Insecticidal proteins
Compositions and methods for controlling insect pests are disclosed. In particular, novel insect inhibitory proteins comprising two different components, both of which are required for biological activity against at least coleopteran insect pests are provided. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. Methods of making the insecticidal proteins and methods of using the insecticidal proteins and nucleic acids encoding the insecticidal proteins of the invention, for example in transgenic plants to confer protection from insect damage, are also disclosed.
1. A recombinant vector comprising:
a first chimeric gene comprising a first heterologous promoter operably linked to a first nucleic acid molecule comprising a nucleotide sequence that (a) encodes a first protein comprising SEQ ID NO:3; or (b) comprises SEQ ID NO:9; and
a second chimeric gene comprising a second heterologous promoter operably linked to a second nucleic acid molecule comprising a nucleotide sequence that (a) encodes a second protein comprising SEQ ID NO:4; or (b) comprises SEQ ID NO: 10.
2. A host cell comprising the recombinant vector of claim 1 , wherein the host cell is a bacterial cell or plant cell.
3. A transgenic plant or plant part comprising the transgenic plant cell of claim 2 .
4. The transgenic plant or plant part of claim 3 that is a transgenic maize plant or plant part.
5. A transgenic plant that produces an insecticidal composition comprising a first component and a second component that function together as an insecticidal toxin, wherein the first component is a protein comprising the sequence of SEQ ID NO:3, and the second component is a protein comprising the sequence of SEQ ID NO:4, and wherein said insecticidal toxin is active against at least a Diabrotica pest insect.
6. The transgenic plant of claim 5 , wherein said plant is a maize plant.
7. A method of producing a transgenic plant or plant part having enhanced insect resistance compared to a control plant or plant part, comprising: (a) introducing into a plant or plant part the recombinant vector of claim 1 , wherein the first protein and second protein are expressed in the plant or plant part, thereby producing a plant or plant part with enhanced insect-resistance.
8. The method of claim 7 , wherein the introducing step is achieved by (a) transforming the plant or plant part; or (b) crossing a first plant comprising the chimeric gene with a different second plant.
9. A method of controlling a Diabrotica insect pest comprising, delivering to the insect pest or an environment thereof an effective insect-controlling amount of an insecticidal composition comprising a first component and a second component that function together as an insecticidal toxin, wherein the first component is a protein comprising the sequence of SEQ ID NO:3, and the second component is a protein comprising the sequence of SEQ ID NO:4, and wherein said insecticidal toxin is active against at least a Diabrotica pest insect.
10. The method of claim 9 , wherein the insecticidal composition is delivered through a transgenic plant or by topical application of a composition comprising the insecticidal composition.