779908B2 · Dresser et al. · 2020 [cited by applicant]
US 11918824B2 · Kerbage · 2024 [cited by examiner]
US 20020164291A1 · Cozean et al. · 2002 [cited by applicant]
US 20030170586A1 · Cozean et al. · 2003 [cited by applicant]
US 20040248060A1 · Cozean et al. · 2004 [cited by applicant]
US 20050255053A1 · Cozean et al. · 2005 [cited by applicant]
US 20070160958A1 · Belikov et al. · 2007 [cited by applicant]
US 20080280260A1 · Belikov · 2008 [cited by examiner]
US 20110076645A1 · Torres Zaragoza · 2011 [cited by applicant]
US 20110189628A1 · Monty · 2011 [cited by applicant]
US 20110207075A1 · Altshuler et al. · 2011 [cited by applicant]
US 20130059264A1 · Monty · 2013 [cited by applicant]
US 20140093843A1 · Altshuler et al. · 2014 [cited by applicant]
US 20150147718A1 · Khakpour et al. · 2015 [cited by applicant]
US 20160135937A1 · Jeng et al. · 2016 [cited by applicant]
US 20160143703A1 · Convergent · 2016 [cited by applicant]
US 20170319277A1 · Cantor-Balan et al. · 2017 [cited by applicant]
US 20180325622A1 · Groves, Jr. · 2018 [cited by examiner]
US 20190117333A1 · Groves, Jr. et al. · 2019 [cited by applicant]
CA 2867703A1 · 2013 [cited by applicant]
EP 2030591 · 2009 [cited by applicant]
JP 2004049577A · 2004 [cited by applicant]
JP 2009167135A · 2009 [cited by applicant]
JP 2016163749 · 2016 [cited by applicant]
WO 2003059305A1 · 2003 [cited by applicant]
WO 2003076015A1 · 2003 [cited by applicant]
WO 2012008599 · 2012 [cited by applicant]
WO 2012105972A1 · 2012 [cited by applicant]
Correa-Afonso et al, “Influence of Laser Irradiation on Pits and Fissures: An In Situ Study” <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565555/ Feb. 2013. [cited by applicant]
Daniel Nguyen et al., “High-speed scanning ablation of dental hard tissues with a ? = 9.3 ?m CO2 laser: adhesion, mechanical strength, heat accumulation, and peripheral thermal damage”; Journal of Biomedical Optics 16 (… [cited by applicant]
Lidiany Azevedo, et al, “Carbon dioxide laser in dental caries prevention ”, Oct. 2004 <https://www.researchgate.net/publication/8407439 Carbon_dioxide_laser_in_dental_caries_prevention>. [cited by applicant]
Marcella Esteves-Oliveira et al, “Screening of CO2 Laser (10.6??m) Parameters for Prevention of Enamel Erosion” Photomedicine and Laser Surgery, vol. 30, Nov. 6, 2012, pp. 330-338. [cited by applicant]
Kwang K. Chang, et al. “Adhesion studies on dental enamel surfaces irradiated by a rapidly scanned carbon dioxide laser”, Proc SPIE Int Soc Opt Eng. Jan. 1, 2011; 7884: doi:10,1117/12.878892. [cited by applicant]
Kenneth H. Chan, et al., “Analysis of enamel surface damage after selective laser ablation of composite from tooth surfaces”, Photonics Lasers Med. Feb. 1, 2014; 3(1): 37-45. [cited by applicant]
Daniel Fried, et al., “Infrared Spectroscopy Of Laser Irradiated Dental Hard Tissues Using The Advanced Light Source” Apr. 27, 2001 <http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.158.7297&rep=rep1&type=pdf>. [cited by applicant]
Michael Staninec, et al., “Pulpal Effects Of Enamel Ablation With A Microsecond Pulsed ?=9.3-?m CO2 Laser” Lasers Surg Med. Apr. 2009; 41 (4): 256-263. [cited by applicant]
Shlomo Assa, et al. “Ablation Of Dental Hard Tissues With A Microsecond Pulsed Carbon Dioxide Laser Operating At 9.3-?m With An Integrated Scanner” Proc SPIE Int Soc Opt Eng. 2008; 6843:684308. doi: 10.1117/12.778799. [cited by applicant]
Saba Hedayatollahnajafi, et al. “Dentin Bond Strength After Ablation Using A Co2 Laser Operating At High Pulse Repetition Rates” Proc SPIE Int Soc Opt Eng. Feb. 18, 2009; 7162:71620F-.doi:1117/12.816862. [cited by applicant]
Saba Hedayatollahnajafi, et al.“Relation between Acid Dissolution and Histological Alteration of Heated Tooth Enamel (with 1 color plate)” Proc SPIE Int Soc Opt Eng. Feb. 18, 7162: 71620F-.doi:10.11/12.816862. [cited by applicant]
Fowler Bo, et al., “Changes in heated and in laser-irradiated human tooth enamel and their probable effects on solubility” Calcif Tissue Int. Apr. 1986; 38(4):197-208. [cited by applicant]
Giselle rodrigues de Sant'Anna, et al. “Dental Enamel Irradiated with Infrared Diode Laser and Photo-Absorbing Cream: Part 2-EDX Study” Photomed Laser Surg. Oct. 2009; 27(5): 771-782. [cited by applicant]
Kenneth H. Chan, et al., “Rapid and Selective Removal of Composite From Tooth Surfaces With a 9.3 ?m CO2 Laser Using Spectral Feedback” Lasers Surg Med. Sep. 2011; 43(8): 824-832. [cited by applicant]
Kenneth H. Chan, et al. “Selective Removal of Demineralization Using Near Infrared Cross Polarization Reflectance and a Carbon Dioxide Laser”. [cited by applicant]
Leon C. Chung, et al, “Image-guided removal of occlusal caries lesions with a ?= 9.3-μm CO2 laser using near-IR transillumination” Proc SPIE Int Soc Opt. Eng. Feb. 24, 2015; 9306. [cited by applicant]
Saman K. Manesh, et al, “Nondestructive assessment of dentin demineralization using polarization sensitive optical coherence tomography after exposure to fluoride and laser irradiation” J. Biomed Mater Res B. Appl Bioma… [cited by applicant]
Peter Rechmann, et al., :“Caries inhibition in vital teeth using 9.6-?m CO2-laser irradiation” Jourmal of Biomedical Optics 16 (7), 071405 (Jul. 2011). [cited by applicant]
JD Featherstone, et al., “CO2 laser inhibitor of artificial caries-like lesion progression in dental enamel” J Dent Res. Jun. 1998;77(6):1397-403. [cited by applicant]
S.M. McCormack, et. al., “Scanning Electron Microscope Observations Of Co2 Laser Effects On Dental Enamel” J Dent Res 74(10): 1702-1708, Oct. 1995. [cited by applicant]
M. Hossain, et al. “Effect of Pulsed Nd:YAG Laser Irradiation on Acid Demineralization of Enamel and Dentin” J Clin Laser Med Surg. Apr. 2001;19(2):105-8. [cited by applicant]
D. Fried, et al., “Dissolution Studies Of Bovine Dental Enamel Surfaces Modified By High-Speed Scanning Ablation With A Lambda = 9.3-Microm TEA CO(2) Laser” Lasers Surg Med. Oct. 2006;38(9):837-45. [cited by applicant]
S. Tagomori, et al., “Ultrastructural Change of Enamel Exposed to a Normal Pulsed IMd-YAG Laser” Caries Res 1995;29:513-520. [cited by applicant]
Byung J. Nahm, et al. “Investigation of Acid-Etched CO2 Laser Ablated Enamel Surfaces Using Polarization Sensitive Optical Coherence Tomography” Proc SPIE Int Soc Opt Eng. Feb. 9, 2012; 8208. [cited by applicant]
Kenneth H. Chan, et al., “Selective Removal of Dental Composite using a Rapidly Scanned Carbon Dioxide Laser” Proc SPIE Int Soc Opt Eng. 2011 ; 7884. [cited by applicant]
Mozammal Hossain, et al., “Acquired Acid Resistance of Dental Hard Tissues by CO2 Laser Irradiation” Journal of Clinical Laser Medicine & Surgery </journal/pho.1>vol. 17, No. 5 | <https://www.liebertpub.com/toc/pho.1/17… [cited by applicant]
J.H. Meurman, et al., “Transformation of Hydroxyapatite to Fluorapatite by Irradiation with High-Energy CO2 Laser” Caries Res 1997;31:397-400. [cited by applicant]
Daniel Fried, et al., “Multiple-Pulse Irradiation Of Dental Hard Tissues At Co2 Laser Wavelengths” Proc. SPIE 2394, Lasers in Dentistry, (May 1, 1995). [cited by applicant]
Daniel Fried, et al., “Thermal And Chemical Modification Of Dentin Ba Pulsed Co2 Laser Irradiation At 9 To 11 Um” Proc. SPIE 2973, Lasers in Dentistry III, (May 15, 1997). [cited by applicant]
Pinabel Viraparia, et. al., “CO2 Laser: Evidence Based Applications In Dentistry” CO2 Laser—Optimisation and Application, Dr. Dan C. Dumitras (Ed.), ISBN: 978-953-51-0351-6, InTech, Available from: http://www.intechopen… [cited by applicant]
Linn H. Maung, et al. “Near-Ir Imaging Of Thermal Changes In Enamel During Laser Ablation” Proc SPIE Int Soc Opt Eng. Mar. 5, 2010; 7546(1). [cited by applicant]
JD Featherstone, “Lasers In Dentistry 3. The Use Of Lasers For The Prevention Of Dental Caries” Ned Tijdschr Tandheelkd. May 2002;109(5):162-7. [cited by applicant]
JD Featherstone, et al., “Rational Choice Of Laser Conditions For Inhibition Of Caries Progression” Proc. SPIE 2394, Lasers in Dentistry, (May 1, 1995). [cited by applicant]
Joyce Y. Cheng, et al., “Use Of A Compact Fiber Optic Spectrometer For Spectral Feedback During The Laser Ablation Of Dental Hard Tissues And Restorative Materials” . SPIE 6137, Lasers in Dentistry XII, 61370F (Feb. 13,… [cited by applicant]
JD Featherstone, et al. “Mechanism Of Laser-Induced Solubility Reduction Of Dental Enamel” Proc. SPIE 2973, Lasers in Dentistry III, (May 15, 1997). [cited by applicant]
Michael J. Zuerlein, et al. “Modeling thermal emission in dental enamel induced by 9-11 ?m laser light” Applied Surface Science 127:863-868 ⋅ May 1998. [cited by applicant]
JD Featherstone, et al., “Effect Of Pulse Duration And Repetition Rate On Co2 Laser Inhibition Of Caries Progression” Proc. SPIE 2672, Lasers in Dentistry II, (Apr. 23, 1996). [cited by applicant]
JD Featherstone, et al., “Surface Dissolution Kinetics Of Dental Hard Tissue Irradiated Over A Fluence Range Of 1 To 8 J/Cm2” Proc. SPIE 3248, Lasers in Dentistry IV, (Apr. 22, 1998). [cited by applicant]
Daniel Fried, et al., “Thermal response of hard dental tissues to 9? through 11??m CO2?laser irradiation” Optical Engineering 35(7), (Jul. 1, 1996). <https://doi.org/10.1117/1.600774>. [cited by applicant]
International Search Reporting and Written Opinion issued for PCT/US2018/032022, mailed Sep. 18, 2018. [cited by applicant]
Ertl, et al., “Hard Tissue Ablation With Pulsed CO2 Lasers”, SPIE vol. 1800 pp. 176-181 (. [cited by applicant]
Gerold K.H. Eyrich, “Laser-osteotomy induced changes in bone”, Medical Laser Application 20 (2005) 25-36. [cited by applicant]
M. Frentzen, et al., “Osteotomy with 80ms CO2 laser pulses—histological results”, Lasers Med Sci (2003)18:119-124. [cited by applicant]
Werner, et al., “CO2 laser free-form processing of hard tissue”, Therapeutic Laser Applications and Laser-Tissue Interactions III, Feb. 24, 2010 vol. 6632 663202-1-663202-6. [cited by applicant]
Ivanenko, et al., Ablation of hard bone tissue with puled CO2 Lasers, Medical Laser Application 20 (2005) 13-23. [cited by applicant]
G. D. Rajitha Gunaratne, Riaz Khan, Daniel Fick, Brett Robertson, Narendra Dahotre & Charlie Ironside (2016): A review of the physiological and histological effects of laser osteotomy, Journal of Medical Engineering & T… [cited by applicant]
Ivanenko, et al., “Hard tissue ablation with sub-ms CO2 laser pulses with the use of air-water spray”, Optical Biopsy and Tissue Optics, Proceedings of SPIE vol. 4161 (2000). [cited by applicant]
Ivanenko, et al., “In Vivo animal trials with a scanning CO2 laser Osteotome,” Lasers in Surgery and Medicine 37:144-148 (2005). [cited by applicant]
Ivanenko, et al., “System development and clinical studies with a scanning CO2 laser osteotome,” Optical Interactions with Tissue and Cells XVII, Proc. of SPIE vol. 6084, 60840H, (2006) 1605-7422. [cited by applicant]
Kahrs, et al., “Planning and simulation of microsugrical laser bone ablation,” Int J CARS (2010) 5:155-162 (DOI 10.1007/s11548-009-0303-4). [cited by applicant]
Kuttenberger, et al., “Bone healing of the sheep tibia shaft after carbon dioxide laser osteotomy; histological results,” Lasers Med Sci (2010) 25:239-249 (DOI 10.1007/s10103-009-0714-z). [cited by applicant]
Nair, et al., “Observations on pulpal response to carbon dioxide laser drilling of dentine in healthy human third molars,” Lasers in Medical Science (2005) 19: 240-247 (DOI 10.1007/s10103-004-0317-7). [cited by applicant]
Werner, et al., “CO2 laser “milling” of hard tissue” Optical Interactions with Tissue and Cells XVIII, Proc. of SPIE vol. 6435, 64350E, (2007) 1605-7422. [cited by applicant]
Zhang, et al., “Optimization of Line Cut Strategy for Bone tissue ablation using Short-pulsed CO2 laser based on thermal relaxation,”. [cited by applicant]
Kuttenberger, et al., “Computer-Guided CO2-laser osteotomy of the SheepTibia: Technical prerequisites and first resultes,” Photomedicine and Laser Surgery, vol. 26, No. 2, 2008, pp. 129-136 (DOI: 10.1089/pho.2007.2139). [cited by applicant]
Zhu Y, Zhang X, Chen Y, et al. A comparative study on the dissolution and solubility of hydroxylapatite and fluorapatite at 25° C. and 45° C. Chem Geol. 2009;268(1-2):89-96. doi:10.1016/j.chemgeo.2009.07.014. [cited by applicant]
Shellis RP, Barbour ME, Jones SB, Addy M. Effects of pH and acid concentration on erosive dissolution of enamel, dentine, and compressed hydroxyapatite. Eur J Oral Sci. 2010; 118(5):475-482. doi:10.1111/j.1600-0722.2010… [cited by applicant]
Shellis RP r., Featherstone JDB, Lussi A. Understanding the Chemistry of Dental Erosion. Erosive Tooth Wear From Diagnosis to Ther. 2012;25:163-179. doi:10.1159/000359943. [cited by applicant]
Shi J, Klocke A, Zhang M, Bismayer U. Thermally-induced structural modification of dental enamel apatite: Decomposition and transformation of carbonate groups. Eur J Mineral. 2005; 17(5):769-776. doi:10.1127/0935-1221/2… [cited by applicant]
Barinov SM, Rau J V., Cesaro SN, et al. Carbonate release from carbonated hydroxyapatite in the wide temperature rage. J Mater Sci Mater Med. 2006; 17(7):597-604. doi:10.1007/s10856-006-9221-y. [cited by applicant]
Featherstone JDB, Fried D. Fundamental Interactions of Lasers with Dental Hard Tissues. Med Laser Appl. 2001; 16(3):181-194. doi:http://dx.doi.org/10.1078/1615-1615-00022. [cited by applicant]
Kayano T, Ochiai S, Kiyono K, Yamamoto H, Nakajima S, Mochizuki T. [Effects of Er:YAG laser irradiation on human extracted teeth]. Kokubyo Gakkai Zasshi. 1989;56(2):381-392. http://www.ncbi.nlm.nih.gov/pubmed/2794701. A… [cited by applicant]
Rechmann P, Fried D, Le CQ, et al. Caries inhibition in vital teeth using 9.6-?m CO2-laser irradiation. J Biomed Opt. 2011;16(7):071405. doi: 10.1117/1.3564908. [cited by applicant]
Rechmann P, Charland DA, Rechmann BMT, Le CQ, Featherstone JDB. In-vivo occlusal caries prevention by pulsed CO 2 -laser and fluoride varnish treatment—A clinical pilot study. Lasers Surg Med. 2013;45(5):302-310. doi:10… [cited by applicant]
González-Rodríguez A, de Dios López-González J, del Castillo J de DL, Villalba-Moreno J. Comparison of effects of diode laser and CO2 laser on human teeth and their usefulness in topical fluoridation. Lasers Med Sci. 20… [cited by applicant]
Cohen J, Featherstone JDB, Le CQ, Steinberg D, Feuerstein O. Effects of CO 2 laser irradiation on tooth enamel coated with biofilm. Lasers Surg Med. 2014;46(3):216-223. doi:10.1002/lsm.22218. [cited by applicant]
Stern RH, Vahl J, Sognnaes RF. Lased Enamel: Ultrastructural Observations of Pulsed Carbon Dioxide Laser Effects. J Dent Res. 1972;51(2):455-460. doi:10.1177/00220345720510023501. [cited by applicant]
Beeking PO, Herrmann C, Zuhrt R. [Examination of laser-treated tooth surfaces after exposure to acid]. Dtsch Stomatol. 1990;40(12):490-492. http://www.ncbi.nlm.nih.gov/pubmed/2132112. Accessed Aug. 28, 2019. [cited by applicant]
Steiner-Oliveira C, Nobre-dos-Santos M, Zero DT, Eckert G, Hara AT. Effect of a pulsed CO2 laser and fluoride on the prevention of enamel and dentine erosion. Arch Oral Biol. 2010;55(2):127-133. doi: 10.1016/j.archoralb… [cited by applicant]
Ana PA, Bachmann L, Zezell DM. Lasers effects on enamel for caries prevention. Laser Phys. 2006;16(5):865-875. doi:10.1134/S1054660X06050197. [cited by applicant]
Hossain M, Nakamura Y, Kimura Y, Yamada Y, Ito M, Matsumoto K. Caries-Preventive Effect of Er:YAG Laser Irradiation with or without Water Mist. J Clin Laser Med Surg. 2000;18(2):61-65. doi:10.1089/clm.2000.18.61. [cited by applicant]
Corrêa-Afonso AM, Bachmann L, De Almeida CG, Corona SAM, Borsatto Mc. FTIR and SEM analysis of CO2 laser irradiated human enamel. Arch Oral Biol. 2012;57(9):1153-1158. doi:10.1016/j.archoralbio.2012.02.004. [cited by applicant]
Zezell DM, Benetti C, Veloso MN, Castro PAA, Ana PA. FTIR spectroscopy revealing the effects of laser and ionizing radiation on biological hard tissues. J Braz Chem Soc. 2015;26(12):2571-2582. doi:10.5935/0103-5053.2015… [cited by applicant]
Nelson DGA, Wefel JS, Jongebloed WL, Featherstone JDB. Morphology, Histology and Crystallography of Human Dental Enamel Treated with Pulsed Low-Energy Infrared Laser Radiation. Caries Res. 1987;21(5):411-426. doi:10.115… [cited by applicant]
Featherstone JDB. The science and practice of caries prevention. J Am Dent Assoc. 2000; 131(7):887-899. doi:10.14219/jada.archive.2000.0307. [cited by applicant]
Featherstone JDB, Barrett-Vespone NA, Fried D, Kantorowitz Z, Seka W. CO2 laser inhibition of artificial caries-like lesion progression in dental enamel. J Dent Res. 1998;77(6):1397-1403. doi:10.1177/0022034598077006040… [cited by applicant]
Esteves-Oliveira M, Pasaporti C, Heussen N, Eduardo CP, Lampert F, Apel C. Rehardening of acid-softened enamel and prevention of enamel softening through CO2 laser irradiation. J Dent. 2011;39(6):414-421. doi:10.1016/j.… [cited by applicant]
Rechmann P, Rechmann BMT, Groves WH, et al. Caries inhibition with a CO 2 9.3??m laser: An in vitro study. Lasers Surg Med. 2016;554(February):1-9. doi:10.1002/lsm.22497. [cited by applicant]
Zuerlein M, Fried D, Featherstone JDB. Modeling the Modification Depth of carbon Dioxide Laser Treated Enamel. Lasers Surg Med. 1999;25(May):335-347. [cited by applicant]
Argenta RMO, Tabchoury CPM, Cury JA. A modified pH-cycling model to evaluate fluoride effect on enamel demineralization. Pesqui Odontol Bras. 2003;17(3):241-246. doi:10.1590/S1517-74912003000300008. [cited by applicant]
Stookey GK, Featherstone JDB, Rapozo-Hilo M, et al. The Featherstone laboratory pH cycling model: A prospective, multi-site validation exercise. Am J Dent. 2011;24(5):322-328. [cited by applicant]
Featherstone JD, ten Cate JM, Shariati M, Arends J. Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res. 1983;17(5):385-391. doi:10.1159/000260692. [cited by applicant]
Bahrololoomi Z, Fotuhi Ardakani F, Sorouri M, Fotuhi Ardakani F. In Vitro Comparison of the Effects of Diode Laser and CO 2 Laser on Topical Fluoride Uptake in Primary Teeth. J Dent. 2015;12(8). www.jdt.tums.ac.ir. Acce… [cited by applicant]
Tepper SA, Zehnder M, Pajarola GF, Schmidlin PR. Increased fluoride uptake and acid resistance by CO 2 laser-irradiation through topically applied fluoride on human enamel in vitro. J Dent. 2004;32(8):635-641. doi:10.10… [cited by applicant]
International Search Report and Written Opinion for PCT/US2020/067656 dated Apr. 21, 2021. [cited by applicant]
Rechmann, P., et al., “In vitro CO2 9.3-mm short-pulsed laser caries prevention—effects of a newly developed laser irradiation pattern”, Lasers in Medical Science (2020) 35, 13 pages. [cited by applicant]
L.K.A. Rodrigues, et al., “In Situ Mineral Loss Inhibition by CO2 Laser and Fluoride”, 2006, J Dent Res, 85(7): 617-621. [cited by applicant]
Rodrigues, L.K.A., et al., “In situ Mineral Loss Inhibition by CO2 Laser and Fluoride”, Journal of Dental Research 85(7):617-621, 2006. [cited by applicant]