Methods of modulating seed and organ size in plants
This invention relates to the expression a DA1 protein with a mutation that disrupts or inactivates the LIM domain or the LIM-like domain within cells of a plant. This may increase the yield or enhance a yield-related trait of the plant. Methods, plants and plant cells are provide.
1. A plant, or a plant cell or a plant part thereof, with increased organ size and/or seed size, the plant or plant cell or plant part thereof comprising:
a nucleic acid sequence encoding a LIM-disrupted DA1 protein comprising an amino acid sequence having at least 90% sequence identity to one or more of SEQ ID NOs: 4 to 27 and having a disrupted or inactivated LIM domain, wherein the amino acid sequence of the LIM-disrupted DA1 protein comprises one or more sequence alterations relative to the wild-type LIM domain of SEQ ID NO: 1 that disrupt or inactivate the LIM domain, wherein the wild-type LIM domain comprises two Zn finger motifs, wherein the one or more sequence alterations abolish one or both Zn finger motifs, and wherein the plant has increased organ size and/or seed size compared to a plant without the one or more sequence alterations.
2. The plant of claim 1 , wherein the sequence alterations include a mutation of one or more Zn coordinating residues in the LIM domain.
3. The plant of claim 1 , wherein the sequence alterations include a mutation of one or more non-Zn coordinating residues in the LIM domain.
4. The plant of claim 1 , wherein the LIM-disrupted DA1 protein comprises a C terminal region having the amino acid sequence of residues 198 to 504 of SEQ ID NO: 4, residues 180 to 487 of SEQ ID NO: 5, residues 212 to 514 of SEQ ID NO: 6, residues 229 to 532 of SEQ ID NO: 7, residues 229 to 532 of SEQ ID NO: 8, residues 174 to 478 of SEQ ID NO: 9, residues 174 to 474 of SEQ ID NO: 10, residues 178 to 478 of SEQ ID NO: 11, residues 176 to 462 of SEQ ID NO: 12, residues 179 to 482 of SEQ ID NO: 13, residues 182 to 486 of SEQ ID NO: 14, residues 573 to 878 of SEQ ID NO: 15, residues 181 to 486 of SEQ ID NO: 16, residues 207 to 512 of SEQ ID NO: 17, residues 189 to 491 of SEQ ID NO: 18, residues 181 to 486 of SEQ ID NO: 19, residues 204 to 508 of SEQ ID NO: 20, residues 247 to 553 of SEQ ID NO: 21, residues 219 to 528 of SEQ ID NO: 22, residues 1296 to 1613 of SEQ ID NO: 23, residues 128 to 450 of SEQ ID NO: 24, residues 404 to 702 of SEQ ID NO: 25, residues 343 to 644 of SEQ ID NO: 26, or residues 256 to 587 of SEQ ID NO: 27.
5. The plant of claim 4 , wherein the C terminal region comprises a metallopeptidase motif of SEQ ID NO: 32.
6. The plant of claim 4 , wherein the C terminal region comprises the amino acid sequence of SEQ ID NO: 33 or SEQ ID NO: 34.
7. The plant of claim 1 , wherein the LIM-disrupted DA1 protein comprises a ubiquitin interaction motif 1 (UIM1) domain of SEQ ID NO: 35 and a ubiquitin interaction motif 2 (UIM2) domain of SEQ ID NO: 36.
8. The plant of claim 1 , wherein the nucleic acid encoding the LIM-disrupted DA1 protein is operably linked to a heterologous promoter.
9. The plant of claim 8 , wherein the promoter is a tissue-specific promoter or an inducible promoter.
10. The plant of claim 1 , wherein the plant further comprises reduced or abolished expression or activity of an ENHANCER OF DA1-1 (EOD1) protein, wherein the wild-type EOD1 protein comprises an amino acid sequence having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 38 to 53.
11. The plant of claim 1 , wherein the plant is a monocotyledonous or dicotyledonous plant.
12. The plant of claim 1 , wherein the plant is an agricultural plant selected from Lithospermum erythrorhizon, Taxus spp, tobacco, cucurbits, carrot, vegetable brassica, melons, capsicums, grape vines, lettuce, strawberry, oilseed brassica, sugar beet, wheat, barley, maize, rice, soybean, pea, sorghum, sunflower, tomato, potato, pepper, chrysanthemum, carnation, linseed, hemp, and rye.