Recombinant microorganisms and uses therefor
The disclosure provides genetically engineered C1-fixing microorganisms capable of producing nanobodies. Additionally, the disclosure provides engineered microorganisms comprising one or more disrupted genes to strategically divert carbon flux away from nonessential or undesirable products towards products and/or co-products of interest. The disclosure enables co-production of useful chemicals from gaseous substrates.
1. A genetically engineered C1-fixing microorganism capable of co-producing at least one target product and a nanobody comprising an exogenous nucleic acid encoding a nanobody gene, wherein the genetically engineered C1-fixing microorganism has improved carbon flux through acetoacetyl-CoA compared to a parental microorganism, wherein the nanobody gene is VHH-72 SARS Cov2.
2. The genetically engineered C1-fixing microorganism of claim 1 , wherein the at least one target product is selected from the group 1-butanol, butyrate, butene, butadiene, methyl ethyl ketone, ethylene, acetone, isopropanol, lipids, 3-hydroxypropionate, terpenes, isoprene, fatty acids, 2-butanol, 1,2-propanediol, 1-propanol, 1-hexanol, 1-octanol, chorismate-derived products, 3-hydroxybutyrate, 1,3-butanediol, 2-hydroxyisobutyrate or 2-hydroxyisobutyric acid, isobutylene, adipic acid, keto-adipic acid, 1,3-hexanediol, 3-methyl-2-butanol, 2-buten-1-ol, isovalerate, isoamyl alcohol, and monoethylene glycol.
3. The genetically engineered C1-fixing microorganism of claim 1 , wherein the nanobody is contained in the microorganism.
4. The genetically engineered C1-fixing microorganism of claim 1 , further comprising a disruptive mutation in one or more genes.
5. The genetically engineered C1-fixing microorganism of claim 1 , wherein the parental microorganism is selected from the group consisting of Acetobacterium woodii, Alkalibaculum bacchii, Blautia producta, Butyribacterium methylotrophicum, Clostridium aceticum, Clostridium autoethanogemum, Clostridium carboxidivorans, Clostridium coskatii, Clostridium drakei, Clostridium formicoaceticum, Clostridium ljungdahlii, Clostridium magmnum, Clostridium ragsdalei, Clostridium scatologenes, Eubacterium limosum, Moorella thermautotrophica, Moorella thermoacetica, Oxobacter pfennigii, Sporomusa ovata, Sporomusa silvacetica, Sporomusa sphaeroides, Cupriavidus necator and Thermoanaerobacter kivui.
6. The genetically engineered C1-fixing microorganism of claim 1 , wherein the parental microorganism is selected from the group consisting of Clostridium autoethanogemum, Clostridium ljungdahlii , and Clostridium ragsdalei.
7. A method of co-producing at least one target product and a nanobody by culturing the genetically engineered C1-fixing microorganism of claim 1 in the presence of a gaseous substrate comprising one or more of CO, CO 2 , and H 2 .
8. The method of claim 7 , wherein the at least one target product is selected from the group 1-butanol, butyrate, butene, butadiene, methyl ethyl ketone, ethylene, acetone, isopropanol, lipids, 3-hydroxypropionate, terpenes, isoprene, fatty acids, 2-butanol, 1,2-propanediol, 1-propanol, 1-hexanol, 1-octanol, chorismate-derived products, 3-hydroxybutyrate, 1,3-butanediol, 2-hydroxyisobutyrate or 2-hydroxyisobutyric acid, isobutylene, adipic acid, keto-adipic acid, 1,3-hexanediol, 3-methyl-2-butanol, 2-buten-1-ol, isovalerate, isoamyl alcohol, or monoethylene glycol.