Fluorescence histo-tomography (FHT) systems and methods
A fluorescence histo-tomography (FHT) method may include capturing, using an imaging device positioned with respect to a chamber of a slicing apparatus, images of a block face of a specimen retained within the chamber. The images may be captured in the visible and infrared or near-infrared spectrum. The method may include co-registering the images to form a combined image, and providing the combined image to an electronic display. In some implementations, the method may include positioning the imaging device away from the chamber, operating the slicing apparatus to expose a second block face of the specimen, and repositioning the imaging device with respect to the chamber.
1. A method of performing fluorescence histo-tomography (FHT) comprising:
capturing, by an imaging device mounted with respect to a chamber of a slicing apparatus, a first image of a first block face of a specimen retained within the chamber, the first image captured in a visible spectrum;
capturing, by the imaging device, a second image of the first block face under white light and fluorescence excitation illumination;
positioning the imaging device away from the chamber;
operating the slicing apparatus to expose a second block face of the specimen;
repositioning the imaging device with respect to the chamber; and
capturing a third image of the second block face.
2. The method as in claim 1 , further comprising:
co-registering the first image and the second image to form a first combined image; and
providing the first combined image to an electronic display.
3. The method as in claim 1 , wherein the first image is captured under ambient light.
4. The method as in claim 1 , wherein the imaging device includes a handle to allow a user to removably position the imaging device with respect to the chamber.
5. The method as in claim 1 , further comprising:
prior to capturing the third image, directing a flow of pressurized gas towards the specimen to remove a slice of the specimen from a field of view of the imaging device.
6. The method as in claim 1 , wherein the imaging device is mounted within the chamber using a support mount in contact with a floor of the chamber.
7. The method as in claim 1 , wherein the first image and the second image are acquired simultaneously by the imaging device.
8. The method as in claim 1 , wherein the first image is capturing via a white light camera of the imaging device, and the second image is capturing via a fluorescence camera of the imaging device.
9. The method as in claim 1 , wherein the second image is captured in an infrared or near-infrared spectrum.
10. The method as in claim 1 , further comprising:
illuminating the first block face with light in the visible spectrum while capturing the first image.