Electron microscope and image generation method
Provided is an electron microscope for generating a montage image by acquiring images of a plurality of regions in a montage image capturing region set on a specimen, and by connecting the acquired images. The electron microscope includes a specimen surface height calculating unit that calculates a distribution of specimen surface heights in the montage image capturing region by performing curved surface approximation based on the specimen surface heights determined by performing focus adjustment at a plurality of points set in a region including the montage image capturing region, and an image acquiring unit that acquires the images of the plurality of regions based on the calculated distribution of the specimen surface heights.
1. An electron microscope for generating a montage image by acquiring images of a plurality of regions in a montage image capturing region set on a specimen, and by connecting the acquired images, the electron microscope comprising:
a specimen surface height calculating unit that calculates a distribution of specimen surface heights in the montage image capturing region by performing curved surface approximation based on the specimen surface heights determined by performing focus adjustment at a plurality of points set in a region including the montage image capturing region; and
an image acquiring unit that acquires the images of the plurality of regions based on the calculated distribution of the specimen surface heights,
wherein the specimen surface heights are determined based on an excitation amount of an objective lens of the electron microscope or a height of a specimen stage of the electron microscope when each point of the plurality of points is in focus.
2. The electron microscope according to claim 1 , wherein
the specimen surface height calculating unit approximates the montage image capturing region to a rectangle, respectively sets four points among the plurality of points to positions near vertices of the rectangle, and respectively sets two points among the plurality of points to positions near mid-points of long sides of the rectangle.
3. The electron microscope according to claim 1 , wherein
in a case where a user has selected a first mode, the image acquiring unit receives an input from the user to specify a region at which auto focus adjustment is to be executed, from the plurality of regions, and executes the auto focus adjustment at the specified region when acquiring an image of the specified region, and
in a case where the user has selected a second mode, the image acquiring unit acquires the images of the plurality of regions based on the distribution of the specimen surface heights calculated by the specimen surface heights calculating unit.
4. An image generation method for generating a montage image by acquiring images of a plurality of regions in a montage image capturing region set on a specimen and by connecting the acquired images, the method comprising:
a specimen surface height calculating step of calculating a distribution of specimen surface heights in the montage image capturing region by performing curved surface approximation based on the specimen surface heights determined by performing focus adjustment at a plurality of points set in a region including the montage image capturing region; and
an image acquiring step of acquiring the images of the plurality of regions based on the calculated distribution of the specimen surface heights,
wherein the specimen surface heights are determined based on an excitation amount of an objective lens of an electron microscope or a height of a specimen stage of the electron microscope when each point of the plurality of points is in focus.