IP Library Granted Patent US 12,245,567
Granted Patent B2
US 12,245,567 · App. 17/587,964 · Granted Mar 11, 2025

Alfalfa variety R414W279

Inventors: Julie Ho (Davis, CA); Peter Reisen (Nampa, ID)
Assignee: Forage Genetics International, LLC
A01H6/544A01H5/10
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,245,567
App. No.
17/587,964
Granted
Mar 11, 2025
Kind
B2
Abstract

The invention relates to the alfalfa variety designated R414W279. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R414W279. Also provided by the invention are tissue cultures of the alfalfa variety R414W279 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R414W279 with itself or another alfalfa variety and plants produced by such methods.

Claims (30)

1. A population of seeds of alfalfa variety R414W279, wherein representative seed of said alfalfa variety have been deposited under NCMA Accession No. 202110010.

2. A population of plants grown from the population of seeds of claim 1 .

3. A composition comprising the population of seeds of claim 1 that is comprised in plant seed growth media.

4. The composition of claim 3 , wherein the plant seed growth media is soil or a synthetic cultivation media.

5. A method for producing a population of first generation progeny alfalfa seeds, the method comprising crossing the population of plants of claim 2 with a second alfalfa plant population and harvesting the resultant alfalfa seed.

6. The method of claim 5 , wherein the second alfalfa plant population is a population of plants of alfalfa variety R414W279; wherein representative seed of said alfalfa variety have been deposited under NCMA Accession No. 202110010.

7. A population of first generation progeny alfalfa seeds produced by the method of claim 5 ; wherein the method comprises inter-pollinating the population of plants of claim 2 ; and wherein the population of plants grown from the first generation progeny alfalfa seeds have all of the physiological and morphological characteristics of said alfalfa variety R414W279.

8. A population of alfalfa plants produced by growing the seed of claim 7 ; wherein the population of plants produced has all of the physiological and morphological characteristics of said alfalfa variety R414W279.

9. A method of vegetatively propagating the population of plants of claim 2 , the method comprising the steps of:

(a) collecting tissue samples capable of being propagated from the population of plants of claim 2 ;

(b) cultivating the tissue to obtain proliferated shoots; and

(c) rooting the proliferated shoots to obtain rooted plantlets.

10. The method of claim 9 , further comprising growing a plant from the rooted plantlets.

11. A method of modifying a population of alfalfa plants, wherein the method comprises introducing a transgene or a single locus conversion into the population of plants of claim 2 .

12. A population of alfalfa plants produced by the method of claim 11 ; wherein the population of alfalfa plants produced comprises the transgene or single locus conversion and otherwise comprises all of the physiological and morphological characteristics of said alfalfa variety R414W279.

13. The population of plants of claim 12 , wherein the transgene or single locus comprises a nucleic acid sequence that enables site-specific genetic recombination or confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, improved digestibility, improved energy content, improved forage yield, improved seed yield, improved winterhardiness, improved nitrogen fixation, modified fatty acid metabolism, abiotic stress resistance, flowering time, altered seed amino acid composition, and modified carbohydrate metabolism.

14. A population of seeds that produces the population of plants of claim 12 ; wherein the population of plants produced from the seeds comprises the transgene or single locus conversion and otherwise comprises all of the physiological and morphological characteristics of said alfalfa variety R414W279.

15. A method of introducing a single-locus conversion into the population of plants of claim 2 , the method comprising:

(a) crossing said population of plants of claim 2 with a second alfalfa plant to produce a first generation of progeny plants, wherein the second alfalfa plant comprises the single locus; and

(b) selecting a population of progeny plants that comprises the single locus.

16. The method of claim 15 , wherein the single locus comprises a transgene.

17. A population of alfalfa plants produced by the method of claim 15 ; wherein the population of alfalfa plants produced comprises the single locus conversion and otherwise comprises all of the physiological and morphological characteristics of said alfalfa variety R414W279.

18. A population of seeds that produces the population of plants of claim 17 ; wherein the population of plants produced from the seeds comprises the single locus conversion and otherwise comprises all of the physiological and morphological characteristics of said alfalfa variety R414W279.

19. A method for introducing a transgene or a single locus conversion into a population of alfalfa plants, the method comprising the steps of:

(a) modifying a plant of the population of plants of claim 2 by introducing a transgene or a single locus conversion; and

(b) crossing the modified population of alfalfa plants of step (a) with a population of alfalfa plants to produce a population of progeny plants, wherein at least a progeny plant comprises the transgene or single locus conversion.

20. The method of claim 19 , further comprising the step of:

(c) applying a selection technique to the population produced in step (b) to select said progeny plants that comprise the transgene or single locus conversion.

21. A method of producing a synthetic alfalfa variety, the method comprising intercrossing the seeds of claim 1 with seeds of a second alfalfa variety.

22. A method of producing a commodity plant product, the method comprising obtaining the population of plants of claim 2 and producing a commodity plant product from the population of plants, wherein the commodity plant product is selected from the group consisting of sprouts, forage, hay, greenchop, and silage.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 2, 2023
From: HO, JULIE; REISEN, PETER
To: FORAGE GENETICS INTERNATIONAL LLC
Reel/Frame 063513/0725 →
Continuity (1)
Related Publication 20230240247A1 · Aug 3, 2023
References Cited (22)
US 7566817B2 · Beazley et al. · 2009 [cited by applicant]
US 8124848B2 · Beazley et al. · 2012 [cited by applicant]
US 9068196B2 · Beazley et al. · 2015 [cited by applicant]
US 20200229389A1 · Rodgers · 2020 [cited by examiner]
Chukwu, Samuel Chibuike, et al. “Recovery of recurrent parent genome in a marker-assisted backcrossing against rice blast and blight infections using functional markers and SSRs.” Plants 9.11 (2020): 1411. (Year: 2020). [cited by examiner]
Kuznetsov, Igor, et al. “Identification of promising alfalfa varieties in conditions of the southern forest-steppe zone (Republic of Bashkortostan): a study of economic and biological characteristics.” Botanical studies… [cited by examiner]
Plant and Soil Sciences eLibrary: Advanced backcross breeding lesson (2023); pp. 1-3; downloaded on Dec. 6, 2023 (Year: 2023). [cited by examiner]
Wang, Xiaojuan, et al. “Genetic diversity among alfalfa ( [cited by examiner]
U.S. Appl. No. 17/587,985, filed Jan. 28, 2022, Rodgers et al. [cited by applicant]
U.S. Appl. No. 17/587,939, filed Jan. 28, 2022, Rodgers et al. [cited by applicant]
U.S. Appl. No. 17/587,944, filed Jan. 28, 2022, Rodgers et al. [cited by applicant]
U.S. Appl. No. 17/587,947, filed Jan. 28, 2022, McCaslin et al. [cited by applicant]
U.S. Appl. No. 17/587,955, filed Jan. 28, 2022, McCaslin et al. [cited by applicant]
U.S. Appl. No. 17/587,960, filed Jan. 28, 2022, Ho et al. [cited by applicant]
U.S. Appl. No. 17/587,970, filed Jan. 28, 2022, Rodgers et al. [cited by applicant]
U.S. Appl. No. 17/587,978, filed Jan. 28, 2022, Rodgers et al. [cited by applicant]
U.S. Appl. No. 17/587,981, filed Jan. 28, 2022, Rodgers et al. [cited by applicant]
U.S. Appl. No. 17/587,988, filed Jan. 28, 2022, Ho et al. [cited by applicant]
Association of Official Seed Certifying Agencies, “2021 Alfalfa and Miscellaneous Legumes Variety Review Board Report,” pp. 1-93, available at: https://www.aosca.org/wp-content/uploads/2021/05/2021AL_Report_FINAL.pdf, p… [cited by applicant]
Larkin et al., “Somaclonal variation—a novel source of variability from cell cultures for plant improvement,” [cited by applicant]
Moose et al., “Molecular plant breeding as the foundation for 21st century crop improvement,” [cited by applicant]
Variety specific information as indicated in transmittal letter of Information Disclosure Statement for U.S. Appl. No. 17/587,964, filed Jul. 27, 2022. [cited by applicant]