Transparent water nanolipid fluid (DNLF) dispersions and method of preparation using a twin screw extruder
The present invention provides a method of preparing a lipid nanoparticle dispersion using a twin screw extruder. The inventive DNLF fluids are isotropic fluids which include greater than 25% lipophilic content in the form of lipidic nanoparticles dispersed in a continuous aqueous matrix with turbidity less than 375 nephelometric turbidity units (NTU).
1. A lipid nanoparticle dispersion comprising:
from 11.1 to 19.8 wt. % of one or more polyethoxylated high hydrophile-lipophile-balance (HLB) surfactants selected from the group consisting of laureth-23, ceteareth-20, ceteareth-30, steareth-40, PEG32 stearate, PEG75 stearate, PEG100 stearate, PEG15 hydroxy stearate, PEG40 hydrogenated castor oil, polysorbate 20, polysorbate 80, and combinations thereof;
from 3.3 to about 10.1 wt. % of one or more low hydrophile-lipophile-balance (HLB) surfactants selected from the group consisting of cetyl alcohol, glyceryl oleate, glyceryl stearate, glyceryl monolinoleate, oleic acid, stearic acid, sorbitan oleate, soy lecithin, and combinations thereof;
one or more water immiscible oils selected from the group consisting of medium chain triglyceride oil, coconut oil, isopropyl palmitate, isopropyl myristate, methyl decanoate, ethyl myristate, ethyl oleate, mineral oil, orange essential oil, cyclopentasiloxane, poly(dimethyl siloxane), hexadecane, propylene glycol dicaprylate, isododecane, isoeicosane, isohexadecane, soy biodiesel, jojoba oil, cocoyl caprylocaprate, C 10 -C 13 alkane, squalane, sunflower seed oil, and combinations thereof; and
water,
wherein the lipid nanoparticle dispersion has a lipophilic concentration greater than about 25%, a volume average particle size less than about 100 nm, and a turbidity less than 375 nephelometric turbidity units (NTU) at room temperature.
2. The lipid nanoparticle dispersion of claim 1 , further comprising one or more hydrophobic drugs selected from the group consisting of aspirin, benzocaine, ibuprofen, lidocaine, and combinations thereof.
3. The lipid nanoparticle dispersion of claim 1 further comprising one or more hydrophobic therapeutic agents selected from the group consisting of ascorbyl palmitate, birch bark extract, cannabidiol, ubiquinone, cholecalciferol, tetrahydrocannabinol, humulene, myrcene, Litsea cubeba oil, limonene, cranberry seed oil, raspberry seed oil, black cumin seed oil, retinyl palmitate, tocopheryl acetate, tocopherol, and combinations thereof.
4. The lipid nanoparticle dispersion of claim 1 having a latent lamellar structure.
5. The lipid nanoparticle dispersion of claim 1 that is a non-equilibrium lipid nanoparticle dispersion.
6. The lipid nanoparticle dispersion of claim 1 , wherein a concentration of water immiscible non-amphipathic compounds is greater than about 20%.
7. The lipid nanoparticle dispersion of claim 1 , further comprising a non-ethoxylated high hydrophile-lipophile-balance (HLB) surfactant selected from the group consisting of sodium lauryl sulfate, sodium stearoyl lactylate, and combinations thereof.
8. The lipid nanoparticle dispersion of claim 1 having a turbidity less than 250 nephelometric turbidity units (NTU) at room temperature.
9. The lipid nanoparticle dispersion of claim 1 having a turbidity less than 125 nephelometric turbidity units (NTU) at room temperature.
10. The lipid nanoparticle dispersion of claim 1 that does not include one or more co-solvents.
11. The lipid nanoparticle dispersion of claim 1 that does not include ibuprofen.
12. The lipid nanoparticle dispersion of claim 1 that does not include lidocaine.
13. The lipid nanoparticle dispersion of claim 1 wherein the lipid nanoparticles are not vesicular nanoparticles.
14. The lipid nanoparticle dispersion of claim 1 having a latent lamellar structure wherein the lipid nanoparticles are not vesicular nanoparticles.
15. The lipid nanoparticle dispersion of claim 1 having a volume average particle diameter in a range from 23.6 nm to 58 nm.
16. The lipid nanoparticle dispersion of claim 1 , further comprising a cryoprotectant.
17. The lipid nanoparticle dispersion of claim 16 , wherein the cryoprotectant comprises 6 to 9.8 wt. % glycerin.
18. The lipid nanoparticle dispersion of claim 1 , comprising more than one high HLB surfactant.
19. The lipid nanoparticle dispersion of claim 1 , wherein the one or more water immiscible oils comprises cyclopentasiloxane.
20. The lipid nanoparticle dispersion of claim 1 , wherein the one or more water immiscible oils comprises mineral oil.
21. The lipid nanoparticle dispersion of claim 3 , wherein the one or more hydrophobic therapeutic agents comprises limonene.
22. The lipid nanoparticle dispersion of claim 1 having a turbidity in a range from 47 to 365 nephelometric turbidity units (NTU) at room temperature.
23. A lipid nanoparticle dispersion comprising:
from about 10 to about 15 wt. % of one or more polyethoxylated high hydrophile-lipophile-balance (HLB) surfactants selected from the group consisting of laureth-23, ceteareth-20, ceteareth-30, steareth-40, PEG32 stearate, PEG75 stearate, PEG100 stearate, PEG15 hydroxy stearate, PEG40 hydrogenated castor oil, polysorbate 20, polysorbate 80, and combinations thereof;
from about 8 to about 12 wt. % of one or more low hydrophile-lipophile-balance (HLB) surfactants selected from the group consisting of cetyl alcohol, glyceryl oleate, glyceryl stearate, glyceryl monolinoleate, oleic acid, stearic acid, sorbitan oleate, soy lecithin, and combinations thereof;
from about 20 to about 40 wt. % of one or more water immiscible oils selected from the group consisting of medium chain triglyceride oil, coconut oil, isopropyl palmitate, isopropyl myristate, methyl decanoate, ethyl myristate, ethyl oleate, mineral oil, orange essential oil, cyclopentasiloxane, poly(dimethyl siloxane), hexadecane, propylene glycol dicaprylate, isododecane, isoeicosane, isohexadecane, soy biodiesel, jojoba oil, cocoyl caprylocaprate, C 10 -C 13 alkane, squalane, sunflower seed oil, and combinations thereof; and
from about 35 to about 60 wt. % water,
wherein the lipid nanoparticle dispersion has:
a lipophilic concentration greater than about 25%;
a volume average particle size less than about 100 nm; and
a turbidity less than 375 nephelometric turbidity units (NTU).