Gene editing components, systems, and methods of use
The present disclosure provides methods and compositions comprising Cas TypeV programmable nucleases and lipid nanoparticles capable of delivering the Cas TypeV programmable nucleases and genome editing systems comprising same.
1. An isolated or recombinant polynucleotide comprising:
a nucleic acid sequence that encodes a polypeptide at least 95% identical to SEQ ID NO: 585, wherein the polypeptide comprises arginine (R) at position 169.
2. The isolated or recombinant nucleic acid sequence of claim 1 , wherein the nucleic acid sequence encodes a polypeptide which comprises:
a. a Wedge (WED), α-helical recognition lobe (REC), PAM-interacting (PI), RuvC nuclease, Bridge Helix (BH) and NUC domains; or
b. one or more domains selected from RuvC, REC, WED, BH, PI and NUC domains.
3. The isolated or recombinant nucleic acid sequence of claim 1 , wherein the nucleic acid sequence is operably fused to a nucleic acid sequence encoding one or more reverse transcriptases.
4. The isolated or recombinant nucleic acid sequence of claim 3 , wherein the one or more reverse transcriptases comprises Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.
5. The isolated or recombinant nucleic acid sequence of claim 1 , wherein the nucleic acid sequence is operably linked to a nucleic acid sequence encoding one or more nuclear localization signals.
6. The isolated or recombinant nucleic acid sequence of claim 1 , wherein the nucleic acid sequence is operably linked to one or more expression control sequences.
7. A vector comprising the isolated or recombinant nucleic acid sequence of claim 3 .
8. The vector of claim 7 , wherein the vector comprises a viral vector, liposomes, lipid nanoparticles (LNPs), cationic polymers, vesicles, or gold nanoparticles.
9. An isolated host cell comprising the isolated or recombinant nucleic acid sequence of claim 3 .
10. The isolated host cell of claim 9 , wherein the host cell comprises a prokaryotic cell, a mammalian cell, or a synthetic cell.
11. The vector of claim 8 , wherein the vector comprises an LNP comprising:
a) one or more ionizable lipids;
b) one or more structural lipids;
c) one or more PEGylated lipids; and
d) one or more phospholipids.
12. A composition comprising the isolated or recombinant nucleic acid sequence of claim 1 ; and a pharmaceutically or veterinarily acceptable carrier.
13. A composition comprising the vector of claim 11 ; and a pharmaceutically or veterinarily acceptable carrier.
14. A polypeptide or an isolated polypeptide comprising:
an amino acid sequence at least 95% identical to SEQ ID NO: 585 and comprising arginine (R) at position 169.
15. The polypeptide or isolated polypeptide of claim 1 , wherein the polypeptide or isolated polypeptide comprises the amino acid sequence of SEQ ID NO:585.
16. The polypeptide or isolated polypeptide of claim 14 , wherein the polypeptide or isolated polypeptide comprises the amino acid sequence of SEQ ID NO:585.