IP Library › Granted Patent US 12,284,977
Granted Patent B2
US 12,284,977 · App. 18/163,499 · Granted Apr 29, 2025

Methods and compositions related to improved nitrogen utilization efficiency in tobacco

Inventors: Jesse Frederick (Richmond, VA); Chengalrayan Kudithipudi (Midlothian, VA); Dongmei Xu (Glen Allen, VA)
Assignee: Altria Client Services LLC
A01H6/823A01H5/10A01H5/12C07K14/415C12N9/1085C12N9/88C12N9/90C12N15/8227C12N15/8237C12N15/8261C12Y205/01054C12Y402/01033C12Y501/03015
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Quick Facts
Patent No.
US 12,284,977
App. No.
18/163,499
Granted
Apr 29, 2025
Kind
B2
Abstract

The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.

Claims (17)

1. A method of producing a Nicotiana tabacum plant comprising an enhanced nitrogen utilization efficiency (NUE) trait, said method comprising:

a) selecting a Nicotiana tabacum plant comprising at least one favorable NUE allele comprising a T nucleotide at a position corresponding to position 36 of SEQ ID NO: 61 or a T nucleotide at a position corresponding to position 36 of SEQ ID NO: 64;

b) crossing said Nicotiana tabacum plant selected in step a) with a second Nicotiana tabacum plant; and

c) obtaining progeny seed from a cross of step b) wherein a plant grown from said progeny seed comprises said enhanced NUE trait and said at least one favorable NUE allele.

2. The method of claim 1 , wherein said enhanced NUE trait is selected from the group consisting of an increased partial factor productivity (PFP), an increased agronomic efficiency (AE), an increased recovery efficiency (RE), an increased physiological efficiency (PE), and an increased internal efficiency (IE), when compared to a Nicotiana tabacum plant lacking said enhanced NUE trait when grown in the same conditions.

3. The method of claim 1 , wherein said Nicotiana tabacum plant is of a variety selected from the group consisting of MD609, MD601, Banket A1, K326, K346, K358, K394, K399, K730, NC196, NC37NF, NC471, NC55, NC92, NC2326, NC95, and NC925.

4. The method of claim 1 , wherein said second Nicotiana tabacum plant is a Burley tobacco variety.

5. The method of claim 4 , wherein said Burley tobacco variety is selected from the group consisting of TN86, TN86LC, TN90, TN90LC, TN97, and TN97LC.

6. A method of introgressing an enhanced nitrogen utilization efficiency (NUE) trait into a Nicotiana tabacum variety, said method comprising the steps of:

a) crossing a tobacco plant from a first Nicotiana tabacum variety comprising an enhanced NUE trait with a tobacco plant from a second Nicotiana tabacum variety lacking said enhanced NUE trait;

b) obtaining progeny seed from the cross of step a);

and

c) selecting at least one progeny seed comprising at least one favorable NUE allele comprising a T nucleotide at a position corresponding to position 36 of SEQ ID NO: 61 or a T nucleotide at a position corresponding to position 36 of SEQ ID NO: 64 and wherein a plant grown from said at least one progeny seed further comprises said enhanced NUE trait.

7. The method of claim 6 , wherein said second Nicotiana tabacum variety is a Burley tobacco variety.

8. The method of claim 7 , wherein said Burley tobacco variety is selected from the group consisting of TN86, TN86LC, TN90, TN90LC, TN97, and TN97LC.

9. The method of claim 6 , wherein said enhanced NUE trait is selected from the group consisting of an increased partial factor productivity (PFP), an increased agronomic efficiency (AE), an increased recovery efficiency (RE), an increased physiological efficiency (PE), and an increased internal efficiency (IE), when compared to a Nicotiana tabacum plant lacking said enhanced NUE trait when grown in the same conditions.

10. The method of claim 6 , wherein said first Nicotiana tabacum variety is selected from the group consisting of MD609, MD601, Banket A1, K326, K346, K358, K394, K399, K730, NC196, NC37NF, NC471, NC55, NC92, NC2326, NC95, and NC925.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 8, 2023
From: FREDERICK, JESSE; KUDITHIPUDI, CHENGALRAYAN; XU, DONGMEI
To: ALTRIA CLIENT SERVICES LLC
Reel/Frame 062628/0749 →
Continuity (4)
Continuation 17116816 · Dec 9, 2020
Continuation 16119366 · Aug 31, 2018
Provisional Application 62553501 · Sep 1, 2017
Related Publication 20240099257A1 · Mar 28, 2024
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