Optical measurement device and optical measurement system
There is provided an optical measurement device including a spectral optical system that disperses fluorescence emitted from a biological specimen, and an image sensor that receives the fluorescence dispersed by the spectral optical system and generates fluorescence information. The image sensor is divided into a plurality of regions that receive different wavelength components of the fluorescence and generate fluorescence information for each of the wavelength components.
1. An optical measurement device, comprising:
a spectral optical system configured to disperse fluorescence emitted from a biological specimen;
an image sensor configured to:
receive the fluorescence dispersed by the spectral optical system; and
generate fluorescence information, wherein
the image sensor is divided into a plurality of regions,
each region of the plurality of regions is configured to receive a different wavelength component of wavelength components of the fluorescence, wherein the generated fluorescence information comprises information corresponding to the received different wavelength component of the wavelength components, and
the fluorescence information is image data including a value obtained by addition of pixel values, for pixels of the image sensor, for each of the plurality of regions;
a signal acquisition unit configured to calculate, based on the addition of the pixel values for each of the plurality of regions, an evaluation value of the fluorescence information; and
an analysis unit configured to:
analyze the biological specimen based on the evaluation value of the fluorescence information by the signal acquisition unit; and
display the evaluation value.
2. The optical measurement device according to claim 1 , wherein the signal acquisition unit is further configured to:
divide the fluorescence information into a plurality of channel regions along a spectral direction of the fluorescence dispersed by the spectral optical system, wherein the plurality of channel regions corresponds to the plurality of regions.
3. The optical measurement device according to claim 1 , wherein the signal acquisition unit is further configured to
add pixel values of one or more filter regions in the fluorescence information.
4. The optical measurement device according to claim 1 , wherein
the spectral optical system includes a prism configured to disperse the fluorescence in a specific direction, and
a width of a first region of the plurality of regions in a direction corresponding to the specific direction is smaller than a width of a second region of the plurality of regions in the direction corresponding to the specific direction,
a first wavelength component of the fluorescence is incident on the first region, and
a second wavelength component of the fluorescence longer than the first wavelength component is incident on the second region.
5. The optical measurement device according to claim 1 , wherein
the image sensor includes:
a plurality of photoelectric conversion elements that are in a matrix, and
a detection circuit configured to generate the pixel values constituting the fluorescence information, based on charges generated in each of the plurality of photoelectric conversion elements.
6. The optical measurement device according to claim 1 , further comprising:
an excitation light source configured to irradiate the biological specimen with excitation light having a specific wavelength.
7. The optical measurement device according to claim 6 , further comprising:
a flow path through which the biological specimen flows, wherein
the excitation light source is further configured to irradiate a specific region in the flow path with the excitation light.
8. The optical measurement device according to claim 1 , further comprising:
an excitation light source configured to emit excitation light having a specific wavelength;
a stage on which the biological specimen is placed; and
a scanning mechanism configured to control a positional relation between the stage and the excitation light source such that the excitation light scans the biological specimen on the stage.
9. The optical measurement device according to claim 1 , wherein the analysis unit is further configured to acquire at least one of a type, a size, or a structure of the biological specimen based on the evaluation value.
10. An optical measurement system, comprising:
a spectral optical system configured to disperse fluorescence emitted from a biological specimen;
an image sensor configured to:
receive the fluorescence dispersed by the spectral optical system; and
generate fluorescence information, wherein
the image sensor includes a plurality of regions,
each region of the plurality of regions is configured to receive a different wavelength component of wavelength components of the fluorescence, wherein the generated fluorescence information comprises information corresponding to the received different wavelength component of the wavelength components, and
the fluorescence information is image data including a value obtained by addition of pixel values, for pixels of the image sensor, for each of the plurality of regions;
a signal acquisition unit configured to calculate, based on the addition of the pixel values for each of the plurality of regions, an evaluation value of the fluorescence information; and
an analysis unit configured to:
analyze the biological specimen based on the evaluation value of the fluorescence information by the signal acquisition unit; and
display the evaluation value.